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• catalog abstract "Presents state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium. This book offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.".
• catalog contributor b12928933.
• catalog created "c2003.".
• catalog date "2003".
• catalog date "c2003.".
• catalog dateCopyrighted "c2003.".
• catalog description "Immunogold detection of microvascular proteins in the compromised blood-brain barrier / E.M. Cornford, S. Hyman and M.E. Cornford -- Measurement of blood-brain and blood-tumor barrier permeabilities with [14C]-labeled tracers / K. Asotra, N. Ningaraj and K.L. Black -- A review of blood-brain barrier transport techniques / Q.R. Smith -- In situ brain perfusion technique / Q.R. Smith and D.D. Allen -- Brain efflux index method. Characterization of efflux transport across the blood-brain barrier / H. Kusuhara, T. Terasaki and Y. Sugiyama -- Transport studies using membrane vesicles / D.R. Peterson and R.A. Hawkins -- Drug transport studies using quantitative microdialysis / H. Dai and W.F. Elmquist -- Single-pass dual-label indicator method. Blood-to-brain transport of glucose and short-chain monocarboxylic acids / M.A. Puchowicz, K. Xu and J.C. LaManna -- Protein transport in cerebral endothelium. In vitro transcytosis of transferrin / L. Fenart and R. Cecchelli -- ".
• catalog description "Includes bibliographical references and index.".
• catalog description "Morphology and molecular properties of cellular components of normal cerebral vessels / S. Nag -- Studies of cerebral vessels by transmission electron microscopy and morphometry / S. Nag -- Freeze-fracture studies of cerebral endothelial tight junctions / H. Wolburg, S. Liebner and A. Lippoldt -- Studies of cerebral endothelium by scanning and high-voltage electron microscopy / A.S. Lossinsky and R.R. Shivers -- Detection of endothelial/lymphocyte interaction in spinal cord microvasculature by intravital videomicroscopy / B. Engelhardt, P. Vajkoczy and M. Laschinger -- Pathophysiology of blood-brain barrier breakdown / S. Nag -- Pial vessel permeability to tracers using cranial windows / W.G. Mayhan -- Blood-brain barrier permeability using tracers and immunohistochemistry / S. Nag -- Ultracytochemical studies of the compromised blood-brain barrier / S. Nag -- ".
• catalog description "Phage display technology for identifying specific antigens on brain endothelial cells / J. Tanha, A. Muruganandam and D. Stanimirovic -- mRNA detection in cerebral vessels by nonradioactive in situ hybridization / D. Zagzag and W. Chan -- Proteomics of brain endothelium. Separation of proteins by two-dimensional gel electrophoresis and identification by mass spectrometry / R.F. Haseloff, E. Krause and I.E. Blasig -- Immunoblot detection of brain vascular proteins / C.T. Matson and L.R. Drewes -- Immunohistochemical detection of endothelial proteins / S. Nag -- Cerebral vascular function in genetically altered mice / F.M. Faraci -- Generation of transgenic mice by pronuclear injection / A. Damert and H. Kusserow -- Mouse genome modification / R. Rozmahel.".
• catalog description "Presents state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium. This book offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.".
• catalog description "Transport across the choroid plexuses in vivo and in vitro / N. Strazielle and J.E. Preston -- An overview of in vitro techniques for blood-brain barrier studies / A. Reichel, D.J. Begley and N.J. Abbott -- Isolation and characterization of human brain endothelial cells / K. Dorovini-Zis, R. Prameya and H. Huynh -- Human brain microvessel endothelial cell and leukocyte interactions / J. Shukaliak-Quandt, D. Wong and K. Dorovini-Zis -- Development and characterization of immortalized cerebral endothelial cell lines / P.O. Couraud ... [et al.] -- Isolation and characterization of retinal endothelial cells / D.A. Antonetti and E.B. Wolpert -- Isolation and characterization of cerebral microvascular pericytes / P. Dore-Duffy -- Molecular biology of the blood-brain barrier / W.M. Pardridge -- Blood-brain barrier genomics / R.J. Boado -- Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis / M. Marroni ... [et al.] -- ".
• catalog extent "xiii, 549 p. :".
• catalog identifier "1588290735 (alk. paper)".
• catalog isPartOf "Methods in molecular medicine ; 89".
• catalog issued "2003".
• catalog issued "c2003.".
• catalog language "eng".
• catalog publisher "Totowa, N.J. : Humana Press,".
• catalog subject "612.8/24 21".
• catalog subject "Blood-Brain Barrier physiology.".
• catalog subject "Blood-brain barrier Laboratory manuals.".
• catalog subject "Clinical Protocols.".
• catalog subject "Molecular Biology methods.".
• catalog subject "Molecular Diagnostic Techniques methods.".
• catalog subject "QP375.5 .B528 2003".
• catalog subject "W1 ME9616JM v.89 2003".
• catalog subject "WL 200 B6547 2003".
• catalog tableOfContents "Immunogold detection of microvascular proteins in the compromised blood-brain barrier / E.M. Cornford, S. Hyman and M.E. Cornford -- Measurement of blood-brain and blood-tumor barrier permeabilities with [14C]-labeled tracers / K. Asotra, N. Ningaraj and K.L. Black -- A review of blood-brain barrier transport techniques / Q.R. Smith -- In situ brain perfusion technique / Q.R. Smith and D.D. Allen -- Brain efflux index method. Characterization of efflux transport across the blood-brain barrier / H. Kusuhara, T. Terasaki and Y. Sugiyama -- Transport studies using membrane vesicles / D.R. Peterson and R.A. Hawkins -- Drug transport studies using quantitative microdialysis / H. Dai and W.F. Elmquist -- Single-pass dual-label indicator method. Blood-to-brain transport of glucose and short-chain monocarboxylic acids / M.A. Puchowicz, K. Xu and J.C. LaManna -- Protein transport in cerebral endothelium. In vitro transcytosis of transferrin / L. Fenart and R. Cecchelli -- ".
• catalog tableOfContents "Morphology and molecular properties of cellular components of normal cerebral vessels / S. Nag -- Studies of cerebral vessels by transmission electron microscopy and morphometry / S. Nag -- Freeze-fracture studies of cerebral endothelial tight junctions / H. Wolburg, S. Liebner and A. Lippoldt -- Studies of cerebral endothelium by scanning and high-voltage electron microscopy / A.S. Lossinsky and R.R. Shivers -- Detection of endothelial/lymphocyte interaction in spinal cord microvasculature by intravital videomicroscopy / B. Engelhardt, P. Vajkoczy and M. Laschinger -- Pathophysiology of blood-brain barrier breakdown / S. Nag -- Pial vessel permeability to tracers using cranial windows / W.G. Mayhan -- Blood-brain barrier permeability using tracers and immunohistochemistry / S. Nag -- Ultracytochemical studies of the compromised blood-brain barrier / S. Nag -- ".
• catalog tableOfContents "Phage display technology for identifying specific antigens on brain endothelial cells / J. Tanha, A. Muruganandam and D. Stanimirovic -- mRNA detection in cerebral vessels by nonradioactive in situ hybridization / D. Zagzag and W. Chan -- Proteomics of brain endothelium. Separation of proteins by two-dimensional gel electrophoresis and identification by mass spectrometry / R.F. Haseloff, E. Krause and I.E. Blasig -- Immunoblot detection of brain vascular proteins / C.T. Matson and L.R. Drewes -- Immunohistochemical detection of endothelial proteins / S. Nag -- Cerebral vascular function in genetically altered mice / F.M. Faraci -- Generation of transgenic mice by pronuclear injection / A. Damert and H. Kusserow -- Mouse genome modification / R. Rozmahel.".
• catalog tableOfContents "Transport across the choroid plexuses in vivo and in vitro / N. Strazielle and J.E. Preston -- An overview of in vitro techniques for blood-brain barrier studies / A. Reichel, D.J. Begley and N.J. Abbott -- Isolation and characterization of human brain endothelial cells / K. Dorovini-Zis, R. Prameya and H. Huynh -- Human brain microvessel endothelial cell and leukocyte interactions / J. Shukaliak-Quandt, D. Wong and K. Dorovini-Zis -- Development and characterization of immortalized cerebral endothelial cell lines / P.O. Couraud ... [et al.] -- Isolation and characterization of retinal endothelial cells / D.A. Antonetti and E.B. Wolpert -- Isolation and characterization of cerebral microvascular pericytes / P. Dore-Duffy -- Molecular biology of the blood-brain barrier / W.M. Pardridge -- Blood-brain barrier genomics / R.J. Boado -- Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis / M. Marroni ... [et al.] -- ".
• catalog title "The blood-brain barrier : biology and research protocols / edited by Sukriti Nag.".
• catalog type "Handbooks, manuals, etc. fast".
• catalog type "text".