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• catalog abstract "Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination.".
• catalog contributor b13012594.
• catalog created "c2004.".
• catalog date "2004".
• catalog date "c2004.".
• catalog dateCopyrighted "c2004.".
• catalog description "Basic principles of cancer cell culture / S.P. Langdon -- Essential techniques of cancer cell culture / K.G. MacLeod and S.P. Langdon -- Characterization and authentication of cancer cell lines: an overview / S.P. Langdon -- Authentication of cancer cell lines by DNA fingerprinting / W.G. Dirks and H.G. Drexler -- Cytogenetic characterization of tumor cell lines / R.A. MacLeod and H.G. Drexler -- Isolation and culture of colon cancer cell lines / J.G. Park, J.L. Ku and S.Y. Park -- Isolation and culture of melanoma cell lines / A.I. Riker -- Isolation and culture of human brain tumor cells / G.G. Gomez and C.A. Kruse -- Isolation and culture of renal cancer cell lines / J.G. Park, J.L. Ku and S.Y. Park -- Isolation and culture of prostate cancer cell lines / N.M. Navone, M. Olive and P. Troncoso -- Isolation and culture of ovarian cancer cell lines / S.P. Langdon -- Isolation and culture of leukemia cell lines / H.G. Drexler -- ".
• catalog description "Cell sensitivity assays: clonogenic assay / J.A. Plumb -- Cell sensitivity assays: the MTT assay / J.A. Plumb -- PARP cleavage as a means of assessing apoptosis / P. Mullen -- Detection of apoptosis by the TUNEL assay / J. Lawry -- Apoptosis measurement by annexin v staining / E. Miller -- Cell adhesion assays / W.N. Scott -- Radial monolayer cell migration assay / M.E. Berens and C. Beaudry -- Invasion and motility assays / W.N. Scott -- Identification of senescence in cancer cells / L.J. Jardine -- Angiogenesis assays / W.N. Scott -- Flow cytometric DNA analysis of human cancer cell lines / P. Mullen -- DNA-mediated gene transfer / A.J. O'Donnell -- Development of drug-resistant models / H.M. Coley -- Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene / N.J. Maitland ... [et al.] -- The use of Matrigel to facilitate the establishment of human cancer cell lines as xenografts / P. Mullen -- ".
• catalog description "Coculture of prostate cancer cells / S.H. Lang and N.J. Maitland -- Coculture of ovarian cells using porous tissue culture inserts / K.E. Snow and S.P. Langdon -- Cell culture contamination: an overview / S.P. Langdon -- Detecting Mycoplasma contamination in cell cultures by polymerase chain reaction / C.C. Uphoff and H.G. Drexler -- Elimination of Mycoplasma from infected cell lines using antibiotics / C.C. Uphoff and H.G. Drexler.".
• catalog description "Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination.".
• catalog description "Includes bibliographical references and index.".
• catalog extent "xii, 360 p. :".
• catalog identifier "1588290794 (alk. paper)".
• catalog isPartOf "Methods in molecular medicine ; 88".
• catalog issued "2004".
• catalog issued "c2004.".
• catalog language "eng".
• catalog publisher "Totowa, N.J. : Humana Press,".
• catalog subject "616.99/407 21".
• catalog subject "Cancer cells Laboratory manuals.".
• catalog subject "Clinical Laboratory Techniques Laboratory Manuals.".
• catalog subject "Clinical Laboratory Techniques".
• catalog subject "QZ 25 C215 2003".
• catalog subject "RC267 .C336 2003".
• catalog subject "Tumor Cells, Cultured Laboratory Manuals.".
• catalog subject "Tumor Cells, Cultured".
• catalog subject "W1 ME9616JM v.88 2003".
• catalog tableOfContents "Basic principles of cancer cell culture / S.P. Langdon -- Essential techniques of cancer cell culture / K.G. MacLeod and S.P. Langdon -- Characterization and authentication of cancer cell lines: an overview / S.P. Langdon -- Authentication of cancer cell lines by DNA fingerprinting / W.G. Dirks and H.G. Drexler -- Cytogenetic characterization of tumor cell lines / R.A. MacLeod and H.G. Drexler -- Isolation and culture of colon cancer cell lines / J.G. Park, J.L. Ku and S.Y. Park -- Isolation and culture of melanoma cell lines / A.I. Riker -- Isolation and culture of human brain tumor cells / G.G. Gomez and C.A. Kruse -- Isolation and culture of renal cancer cell lines / J.G. Park, J.L. Ku and S.Y. Park -- Isolation and culture of prostate cancer cell lines / N.M. Navone, M. Olive and P. Troncoso -- Isolation and culture of ovarian cancer cell lines / S.P. Langdon -- Isolation and culture of leukemia cell lines / H.G. Drexler -- ".
• catalog tableOfContents "Cell sensitivity assays: clonogenic assay / J.A. Plumb -- Cell sensitivity assays: the MTT assay / J.A. Plumb -- PARP cleavage as a means of assessing apoptosis / P. Mullen -- Detection of apoptosis by the TUNEL assay / J. Lawry -- Apoptosis measurement by annexin v staining / E. Miller -- Cell adhesion assays / W.N. Scott -- Radial monolayer cell migration assay / M.E. Berens and C. Beaudry -- Invasion and motility assays / W.N. Scott -- Identification of senescence in cancer cells / L.J. Jardine -- Angiogenesis assays / W.N. Scott -- Flow cytometric DNA analysis of human cancer cell lines / P. Mullen -- DNA-mediated gene transfer / A.J. O'Donnell -- Development of drug-resistant models / H.M. Coley -- Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene / N.J. Maitland ... [et al.] -- The use of Matrigel to facilitate the establishment of human cancer cell lines as xenografts / P. Mullen -- ".
• catalog tableOfContents "Coculture of prostate cancer cells / S.H. Lang and N.J. Maitland -- Coculture of ovarian cells using porous tissue culture inserts / K.E. Snow and S.P. Langdon -- Cell culture contamination: an overview / S.P. Langdon -- Detecting Mycoplasma contamination in cell cultures by polymerase chain reaction / C.C. Uphoff and H.G. Drexler -- Elimination of Mycoplasma from infected cell lines using antibiotics / C.C. Uphoff and H.G. Drexler.".
• catalog title "Cancer cell culture : methods and protocols / edited by Simon P. Langdon.".
• catalog type "Handbooks, manuals, etc. fast".
• catalog type "Laboratory Manuals".
• catalog type "text".