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• catalog abstract "Updated with many of the new approaches that have been transforming the field, including readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. The book offers step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necesary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.".
• catalog contributor b13290220.
• catalog contributor b13290221.
• catalog created "c2004.".
• catalog date "2004".
• catalog date "c2004.".
• catalog dateCopyrighted "c2004.".
• catalog description "Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES-MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- Measurement of protein/DNA interactions in vivo by chromatin immunoprecipitation -- Hogune Im ... [et al.] -- Characterization of protein/DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- Nonradioactive methods for detecting activation of ras-related small G proteins / Douglas A. Andres.".
• catalog description "Includes bibliographical references and index.".
• catalog description "Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- Signal transduction inhibitors in cellular function / Maofu Fu ... [et al.] -- Two-dimensional gel electrophoresis for the identification of signaling targets -- Yukihito Kabuyama ... [et al.] -- A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski -- Determining protein half-lives / Pengbo Zhou -- Assaying protein kinase activity / Jan Brbek and Steven K. Hanks.".
• catalog description "Measuring dynamic changes in camp using fluorescence resonance energy transfer / Sandrine Evellin ... [et al.] -- In vivo detection of protein/protein interaction in plant cells using BRET / Chitra Subramanian ... [et al.] -- Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- Assaying cytochrome C translocation during apoptosis / Nigel J. Waterhouse ... [et al.].".
• catalog description "Nucleocytoplasmic glycosylation, o-glcnac: identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart -- Techniques in protein methylation / Jaeho Lee, Donghang Cheng, and Mark T. Bedford -- Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- Assaying phospholipase A2 activity / Christina C. Leslie and Michael H. Gelb -- Measurement and immunofluorescence of cellular phosphinositides / Hiroko Hama ... [et al.].".
• catalog description "Updated with many of the new approaches that have been transforming the field, including readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. The book offers step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necesary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.".
• catalog extent "xii, 327 p. :".
• catalog identifier "1588292452 (alk. paper)".
• catalog isPartOf "Methods in molecular biology (Clifton, N.J.) ; 284.".
• catalog isPartOf "Methods in molecular biology ; v. 284".
• catalog issued "2004".
• catalog issued "c2004.".
• catalog language "eng".
• catalog publisher "Totowa, N.J. : Humana Press,".
• catalog subject "Cell receptors Laboratory manuals.".
• catalog subject "Cellular signal transduction Laboratory manuals.".
• catalog subject "G proteins Laboratory manuals.".
• catalog subject "GTP-Binding Proteins analysis Laboratory Manuals.".
• catalog subject "GTP-Binding Proteins analysis".
• catalog subject "QP517.C45 S555 2004".
• catalog subject "QU 25 S578 2004".
• catalog subject "Signal Transduction physiology Laboratory Manuals.".
• catalog subject "Signal Transduction physiology".
• catalog subject "W1 ME9616J v.284 2004".
• catalog tableOfContents "Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES-MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- Measurement of protein/DNA interactions in vivo by chromatin immunoprecipitation -- Hogune Im ... [et al.] -- Characterization of protein/DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- Nonradioactive methods for detecting activation of ras-related small G proteins / Douglas A. Andres.".
• catalog tableOfContents "Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- Signal transduction inhibitors in cellular function / Maofu Fu ... [et al.] -- Two-dimensional gel electrophoresis for the identification of signaling targets -- Yukihito Kabuyama ... [et al.] -- A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski -- Determining protein half-lives / Pengbo Zhou -- Assaying protein kinase activity / Jan Brbek and Steven K. Hanks.".
• catalog tableOfContents "Measuring dynamic changes in camp using fluorescence resonance energy transfer / Sandrine Evellin ... [et al.] -- In vivo detection of protein/protein interaction in plant cells using BRET / Chitra Subramanian ... [et al.] -- Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- Assaying cytochrome C translocation during apoptosis / Nigel J. Waterhouse ... [et al.].".
• catalog tableOfContents "Nucleocytoplasmic glycosylation, o-glcnac: identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart -- Techniques in protein methylation / Jaeho Lee, Donghang Cheng, and Mark T. Bedford -- Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- Assaying phospholipase A2 activity / Christina C. Leslie and Michael H. Gelb -- Measurement and immunofluorescence of cellular phosphinositides / Hiroko Hama ... [et al.].".
• catalog title "Signal transduction protocols / edited by Robert C. Dickson and Michael D. Mendenhall.".
• catalog type "Aufsatzsammlung. swd".
• catalog type "Handbooks, manuals, etc. fast".
• catalog type "Laboratory Manuals".
• catalog type "text".