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catalog abstract "A collection of state-of-the-art methods for interrogating ligand-receptor and receptor-effector interactions. Methods emphasize the regulation of G protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G proteins, and approaches to studying how receptor expression and function is regulated. The easily reproducible protocols include chimeric receptor construction, antibody generation, transient and stable transfection methods, hybridization, as well as immunocytochemistry, functional assays of G protein function, and antisense methods for defining receptor-effector coupling pathways.".
catalog contributor b13147232.
catalog contributor b13147233.
catalog created "c2004.".
catalog date "2004".
catalog date "c2004.".
catalog dateCopyrighted "c2004.".
catalog description "A collection of state-of-the-art methods for interrogating ligand-receptor and receptor-effector interactions. Methods emphasize the regulation of G protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G proteins, and approaches to studying how receptor expression and function is regulated. The easily reproducible protocols include chimeric receptor construction, antibody generation, transient and stable transfection methods, hybridization, as well as immunocytochemistry, functional assays of G protein function, and antisense methods for defining receptor-effector coupling pathways.".
catalog description "Covalent modification of g-proteins by affinity labeling / M. Hohenegger, M. Freissmuth and C. Nanoff -- Measurement of agonist-stimulated [35s]gtpgammas binding to assess total g-protein and galpha-subtype-specific activation by g-protein-coupled receptors / M.R. Dowling, S.R. Nahorski and R.A. Challiss -- Identification and quantitation of g-protein alpha-subunits / I. Mullaney and G. Milligan -- Analysis of function of receptor-g-protein and receptor-rgs fusion proteins / R.J. Ward and G. Milligan -- Assessment of receptor internalization and recycling / J.A. Koenig -- G-protein-coupled receptor phosphorylation and palmitoylation / A.B. Tobin and M. Wheatley -- Identification of g-protein-coupled receptor phosphorylation sites by 2d phosphopeptide mapping / A. Blaukat -- Ubiquitination of g-protein-coupled receptors / A. Marchese and J.L. Benovic -- Receptor mutagenesis strategies for examination of structure-function relationships / M. Blomenrohr, H.F. Vischer and J. Bogerd -- ".
catalog description "Includes bibliographical references and index.".
catalog description "Radioligand-binding methods for membrane preparations and intact cells / D.B. Bylund, J.D. Deupree and M.L. Toews -- Determination of allosteric interactions using radioligand-binding techniques / S. Lazareno -- Generation, use, and validation of receptor-selective antibodies / J.J. Mackrill -- Immunocytochemical identification of g-protein-coupled receptor expression and localization / L.C. Mongan and B.D. Grubb -- Generation and use of epitope-tagged receptors / R.A. McIlhinney -- Identification of g-protein-coupled receptor mrna expression by northern blotting and in situ hybridization / L.F. Donaldson -- Electron microscopic studies of receptor localization / R. Lujan -- Generation of model cell lines expressing recombinant g-protein-coupled receptors / E. Hermans -- Viral infection protocols / A. Porcellini and A. De Blasi -- Expression of g-protein signaling components in adult mammalian neurons by microinjection / S.R. Ikeda -- ".
catalog description "Study of g-protein-coupled receptor-protein interactions by bioluminescence resonance energy transfer / K.M. Kroeger and K.A. Eidne -- Fluorescence resonance energy transfer to study receptor dimerization in living cells / J.E. Bader and A.G. Beck-Sickinger -- Identification of protein interactions by yeast two-hybrid screening and coimmunoprecipitation / M. Tanowitz and M. von Zastrow -- Study of g-protein-coupled receptor-protein interactions using gel overlay assays and glutathione-s-transferase-fusion protein pull-downs / A.E. Brady, Q. Wang and L.E. Limbird -- Receptor knock-out and knock-in strategies / I. Matsuda and A. Aiba -- Statistical methods in g-protein-coupled receptor research / P. Freeman and D. Spina.".
catalog extent "xiii, 418 p. :".
catalog identifier "1588293297 (alk. paper)".
catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 259.".
catalog isPartOf "Methods in molecular biology ; v. 259".
catalog issued "2004".
catalog issued "c2004.".
catalog language "eng".
catalog publisher "Totowa, N.J. : Humana Press,".
catalog subject "572/.69 22".
catalog subject "Cell receptors Research Methodology.".
catalog subject "Cellular signal transduction Research Methodology.".
catalog subject "QH 601 R2954 2004".
catalog subject "QH603.C43 R42 2004".
catalog subject "Signal Transduction physiology Laboratory Manuals.".
catalog subject "Signal Transduction physiology".
catalog subject "W1 ME9616J v.259 2004".
catalog tableOfContents "Covalent modification of g-proteins by affinity labeling / M. Hohenegger, M. Freissmuth and C. Nanoff -- Measurement of agonist-stimulated [35s]gtpgammas binding to assess total g-protein and galpha-subtype-specific activation by g-protein-coupled receptors / M.R. Dowling, S.R. Nahorski and R.A. Challiss -- Identification and quantitation of g-protein alpha-subunits / I. Mullaney and G. Milligan -- Analysis of function of receptor-g-protein and receptor-rgs fusion proteins / R.J. Ward and G. Milligan -- Assessment of receptor internalization and recycling / J.A. Koenig -- G-protein-coupled receptor phosphorylation and palmitoylation / A.B. Tobin and M. Wheatley -- Identification of g-protein-coupled receptor phosphorylation sites by 2d phosphopeptide mapping / A. Blaukat -- Ubiquitination of g-protein-coupled receptors / A. Marchese and J.L. Benovic -- Receptor mutagenesis strategies for examination of structure-function relationships / M. Blomenrohr, H.F. Vischer and J. Bogerd -- ".
catalog tableOfContents "Radioligand-binding methods for membrane preparations and intact cells / D.B. Bylund, J.D. Deupree and M.L. Toews -- Determination of allosteric interactions using radioligand-binding techniques / S. Lazareno -- Generation, use, and validation of receptor-selective antibodies / J.J. Mackrill -- Immunocytochemical identification of g-protein-coupled receptor expression and localization / L.C. Mongan and B.D. Grubb -- Generation and use of epitope-tagged receptors / R.A. McIlhinney -- Identification of g-protein-coupled receptor mrna expression by northern blotting and in situ hybridization / L.F. Donaldson -- Electron microscopic studies of receptor localization / R. Lujan -- Generation of model cell lines expressing recombinant g-protein-coupled receptors / E. Hermans -- Viral infection protocols / A. Porcellini and A. De Blasi -- Expression of g-protein signaling components in adult mammalian neurons by microinjection / S.R. Ikeda -- ".
catalog tableOfContents "Study of g-protein-coupled receptor-protein interactions by bioluminescence resonance energy transfer / K.M. Kroeger and K.A. Eidne -- Fluorescence resonance energy transfer to study receptor dimerization in living cells / J.E. Bader and A.G. Beck-Sickinger -- Identification of protein interactions by yeast two-hybrid screening and coimmunoprecipitation / M. Tanowitz and M. von Zastrow -- Study of g-protein-coupled receptor-protein interactions using gel overlay assays and glutathione-s-transferase-fusion protein pull-downs / A.E. Brady, Q. Wang and L.E. Limbird -- Receptor knock-out and knock-in strategies / I. Matsuda and A. Aiba -- Statistical methods in g-protein-coupled receptor research / P. Freeman and D. Spina.".
catalog title "Receptor signal transduction protocols / edited by Gary B. Willars, R. A. John Challiss.".
catalog type "Aufsatzsammlung. swd".
catalog type "Laboratory Manuals".
catalog type "text".