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- catalog abstract ""The Fourth Edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting." "Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference."--Jacket.".
- catalog contributor b11492922.
- catalog created "c2000.".
- catalog date "2000".
- catalog date "c2000.".
- catalog dateCopyrighted "c2000.".
- catalog description ""The Fourth Edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting." "Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference."--Jacket.".
- catalog description "1. Introduction -- 2. Biology of Cultured Cells -- 3. Design and Layout -- 4. Equipment -- 5. Aseptic Technique -- 6. Safety -- 7. Culture Vessels -- 8. Media -- 9. Serum-Free Media -- 10. Preparation and Sterilization -- 11. Primary Culture -- 12. Cell Lines -- 13. Cloning and Selection -- 14. Cell Separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized Cells -- 23. Tumor Cells -- 24. Organotypic Culture -- 25. Scale-Up -- 26. Specialized Techniques -- 27. Molecular Techniques -- 28. Problem Solving.".
- catalog description "Includes bibliographical references (p. 523-561) and index.".
- catalog extent "xxvi, 577 p., [12] p. of plates :".
- catalog identifier "0471348899 (alk. paper)".
- catalog issued "2000".
- catalog issued "c2000.".
- catalog language "eng".
- catalog publisher "New York : Wiley-Liss,".
- catalog subject "571.6/38 21".
- catalog subject "Cell Culture Laboratory Manuals.".
- catalog subject "Cell Culture Techniques Laboratory Manuals.".
- catalog subject "Cell culture Laboratory manuals.".
- catalog subject "Cells, Cultured.".
- catalog subject "Culture Techniques methods.".
- catalog subject "QH 585.2 F885 2000".
- catalog subject "QH585.2 .F74 2000".
- catalog subject "Tissue Culture Laboratory Manuals.".
- catalog subject "Tissue culture Laboratory manuals.".
- catalog subject "Tissue culture.".
- catalog tableOfContents "1. Introduction -- 2. Biology of Cultured Cells -- 3. Design and Layout -- 4. Equipment -- 5. Aseptic Technique -- 6. Safety -- 7. Culture Vessels -- 8. Media -- 9. Serum-Free Media -- 10. Preparation and Sterilization -- 11. Primary Culture -- 12. Cell Lines -- 13. Cloning and Selection -- 14. Cell Separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized Cells -- 23. Tumor Cells -- 24. Organotypic Culture -- 25. Scale-Up -- 26. Specialized Techniques -- 27. Molecular Techniques -- 28. Problem Solving.".
- catalog title "Culture of animal cells : a manual of basic technique / R. Ian Freshney.".
- catalog type "Handbooks, manuals, etc. fast".
- catalog type "text".