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- catalog contributor b12736165.
- catalog created "c2003.".
- catalog date "2003".
- catalog date "c2003.".
- catalog dateCopyrighted "c2003.".
- catalog description "Complementary DNA libraries: an overview / Shao-Yao Ying -- Rapid amplification of cDNA ends / Yue Zhang -- cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones -- Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen -- Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz [and others] -- Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong -- Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara -- Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano -- cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji -- Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz -- ".
- catalog description "Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva [and others] -- Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying -- Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin -- Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura -- Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge -- Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin -- Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge -- Future perspectives / Shao-Yao Ying.".
- catalog description "Includes bibliographical references and index.".
- catalog description "Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin -- mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying -- Quality assessment of cDNA libraries / Hans-Jürgen Fülle -- Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying -- PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz -- Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin -- Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han -- Screening poly (dA/dT( -- )) cDNA for gene identification / San Ming Wang [and others] -- Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen [and others] -- ".
- catalog extent "xiii, 335 p. :".
- catalog identifier "1588290662 (alk. paper)".
- catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 221.".
- catalog isPartOf "Methods in molecular biology ; v. 221".
- catalog issued "2003".
- catalog issued "c2003.".
- catalog language "eng".
- catalog publisher "Totowa, N.J. : Humana Press,".
- catalog subject "611/.01816 21".
- catalog subject "Antisense DNA Laboratory manuals.".
- catalog subject "Combinatorial Chemistry Techniques methods Laboratory Manuals.".
- catalog subject "DNA, Antisense Laboratory Manuals.".
- catalog subject "DNA, Complementary".
- catalog subject "Gene Library Laboratory Manuals.".
- catalog subject "Gene Library".
- catalog subject "Genetic Techniques".
- catalog subject "Molecular Biology methods".
- catalog subject "QP624.5.A57 G46 2003".
- catalog subject "QU 25 G326 2003".
- catalog subject "W1 ME9616J v.221 2003".
- catalog tableOfContents "Complementary DNA libraries: an overview / Shao-Yao Ying -- Rapid amplification of cDNA ends / Yue Zhang -- cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones -- Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen -- Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz [and others] -- Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong -- Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara -- Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano -- cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji -- Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz -- ".
- catalog tableOfContents "Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva [and others] -- Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying -- Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin -- Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura -- Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge -- Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin -- Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge -- Future perspectives / Shao-Yao Ying.".
- catalog tableOfContents "Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin -- mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying -- Quality assessment of cDNA libraries / Hans-Jürgen Fülle -- Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying -- PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz -- Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin -- Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han -- Screening poly (dA/dT( -- )) cDNA for gene identification / San Ming Wang [and others] -- Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen [and others] -- ".
- catalog title "Generation of cDNA libraries : methods and protocols / edited by Shao-Yao Ying.".
- catalog type "Handbooks, manuals, etc. fast".
- catalog type "Laboratory Manuals".
- catalog type "text".