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- catalog contributor b12317628.
- catalog created "c2002.".
- catalog date "2002".
- catalog date "c2002.".
- catalog dateCopyrighted "c2002.".
- catalog description "Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro.".
- catalog description "Includes bibliographical references and index.".
- catalog description "methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants.".
- catalog extent "xii, 377 p. :".
- catalog identifier "089603741X (alk. paper)".
- catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 181.".
- catalog isPartOf "Methods in molecular biology ; v. 181".
- catalog issued "2002".
- catalog issued "c2002.".
- catalog language "eng".
- catalog publisher "Totowa, N.J. : Humana Press,".
- catalog subject "572.8/6/028 21".
- catalog subject "Genomic Imprinting Laboratory Manuals.".
- catalog subject "Genomic Imprinting".
- catalog subject "Genomic imprinting Laboratory manuals.".
- catalog subject "QH 450 G335 2002".
- catalog subject "QH450 .G468 2001".
- catalog tableOfContents "Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro.".
- catalog tableOfContents "methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants.".
- catalog title "Genomic imprinting : methods and protocols / edited by Andrew Ward.".
- catalog type "Handbooks, manuals, etc. fast".
- catalog type "Laboratory Manuals".
- catalog type "text".