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- catalog contributor b11460248.
- catalog created "c1999.".
- catalog date "2000".
- catalog date "c1999.".
- catalog dateCopyrighted "c1999.".
- catalog description "Includes bibliographical references and index.".
- catalog description "Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.".
- catalog extent "x, 306 p. :".
- catalog hasFormat "Transcription factor protocols.".
- catalog identifier "0896035735 (alk. paper)".
- catalog isFormatOf "Transcription factor protocols.".
- catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 130.".
- catalog isPartOf "Methods in molecular biology ; v. 130".
- catalog issued "2000".
- catalog issued "c1999.".
- catalog language "eng".
- catalog publisher "Totowa, N.J. : Humana Press,".
- catalog relation "Transcription factor protocols.".
- catalog subject "572.8/845 21".
- catalog subject "QH 450.2 T77155 2000".
- catalog subject "QP552.T68 T727 1999".
- catalog subject "Sequence Analysis, DNA methods.".
- catalog subject "Transcription Factors analysis.".
- catalog subject "Transcription factors Laboratory manuals.".
- catalog subject "Transfection methods.".
- catalog subject "W1 ME9616J v.130 2000".
- catalog tableOfContents "Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis.".
- catalog title "Transcription factor protocols / edited by Martin J. Tymms.".
- catalog type "Handbooks, manuals, etc. fast".
- catalog type "text".