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- catalog abstract ""An ever-increasing number of genes that are being sequenced have no known function. Determining the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable 'tricks of the trade' along the way. Protein Localization by Fluorescence Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding for the novice whilst also extending the range of the experienced user."--Jacket.".
- catalog contributor b11494448.
- catalog created "2000.".
- catalog date "2000".
- catalog date "2000.".
- catalog dateCopyrighted "2000.".
- catalog description ""An ever-increasing number of genes that are being sequenced have no known function. Determining the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable 'tricks of the trade' along the way. Protein Localization by Fluorescence Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding for the novice whilst also extending the range of the experienced user."--Jacket.".
- catalog description "1. Basic immunofluorescence / Victoria J. Allan -- 2. Immunofluorescent labelling of sections / Alison J. North and J. Victor Small -- 3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld and Elisa M. Stone -- 4. Instruments for fluorescence imaging / W.B. Amos -- 5. Fluorescence microscopy of living vertebrate cells / Rainer Pepperkok and David Shima -- 6. Visualizing fluorescence in Drosophila -- optimal detection in thick specimens / Ilan Davis -- 7. Green fluorescent protein in plants / Chris Hawes, Petra Boevink and Ian Moore -- 8. Fluorescence microscopy in yeast / Iain M. Hagan and Kathryn R. Ayscough -- App. A1. Excitation and emission maxima of commonly used fluorophores -- App. A2. Commercial sources of antibodies -- App. A3. Optical units -- App. A4. List of Suppliers.".
- catalog description "Includes bibliographical references and index.".
- catalog extent "xxiv, 231 p. :".
- catalog identifier "0199637407".
- catalog identifier "0199637415".
- catalog isPartOf "Practical approach series ; 218".
- catalog issued "2000".
- catalog issued "2000.".
- catalog language "eng".
- catalog publisher "Oxford ; New York : Oxford University Press,".
- catalog subject "572/.636 21".
- catalog subject "Fluorescence microscopy.".
- catalog subject "Green fluorescent protein.".
- catalog subject "Immunofluorescence.".
- catalog subject "Luminescent Proteins.".
- catalog subject "Microscopy, Fluorescence.".
- catalog subject "Proteins Analysis.".
- catalog subject "QP551 .P6965 2000".
- catalog subject "QU 55 P967 2000".
- catalog subject "Sequence Analysis, Protein.".
- catalog tableOfContents "1. Basic immunofluorescence / Victoria J. Allan -- 2. Immunofluorescent labelling of sections / Alison J. North and J. Victor Small -- 3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld and Elisa M. Stone -- 4. Instruments for fluorescence imaging / W.B. Amos -- 5. Fluorescence microscopy of living vertebrate cells / Rainer Pepperkok and David Shima -- 6. Visualizing fluorescence in Drosophila -- optimal detection in thick specimens / Ilan Davis -- 7. Green fluorescent protein in plants / Chris Hawes, Petra Boevink and Ian Moore -- 8. Fluorescence microscopy in yeast / Iain M. Hagan and Kathryn R. Ayscough -- App. A1. Excitation and emission maxima of commonly used fluorophores -- App. A2. Commercial sources of antibodies -- App. A3. Optical units -- App. A4. List of Suppliers.".
- catalog title "Protein localization by fluorescent microscopy : a practical approach / edited by Victoria J. Allan.".
- catalog type "text".