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- catalog contributor b12031753.
- catalog contributor b12031754.
- catalog created "c2001.".
- catalog date "2001".
- catalog date "c2001.".
- catalog dateCopyrighted "c2001.".
- catalog description "Includes bibliographical references and index.".
- catalog description "Overview: gene knockouts -- Isolation and maintenance of primate embryonic stem cells -- Gene targeting in ES cells -- Manipulating mouse embryonic stem cells -- Gene targeting in a centralized facility -- The loxP/CRE system and genome modification -- Creation and use of a Cre recombinase transgenic database -- Choice of mouse strains for gene targeting -- Isolation, microinjection, and transfer of mouse blastocysts -- Aggregatin chimeras: combining ES cells, diploid and tetraploid embryos -- How to study pathologic phenotypes of knockout mice -- Analysis of hematopoietic phenotypes in knockout mouse models -- Isolation of embryonic fibroblasts and their use in the in vitro characterization of gene function -- Influence of genetic background on knockout mouse phenotypes -- Lineage allocation during early embryogenesis: mapping of the neural primordia and application to the analysis of mouse mutants -- Generation of double-knockout, embryonic stem cells -- In vitro differentiation of embryonic stem cells and analysis of cellular phenotypes -- Embryonic stem cells in the study of hematopoiesis -- Interferon-inducible ES cell expression systems --Transgenic studies in the mouse: improving the technology towards a conditional temporal and spatial approach -- In vivo libraries of large insert transgenic mice for genetic mapping -- Epigenetic effects on transgene expression -- Positional-candidate cloning of genes from mouse mutants -- Genetically engineered mice: husbandry and resources -- Embryo cryopreservation for transgenic mouse lines.".
- catalog extent "xi, 431 p. :".
- catalog hasFormat "Gene knockout protocols.".
- catalog identifier "0896035727 (alk. paper)".
- catalog isFormatOf "Gene knockout protocols.".
- catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 158.".
- catalog isPartOf "Methods in molecular biology ; v. 158".
- catalog issued "2001".
- catalog issued "c2001.".
- catalog language "eng".
- catalog publisher "Totowa, N.J. : Humana Press,".
- catalog relation "Gene knockout protocols.".
- catalog subject "572.8/77 21".
- catalog subject "Gene Targeting methods Laboratory manuals.".
- catalog subject "Gene targeting Laboratory manuals.".
- catalog subject "Mice, Knockout Laboratory Manuals.".
- catalog subject "Mice, Knockout".
- catalog subject "Mice, Transgenic Laboratory Manuals.".
- catalog subject "QH 442.3 G325 2001".
- catalog subject "QH442.3 .G453 2000".
- catalog subject "Transgenic mice Laboratory manuals.".
- catalog subject "W1 ME9616J v.158 2001".
- catalog tableOfContents "Overview: gene knockouts -- Isolation and maintenance of primate embryonic stem cells -- Gene targeting in ES cells -- Manipulating mouse embryonic stem cells -- Gene targeting in a centralized facility -- The loxP/CRE system and genome modification -- Creation and use of a Cre recombinase transgenic database -- Choice of mouse strains for gene targeting -- Isolation, microinjection, and transfer of mouse blastocysts -- Aggregatin chimeras: combining ES cells, diploid and tetraploid embryos -- How to study pathologic phenotypes of knockout mice -- Analysis of hematopoietic phenotypes in knockout mouse models -- Isolation of embryonic fibroblasts and their use in the in vitro characterization of gene function -- Influence of genetic background on knockout mouse phenotypes -- Lineage allocation during early embryogenesis: mapping of the neural primordia and application to the analysis of mouse mutants -- Generation of double-knockout, embryonic stem cells -- In vitro differentiation of embryonic stem cells and analysis of cellular phenotypes -- Embryonic stem cells in the study of hematopoiesis -- Interferon-inducible ES cell expression systems --Transgenic studies in the mouse: improving the technology towards a conditional temporal and spatial approach -- In vivo libraries of large insert transgenic mice for genetic mapping -- Epigenetic effects on transgene expression -- Positional-candidate cloning of genes from mouse mutants -- Genetically engineered mice: husbandry and resources -- Embryo cryopreservation for transgenic mouse lines.".
- catalog title "Gene knockout protocols / edited by Martin J. Tymms and Ismail Kola.".
- catalog type "Handbooks, manuals, etc. fast".
- catalog type "Laboratory Manuals".
- catalog type "text".