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• catalog contributor b12540328.
• catalog contributor b12540329.
• catalog created "c2002.".
• catalog date "2002".
• catalog date "c2002.".
• catalog dateCopyrighted "c2002.".
• catalog description "Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance pcr and magnetic beads / X. Huang, Z. Yuan and X. Cao -- Cloning pcr products. An overview / B. Guo and Y. Bi -- Using t4 DNA polymerase to generate clonable pcr products / K. Wang -- Enzyme-free cloning of pcr products and fusion protein expression / B.A. Neilan and D. Tillett -- Directional restriction site-free insertion of pcr products into vectors / G.J. Chen -- Autosticky pcr. Directional cloning of pcr products with performed 5' overhangs / J. Gal and M. Kalman -- A rapid and simple procedure for direct cloning of pcr products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould -- Pcr approaches to DNA mutagenesis and recombination. An overview / B. Shen -- In-frame cloning of synthetic genes using pcr inserts / J.C. Pierce -- Megaprimer pcr / S. Barik -- ".
• catalog description "Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong -- The isolation of DNA sequences flanking tn5 transposon insertions by inverse pcr / V.J. Martin and W.W. Mohn -- Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun -- Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored pcr / Z.N. Yang and T.E. Mirkov -- A "step down" pcr-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr -- Use of pcr in library screening. An overview / J. Zhu -- Cloning of homologous genes by gene-capture pcr / R. Mastrangeli and S. Donini -- Rapid and nonradioactive screening of recombinant libraries by pcr / M.W. King -- Rapid cdna cloning by pcr screening (rc-pcr) / T. Takumi -- ".
• catalog description "Generation and pcr screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli -- Pcr-based screening for bacterial artificial chromosome libraries / Y. Yasukochi -- A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang -- A microtiter-plate-based high throughput pcr product purification method / R. Smith and K. Wang.".
• catalog description "Includes bibliographical references and index.".
• catalog description "Pcr method for generating multiple mutations at adjacent sites / J. Adamec -- A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone -- Pcr screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque -- Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold -- Random mutagenesis by whole-plasmid pcr amplification / D. Kim and F.P. Guengerich -- Pcr-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo -- Long distance vectorette pcr (ldv pcr) / J.A. Fenton, G. Pratt and G.J. Morgan -- Nonspecific, nested suppression pcr method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli -- Inverse pcr. Cdna cloning / S.H. Huang -- Inverse pcr. Genomic DNA cloning / A.Y. Jong [and others] -- ".
• catalog description "Performing and optimizing PCR -- Mutagenesis and recombination -- Cloning unknown neighboring DNA -- Library construction and screening.".
• catalog description "Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch -- Computer programs for pcr primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen -- Single-step pcr optimization using touchdown and stepdown pcr programming / K.H. Roux -- Xl pcr amplification of long targets from genomic DNA / L.A. Kolmodin -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cdna fragments / J.T. Aatsinki -- Long distance reverse-transcription pcr / V. Thiel, J. Herold and S.G. Siddell -- Increasing pcr sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt -- Gc-rich template amplification by inverse pcr. DNA polymerase and solvent effects / A. Moreau [and others] -- Pcr procedure for the isolation of trinucleotide repeats / T. Tozaki -- Methylation-specific pcr / H. Ohashi -- ".
• catalog extent "xiv, 439 p. :".
• catalog hasFormat "PCR cloning protocols.".
• catalog identifier "0896039730 (comb. : alk. paper)".
• catalog isFormatOf "PCR cloning protocols.".
• catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 192.".
• catalog isPartOf "Methods in molecular biology ; v. 192".
• catalog issued "2002".
• catalog issued "c2002.".
• catalog language "eng".
• catalog publisher "Totowa, N.J. : Humana Press,".
• catalog relation "PCR cloning protocols.".
• catalog subject "572.8/6 21".
• catalog subject "Cloning, Molecular methods Laboratory Manuals.".
• catalog subject "Cloning, Molecular".
• catalog subject "Molecular cloning Laboratory manuals.".
• catalog subject "Mutagenesis genetics".
• catalog subject "Polymerase Chain Reaction methods Laboratory Manuals.".
• catalog subject "Polymerase Chain Reaction".
• catalog subject "Polymerase chain reaction Laboratory manuals.".
• catalog subject "QH 450.3 P3473 2002".
• catalog subject "QH442.2 .P37 2002".
• catalog subject "Recombination, Genetic".
• catalog subject "W1 ME9616J v.192 2002".
• catalog tableOfContents "Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance pcr and magnetic beads / X. Huang, Z. Yuan and X. Cao -- Cloning pcr products. An overview / B. Guo and Y. Bi -- Using t4 DNA polymerase to generate clonable pcr products / K. Wang -- Enzyme-free cloning of pcr products and fusion protein expression / B.A. Neilan and D. Tillett -- Directional restriction site-free insertion of pcr products into vectors / G.J. Chen -- Autosticky pcr. Directional cloning of pcr products with performed 5' overhangs / J. Gal and M. Kalman -- A rapid and simple procedure for direct cloning of pcr products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould -- Pcr approaches to DNA mutagenesis and recombination. An overview / B. Shen -- In-frame cloning of synthetic genes using pcr inserts / J.C. Pierce -- Megaprimer pcr / S. Barik -- ".
• catalog tableOfContents "Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong -- The isolation of DNA sequences flanking tn5 transposon insertions by inverse pcr / V.J. Martin and W.W. Mohn -- Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun -- Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored pcr / Z.N. Yang and T.E. Mirkov -- A "step down" pcr-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr -- Use of pcr in library screening. An overview / J. Zhu -- Cloning of homologous genes by gene-capture pcr / R. Mastrangeli and S. Donini -- Rapid and nonradioactive screening of recombinant libraries by pcr / M.W. King -- Rapid cdna cloning by pcr screening (rc-pcr) / T. Takumi -- ".
• catalog tableOfContents "Generation and pcr screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli -- Pcr-based screening for bacterial artificial chromosome libraries / Y. Yasukochi -- A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang -- A microtiter-plate-based high throughput pcr product purification method / R. Smith and K. Wang.".
• catalog tableOfContents "Pcr method for generating multiple mutations at adjacent sites / J. Adamec -- A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone -- Pcr screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque -- Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold -- Random mutagenesis by whole-plasmid pcr amplification / D. Kim and F.P. Guengerich -- Pcr-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo -- Long distance vectorette pcr (ldv pcr) / J.A. Fenton, G. Pratt and G.J. Morgan -- Nonspecific, nested suppression pcr method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli -- Inverse pcr. Cdna cloning / S.H. Huang -- Inverse pcr. Genomic DNA cloning / A.Y. Jong [and others] -- ".
• catalog tableOfContents "Performing and optimizing PCR -- Mutagenesis and recombination -- Cloning unknown neighboring DNA -- Library construction and screening.".
• catalog tableOfContents "Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch -- Computer programs for pcr primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen -- Single-step pcr optimization using touchdown and stepdown pcr programming / K.H. Roux -- Xl pcr amplification of long targets from genomic DNA / L.A. Kolmodin -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cdna fragments / J.T. Aatsinki -- Long distance reverse-transcription pcr / V. Thiel, J. Herold and S.G. Siddell -- Increasing pcr sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt -- Gc-rich template amplification by inverse pcr. DNA polymerase and solvent effects / A. Moreau [and others] -- Pcr procedure for the isolation of trinucleotide repeats / T. Tozaki -- Methylation-specific pcr / H. Ohashi -- ".
• catalog title "PCR cloning protocols / edited by Bing-Yuan Chen and Harry W. Janes.".
• catalog type "Handbooks, manuals, etc. fast".
• catalog type "Laboratory Manuals".
• catalog type "text".