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catalog abstract "Provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications.".
catalog contributor b12928914.
catalog contributor b12928915.
catalog created "c2003.".
catalog date "2003".
catalog date "c2003.".
catalog dateCopyrighted "c2003.".
catalog description "A short history of the polymerase chain reaction / J.M. Bartlett and D. Stirling -- Pcr patent issues / P. Carroll and D. Casimir -- Equipping and establishing a pcr laboratory / S. McDonagh -- Quality control in pcr / D. Stirling -- Extraction of nucleic acid templates / J.M. Bartlett -- Extraction of DNA from whole blood / J.M. Bartlett and A. White -- DNA extraction from tissue / H. Pearson and D. Stirling -- Extraction of DNA from microdissected archival tissues / J.J. Going -- Rna extraction from blood / H. Pearson -- Rna extraction from frozen tissue / J.M. Bartlett -- Rna extraction from tissue sections / H. Pearson -- Dual DNA/rna extraction / D. Stirling and J.M. Bartlett -- DNA extraction from fungi, yeast, and bacteria / D. Stirling -- Isolation of rna viruses from biological materials / S. McDonagh -- Extraction of ancient DNA / W.M. Schmerer -- DNA extraction from plasma and serum / D. Stirling -- Technical notes for the detection of nucleic acids / J.M. Bartlett -- ".
catalog description "Cloning gene family members using pcr with degenerate oligonucleotide primers / G.M. Preston -- Cdna libraries from a low amount of cells / P. Ravassard ... [et al.] -- Creation of chimeric junctions, deletions, and insertions by pcr / G. Pont-Kingdon -- Recombination and site-directed mutagenesis using recombination pcr / D.H. Jones and S.C. Winistorfer -- Megaprimer pcr: Application in mutagenesis and gene fusion / E. Burke and S. Barik.".
catalog description "Includes bibliographical references and index.".
catalog description "Long pcr methodology / R. Tellier ... [et al.] -- Qualitative and quantitative pcr: A technical overview / D. Stirling -- Ultrasensitive pcr detection of tumor cells in myeloma / F.W. Cremer and M. Moos -- Ultrasensitive quantitative pcr to detect rna viruses / S. McDonagh -- Quantitative pcr for camp ri alpha mrna: Use of site-directed mutation and pcr mimics / J.M. Bartlett -- Quantitation of multiple rna species / R. Kerr -- Differential display: A technical overview / J.M. Bartlett -- Au-differential display, reproducibility of a differential mrna display targeted to au motifs / O. Dominguez ... [et al.] -- Pcr fluorescence differential display / K. Khalturin, S. Kuznetsov and T.C. Bosch -- Microarray analysis using rna arbitrarily primed pcr / S. Ringquist ... [et al.] -- Oligonucleotide arrays for genotyping: Enzymatic methods for typing single nucleotide polymorphisms and short tandem repeats / S. Case-Green, C. Pritchard and E. Southern -- ".
catalog description "Pcr-based detection of nucleic acids in chromosomes, cells, and tissues: Technical considerations on prins and in situ pcr and comparison with in situ hybridization / E.J. Speel, F.C. Ramaekers and A.H. Hopman -- Cycling primed in situ amplification / J.H. Bull and L. Paskins -- Direct and indirect in situ pcr / K.H. Wiedorn and T. Goldmann -- Reverse transcriptase in situ pcr: New methods in cellular interrogation / M. Gilchrist and A.D. Befus -- Primed in situ nucleic acid labeling combined with immunocytochemistry to simultaneously localize DNA and proteins in cells and chromosomes / E.J. Speel, F.C. Ramaekers and A.H. Hopman -- Cloning and mutagenesis: A technical overview / H. Pearson and D. Stirling -- Using t4 DNA polymerase to generate clonable pcr products / K. Wang -- A t-linker strategy for modification and directional cloning of pcr products / R.M. Horton, R. Raju and B.M. Conti-Fine -- ".
catalog description "Pcr-sscp analysis of polymorphism: A simple and sensitive method for detecting differences between short segments of DNA / M. Han and M.A. Robinson -- Sequencing: A technical overview / D. Stirling -- Preparation and direct automated cycle sequencing of pcr products / S.E. Daniels -- Nonradioactive pcr sequencing using digoxigenin / S. Kosel ... [et al.] -- Direct sequencing by thermal asymmetric pcr / G.R. Mazars and C. Theillet -- Analysis of nucleotide sequence variations by solid-phase minisequencing / A. Suomalainen and A.C. Syvanen -- Direct sequencing with highly degenerate and inosine-containing primers / Z. Shen ... [et al.] -- Determination of unknown genomic sequences without cloning / J.P. Quivy and P.B. Becker -- Cloning pcr products for sequencing in m13 vectors / D. Walsh -- DNA rescue by the vectorette method / M.A. McAleer, A.J. Coffey and I. Dunham -- Technical notes for sequencing difficult templates / D. Stirling -- ".
catalog description "Provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications.".
catalog description "Serial analysis of gene expression / K.A. Oien -- Mutation and polymorphism detection: A technical overview / J. Edwards and J.M. Bartlett -- Combining multiplex and touchdown pcr for microsatellite analysis / K. Rithidech and J.J. Dunn -- Detection of microsatellite instability and loss of heterozygosity using DNA extracted from formalin-fixed paraffin-embedded tumor material by fluorescence-based multiplex microsatellite pcr / J. Edwards and J.M. Bartlett -- Reduction of shadow band synthesis during pcr amplification of repetitive sequences from modern and ancient DNA / W.M. Schmerer -- Degenerate oligonucleotide-primed pcr / M. Aubele and J. Smida -- Mutation detection using rt-pcr-rflp / H. Nakashima, M. Akahoshi and Y. Tanaka -- Multiplex amplification refractory mutation system for the detection of prothrombotic polymorphisms / D. Stirling -- ".
catalog description "Technical notes for the recovery and purification of pcr products from acrylamide gels / D. Stirling -- Pcr primer design / D.L. Hyndman and M. Mitsuhashi -- Optimization of polymerase chain reactions / H. Grunenwald -- Subcycling pcr for long-distance amplifications of regions with high and low guanine-cystine content: Amplification of the intron 22 inversion of the fviii gene / D. Stirling -- Rapid amplification of cdna ends / X. Wang and W.S. Young, 3rd -- Randomly amplified polymorphic DNA fingerprinting: The basics / R.S. Dassanayake and L.P. Samaranayake -- Microsphere-based single nucleotide polymorphism genotyping / M.A. Iannone ... [et al.] -- Ligase chain reaction / W.H. Benjamin, Jr., K.R. Smith and K.B. Waites -- Nested rt-pcr in a single closed tube / A. Olmos ... [et al.] -- Direct pcr from serum: Application to viral genome detection / K. Abe -- Long pcr amplification of large fragments of viral genomes: A technical overview / R. Tellier ... [et al.] -- ".
catalog extent "xvii, 545 p. :".
catalog hasFormat "PCR protocols.".
catalog identifier "0896036421 (alk. paper)".
catalog isFormatOf "PCR protocols.".
catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 226.".
catalog isPartOf "Methods in molecular biology ; v. 226".
catalog issued "2003".
catalog issued "c2003.".
catalog language "eng".
catalog publisher "Totowa, N.J. : Humana Press,".
catalog relation "PCR protocols.".
catalog subject "572/.43 21".
catalog subject "Genetic Engineering methods".
catalog subject "Nucleic Acid Amplification Techniques".
catalog subject "Polymerase Chain Reaction methods.".
catalog subject "Polymerase Chain Reaction".
catalog subject "Polymerase chain reaction Laboratory manuals.".
catalog subject "QH 450.3 P3479 2003".
catalog subject "QP606.D46 P3595 2003".
catalog subject "W1 ME9616J v.226 2003".
catalog tableOfContents "A short history of the polymerase chain reaction / J.M. Bartlett and D. Stirling -- Pcr patent issues / P. Carroll and D. Casimir -- Equipping and establishing a pcr laboratory / S. McDonagh -- Quality control in pcr / D. Stirling -- Extraction of nucleic acid templates / J.M. Bartlett -- Extraction of DNA from whole blood / J.M. Bartlett and A. White -- DNA extraction from tissue / H. Pearson and D. Stirling -- Extraction of DNA from microdissected archival tissues / J.J. Going -- Rna extraction from blood / H. Pearson -- Rna extraction from frozen tissue / J.M. Bartlett -- Rna extraction from tissue sections / H. Pearson -- Dual DNA/rna extraction / D. Stirling and J.M. Bartlett -- DNA extraction from fungi, yeast, and bacteria / D. Stirling -- Isolation of rna viruses from biological materials / S. McDonagh -- Extraction of ancient DNA / W.M. Schmerer -- DNA extraction from plasma and serum / D. Stirling -- Technical notes for the detection of nucleic acids / J.M. Bartlett -- ".
catalog tableOfContents "Cloning gene family members using pcr with degenerate oligonucleotide primers / G.M. Preston -- Cdna libraries from a low amount of cells / P. Ravassard ... [et al.] -- Creation of chimeric junctions, deletions, and insertions by pcr / G. Pont-Kingdon -- Recombination and site-directed mutagenesis using recombination pcr / D.H. Jones and S.C. Winistorfer -- Megaprimer pcr: Application in mutagenesis and gene fusion / E. Burke and S. Barik.".
catalog tableOfContents "Long pcr methodology / R. Tellier ... [et al.] -- Qualitative and quantitative pcr: A technical overview / D. Stirling -- Ultrasensitive pcr detection of tumor cells in myeloma / F.W. Cremer and M. Moos -- Ultrasensitive quantitative pcr to detect rna viruses / S. McDonagh -- Quantitative pcr for camp ri alpha mrna: Use of site-directed mutation and pcr mimics / J.M. Bartlett -- Quantitation of multiple rna species / R. Kerr -- Differential display: A technical overview / J.M. Bartlett -- Au-differential display, reproducibility of a differential mrna display targeted to au motifs / O. Dominguez ... [et al.] -- Pcr fluorescence differential display / K. Khalturin, S. Kuznetsov and T.C. Bosch -- Microarray analysis using rna arbitrarily primed pcr / S. Ringquist ... [et al.] -- Oligonucleotide arrays for genotyping: Enzymatic methods for typing single nucleotide polymorphisms and short tandem repeats / S. Case-Green, C. Pritchard and E. Southern -- ".
catalog tableOfContents "Pcr-based detection of nucleic acids in chromosomes, cells, and tissues: Technical considerations on prins and in situ pcr and comparison with in situ hybridization / E.J. Speel, F.C. Ramaekers and A.H. Hopman -- Cycling primed in situ amplification / J.H. Bull and L. Paskins -- Direct and indirect in situ pcr / K.H. Wiedorn and T. Goldmann -- Reverse transcriptase in situ pcr: New methods in cellular interrogation / M. Gilchrist and A.D. Befus -- Primed in situ nucleic acid labeling combined with immunocytochemistry to simultaneously localize DNA and proteins in cells and chromosomes / E.J. Speel, F.C. Ramaekers and A.H. Hopman -- Cloning and mutagenesis: A technical overview / H. Pearson and D. Stirling -- Using t4 DNA polymerase to generate clonable pcr products / K. Wang -- A t-linker strategy for modification and directional cloning of pcr products / R.M. Horton, R. Raju and B.M. Conti-Fine -- ".
catalog tableOfContents "Pcr-sscp analysis of polymorphism: A simple and sensitive method for detecting differences between short segments of DNA / M. Han and M.A. Robinson -- Sequencing: A technical overview / D. Stirling -- Preparation and direct automated cycle sequencing of pcr products / S.E. Daniels -- Nonradioactive pcr sequencing using digoxigenin / S. Kosel ... [et al.] -- Direct sequencing by thermal asymmetric pcr / G.R. Mazars and C. Theillet -- Analysis of nucleotide sequence variations by solid-phase minisequencing / A. Suomalainen and A.C. Syvanen -- Direct sequencing with highly degenerate and inosine-containing primers / Z. Shen ... [et al.] -- Determination of unknown genomic sequences without cloning / J.P. Quivy and P.B. Becker -- Cloning pcr products for sequencing in m13 vectors / D. Walsh -- DNA rescue by the vectorette method / M.A. McAleer, A.J. Coffey and I. Dunham -- Technical notes for sequencing difficult templates / D. Stirling -- ".
catalog tableOfContents "Serial analysis of gene expression / K.A. Oien -- Mutation and polymorphism detection: A technical overview / J. Edwards and J.M. Bartlett -- Combining multiplex and touchdown pcr for microsatellite analysis / K. Rithidech and J.J. Dunn -- Detection of microsatellite instability and loss of heterozygosity using DNA extracted from formalin-fixed paraffin-embedded tumor material by fluorescence-based multiplex microsatellite pcr / J. Edwards and J.M. Bartlett -- Reduction of shadow band synthesis during pcr amplification of repetitive sequences from modern and ancient DNA / W.M. Schmerer -- Degenerate oligonucleotide-primed pcr / M. Aubele and J. Smida -- Mutation detection using rt-pcr-rflp / H. Nakashima, M. Akahoshi and Y. Tanaka -- Multiplex amplification refractory mutation system for the detection of prothrombotic polymorphisms / D. Stirling -- ".
catalog tableOfContents "Technical notes for the recovery and purification of pcr products from acrylamide gels / D. Stirling -- Pcr primer design / D.L. Hyndman and M. Mitsuhashi -- Optimization of polymerase chain reactions / H. Grunenwald -- Subcycling pcr for long-distance amplifications of regions with high and low guanine-cystine content: Amplification of the intron 22 inversion of the fviii gene / D. Stirling -- Rapid amplification of cdna ends / X. Wang and W.S. Young, 3rd -- Randomly amplified polymorphic DNA fingerprinting: The basics / R.S. Dassanayake and L.P. Samaranayake -- Microsphere-based single nucleotide polymorphism genotyping / M.A. Iannone ... [et al.] -- Ligase chain reaction / W.H. Benjamin, Jr., K.R. Smith and K.B. Waites -- Nested rt-pcr in a single closed tube / A. Olmos ... [et al.] -- Direct pcr from serum: Application to viral genome detection / K. Abe -- Long pcr amplification of large fragments of viral genomes: A technical overview / R. Tellier ... [et al.] -- ".
catalog title "PCR protocols / edited by John M.S. Bartlett and David Stirling.".
catalog type "Handbooks, manuals, etc. fast".
catalog type "Laboratory Manuals".
catalog type "text".