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2001029437 contributor B8931734.
2001029437 created "c2001.".
2001029437 date "2001".
2001029437 date "c2001.".
2001029437 dateCopyrighted "c2001.".
2001029437 description "Includes bibliographical references (p. [69]-76) and index.".
2001029437 description "Machine generated contents note: LIST OF FIGURESXI -- LIST OF TABLESXIII -- PREFACE XV -- ACKNOWLEDGEMENTS XIX -- GENERAL INTRODUCTION 1 -- 1. FEMALE MEIOTIC CELL CYCLE 1 -- 2. GENESIS AND MEIOSIS IN DROSOPHILA FEMALES 4 -- 3. CELL CYCLE REGULATION OF DROSOPHILA FEMALE MEIOSIS5 -- 4. MUTATIONS IN GRAUZONE (GRAU) AND CORTEX(CORT) BLOCK PROGRESSION OF DROSOPHILA FEMALE MEIOSIS AND CAUSE OTHER DEFECTS 6 -- 5. GOALS AND SIGNIFICANCE OF THIS DISSERTATION RESEARCH 7 -- MAPPING OF DROSOPHILA MUTATIONS USING SITE-SPECIFIC MALE RECOMBINATION 11 -- 1. INTRODUCTION 11 -- 2. MATERIALS AND METHODS13 -- 2.1 Drosophila stocks 13 -- 2.2 Complementation tests ofgrau mutations with chromosomal deficiencies and Pelement insertion lines15 -- 2.3 Generating recombinants by P-element-induced male recombination 15 -- 2.4 Fertility tests of Pelement induced male recombinants16 -- 3. RESULTS 17 -- 3.1 Scheme for mapping Drosophila mutations using Pelement induced male recombination 17 -- 3.2 Mapping the grau gene 19 -- 3.3 Mapping the cort gene.22 -- 4. DISCUSSIO N 27 -- COMPLETION OF MEIOSIS IN DROSOPHILA OOCYTES REQUIRES TRANSCRIPTIONAL CONTROL BY GRAUZONE, A NEW ZINC FINGER PROTEIN 31 -- 1. INTRODUCTION 31 -- 2. EXPERIMENTAL PROCEDURES 33 -- 2.1 Fly stocks and mapping33 -- 2.2 Genetic test between grau and cort 34 -- 2.3 P-element-mediated transformation and rescue35 -- 2.4 Molecular techniques 35 -- 2.5 Grau-GFP construct35 -- 2.6 Microscopy 36 -- 2.7 Construction and purification of GST- Grau fusion protein37 -- 2.8 Gel shift assay 37 -- 3. RESULTS38 -- 3.1 Isolation of the grau gene38 -- 3.2 The Grau protein 42 -- 3.3 Nature of the grau mutant alleles44 -- 3.4 Expression of the grau transcript during development46 -- 3.5 The Grau-GFP fusion protein is localized to the nucleus of both nurse cells andfollicle cells46 -- 3.6 Interaction between grau and cort 47 -- 3.7 Grau protein binds to the cort promoter51 -- 4. DISCUSSION 53 -- 4.1 Mutations in the grau alleles affect the coding region of the protein 53 -- 4.2 Regulation of Grau function during oogenesis and at later developmental stages54 -- 4.3 Control of meiotic divisions55 -- 4.4 Translational control, meiosis, and early development 57 -- 5. ACKNOWLEDGEMENTS 59 -- CONCLUSIONS AND CONSIDERATIONS 61 -- 1. DROSOPHILA AS A MODEL TO STUDY REGULATION OF FEMALE MEIOTIC CELLCYCLE 6 1 -- 2. GRAU AND CORT ARE TWO MEMBERS OF A NEW PATHWAY THAT REGULATES THE MEIOTIC CELL CYCLE MACHINERY IN DROSOPHILA -- FEMALES 62 -- 3. MALE RECOMBINATION MAPPING IS A GENERAL METHOD TO MAP DROSOPHILA AUTOSOMAL MUTATIONS 66 -- REFERENCES-- INDEX77.".
2001029437 extent "xx, 80 p. :".
2001029437 identifier "0792373634 (alk. paper)".
2001029437 identifier 2001029437-b.html.
2001029437 identifier 2001029437-d.html.
2001029437 identifier 2001029437.html.
2001029437 issued "2001".
2001029437 issued "c2001.".
2001029437 language "eng".
2001029437 publisher "Boston : Kluwer Academic Publishers,".
2001029437 subject "571.8/45 21".
2001029437 subject "Drosophila cytology.".
2001029437 subject "Drosophila.".
2001029437 subject "Meiosis genetics.".
2001029437 subject "Meiosis.".
2001029437 subject "Oogenesis physiology.".
2001029437 subject "Oogenesis.".
2001029437 subject "QH 605.3 C518g 2001".
2001029437 subject "QP552.Z55 C474 2001".
2001029437 subject "Zinc Fingers genetics.".
2001029437 subject "Zinc-finger proteins.".
2001029437 tableOfContents "Machine generated contents note: LIST OF FIGURESXI -- LIST OF TABLESXIII -- PREFACE XV -- ACKNOWLEDGEMENTS XIX -- GENERAL INTRODUCTION 1 -- 1. FEMALE MEIOTIC CELL CYCLE 1 -- 2. GENESIS AND MEIOSIS IN DROSOPHILA FEMALES 4 -- 3. CELL CYCLE REGULATION OF DROSOPHILA FEMALE MEIOSIS5 -- 4. MUTATIONS IN GRAUZONE (GRAU) AND CORTEX(CORT) BLOCK PROGRESSION OF DROSOPHILA FEMALE MEIOSIS AND CAUSE OTHER DEFECTS 6 -- 5. GOALS AND SIGNIFICANCE OF THIS DISSERTATION RESEARCH 7 -- MAPPING OF DROSOPHILA MUTATIONS USING SITE-SPECIFIC MALE RECOMBINATION 11 -- 1. INTRODUCTION 11 -- 2. MATERIALS AND METHODS13 -- 2.1 Drosophila stocks 13 -- 2.2 Complementation tests ofgrau mutations with chromosomal deficiencies and Pelement insertion lines15 -- 2.3 Generating recombinants by P-element-induced male recombination 15 -- 2.4 Fertility tests of Pelement induced male recombinants16 -- 3. RESULTS 17 -- 3.1 Scheme for mapping Drosophila mutations using Pelement induced male recombination 17 -- 3.2 Mapping the grau gene 19 -- 3.3 Mapping the cort gene.22 -- 4. DISCUSSIO N 27 -- COMPLETION OF MEIOSIS IN DROSOPHILA OOCYTES REQUIRES TRANSCRIPTIONAL CONTROL BY GRAUZONE, A NEW ZINC FINGER PROTEIN 31 -- 1. INTRODUCTION 31 -- 2. EXPERIMENTAL PROCEDURES 33 -- 2.1 Fly stocks and mapping33 -- 2.2 Genetic test between grau and cort 34 -- 2.3 P-element-mediated transformation and rescue35 -- 2.4 Molecular techniques 35 -- 2.5 Grau-GFP construct35 -- 2.6 Microscopy 36 -- 2.7 Construction and purification of GST- Grau fusion protein37 -- 2.8 Gel shift assay 37 -- 3. RESULTS38 -- 3.1 Isolation of the grau gene38 -- 3.2 The Grau protein 42 -- 3.3 Nature of the grau mutant alleles44 -- 3.4 Expression of the grau transcript during development46 -- 3.5 The Grau-GFP fusion protein is localized to the nucleus of both nurse cells andfollicle cells46 -- 3.6 Interaction between grau and cort 47 -- 3.7 Grau protein binds to the cort promoter51 -- 4. DISCUSSION 53 -- 4.1 Mutations in the grau alleles affect the coding region of the protein 53 -- 4.2 Regulation of Grau function during oogenesis and at later developmental stages54 -- 4.3 Control of meiotic divisions55 -- 4.4 Translational control, meiosis, and early development 57 -- 5. ACKNOWLEDGEMENTS 59 -- CONCLUSIONS AND CONSIDERATIONS 61 -- 1. DROSOPHILA AS A MODEL TO STUDY REGULATION OF FEMALE MEIOTIC CELLCYCLE 6 1 -- 2. GRAU AND CORT ARE TWO MEMBERS OF A NEW PATHWAY THAT REGULATES THE MEIOTIC CELL CYCLE MACHINERY IN DROSOPHILA -- FEMALES 62 -- 3. MALE RECOMBINATION MAPPING IS A GENERAL METHOD TO MAP DROSOPHILA AUTOSOMAL MUTATIONS 66 -- REFERENCES-- INDEX77.".
2001029437 title "Grauzone and completion of meiosis during drosophila oogenesis / by Bin Chen.".
2001029437 type "text".