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- 2013940920 contributor B12831446.
- 2013940920 contributor B12831447.
- 2013940920 date "2013".
- 2013940920 description "Includes bibliographical references and index.".
- 2013940920 description "Part I Overview of microglia biology -- Brief overview of multitalented microglia / Bertrand Joseph and José Luis Venero -- Part II Isolation and culture of microglia -- Cell culturing of human and murine microglia cell lines / Johanna Rodhe -- Microglia isolation from adult mouse brain / Jae-Kyung Lee and Malú G. Tansey -- Preparation of primary microglia cultures from postnatal mouse and rat brains / Tomas Deierborg -- Isolation of murine postnatal brain microglia for phenotypic characterization using magnetic cell separation technology / Ashley S. Harms and Malú G. Tansey -- Isolation and culture of adult human microglia within mixed glial cultures for functional experimentation and high-content analysis / Amy M. Smith ... [et al.] -- Part III Depletion and transduction of microglia -- Depletion of microglia from primary cellular cultures / Lorena Pont-Lezica, Sabrina Colasse, and Alain Bessis -- Lentiviral transduction of cultured microglia / Takahiro Masuda ... [et al.] -- Part IV Analysis of microglial cytokine production -- Microglial activation : measurement of cytokines by flow cytometry / Deepak Kumar Kaushik and Anirban Basu -- In situ hybridization of cytokine mRNA using alkaline phosphatase-labelled oligodeoxynucleotide probes / Bettina Clausen, Christina Fenger, and Bente Finsen -- Use of meso-scale discovery to examine cytokine content in microglia cell supernatant / Miguel A. Burguillos -- Part V Analysis of microglia activation -- Analysis of microglial production of reactive oxygen and nitrogen species / Urte Neniskyte and Guy C. Brown -- Quantification of active caspase-3 and active caspase-8 in microglia cells / Edel Kavanagh -- Quantification of microglial phagocytosis by a flow cytometer-based assay / Refik Pul, Kandiyil Prajeeth Chittappen, and Martin Stangel -- Quantification of microglial proliferation and apoptosis by flow cytometry / Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen -- Fluorescence imaging of intracellular Ca2+, Na+, and H+ in cultured microglia / Tom Schilling and Claudia Eder -- Patch clamp protocols to study ion channel activity in microglia / Tom Schilling and Claudia Eder -- Part VI Analysis of microglia polarization -- Studying M1 and M2 states in adult microglia / Sadanand M. Gaikwad and Michael T. Heneka -- Isolating, culturing, and polarizing primary human adult and fetal microglia / Bryce A. Durafourt ... [et al.] -- Part VII Co-culture systems to analysis microglia interactions with other cell types -- Understanding microglia-neuron cross talk : relevance of the microglia-neuron cocultures / Fernando G. Correa, Miriam Hernangómez, and Carmen Guaza -- Preparation of rodent primary cultures for neuron-glia, mixed glia, enriched microglia, and reconstituted cultures with microglia / Shih-Heng Chen, Esteban A. Oyarzabal, and Jau-Shyong Hong -- Part VIII Analysis of microglia functions in vivo -- Microglia detection by enzymatic histochemistry / Beatriz Almolda, Berta González, and Bernardo Castellano -- Tomato lectin histochemistry for microglial visualization / Nàdia Villacampa ... [et al.] -- Immunohistochemical detection of microglia / Rocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera -- Intrathecal Infusion of Microglia Cells / Takahiro Masuda ... [et al.] -- Intracranial injection of LPS in rat as animal model of neuroinflammation / Ana M. Espinosa-Oliva, Rocío M. de Pablos, and Antonio J. Herrera -- Analyses of microglia effector function using CX3CR1-GFP knock-in mice / Jenny A. Garcia, Sandra M. Cardona, and Astrid E. Cardona -- In vivo two-photon microscopy of microglia / Satoru Kondo and Shigeo Okabe -- Use of confocal microscopy in the study of microglia in a brain metastasis model / Manuel Sarmiento.".
- 2013940920 extent "xii, 350 pages :".
- 2013940920 identifier "1627035192 (alk. paper)".
- 2013940920 identifier "9781627035194 (alk. paper)".
- 2013940920 isPartOf "Methods in molceular biology, 1064-3745 ; 1041".
- 2013940920 isPartOf "Methods in molecular biology (Clifton, N.J.) ; v. 1041.".
- 2013940920 isPartOf "Springer protocols (Series)".
- 2013940920 isPartOf "Springer protocols".
- 2013940920 issued "2013".
- 2013940920 language "eng".
- 2013940920 subject "612.8/2 23".
- 2013940920 subject "Central Nervous System cytology Laboratory Manuals.".
- 2013940920 subject "Microglia Laboratory Manuals.".
- 2013940920 subject "Microglia.".
- 2013940920 subject "QP370 .M53 2013".
- 2013940920 tableOfContents "Part I Overview of microglia biology -- Brief overview of multitalented microglia / Bertrand Joseph and José Luis Venero -- Part II Isolation and culture of microglia -- Cell culturing of human and murine microglia cell lines / Johanna Rodhe -- Microglia isolation from adult mouse brain / Jae-Kyung Lee and Malú G. Tansey -- Preparation of primary microglia cultures from postnatal mouse and rat brains / Tomas Deierborg -- Isolation of murine postnatal brain microglia for phenotypic characterization using magnetic cell separation technology / Ashley S. Harms and Malú G. Tansey -- Isolation and culture of adult human microglia within mixed glial cultures for functional experimentation and high-content analysis / Amy M. Smith ... [et al.] -- Part III Depletion and transduction of microglia -- Depletion of microglia from primary cellular cultures / Lorena Pont-Lezica, Sabrina Colasse, and Alain Bessis -- Lentiviral transduction of cultured microglia / Takahiro Masuda ... [et al.] -- Part IV Analysis of microglial cytokine production -- Microglial activation : measurement of cytokines by flow cytometry / Deepak Kumar Kaushik and Anirban Basu -- In situ hybridization of cytokine mRNA using alkaline phosphatase-labelled oligodeoxynucleotide probes / Bettina Clausen, Christina Fenger, and Bente Finsen -- Use of meso-scale discovery to examine cytokine content in microglia cell supernatant / Miguel A. Burguillos -- Part V Analysis of microglia activation -- Analysis of microglial production of reactive oxygen and nitrogen species / Urte Neniskyte and Guy C. Brown -- Quantification of active caspase-3 and active caspase-8 in microglia cells / Edel Kavanagh -- Quantification of microglial phagocytosis by a flow cytometer-based assay / Refik Pul, Kandiyil Prajeeth Chittappen, and Martin Stangel -- Quantification of microglial proliferation and apoptosis by flow cytometry / Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen -- Fluorescence imaging of intracellular Ca2+, Na+, and H+ in cultured microglia / Tom Schilling and Claudia Eder -- Patch clamp protocols to study ion channel activity in microglia / Tom Schilling and Claudia Eder -- Part VI Analysis of microglia polarization -- Studying M1 and M2 states in adult microglia / Sadanand M. Gaikwad and Michael T. Heneka -- Isolating, culturing, and polarizing primary human adult and fetal microglia / Bryce A. Durafourt ... [et al.] -- Part VII Co-culture systems to analysis microglia interactions with other cell types -- Understanding microglia-neuron cross talk : relevance of the microglia-neuron cocultures / Fernando G. Correa, Miriam Hernangómez, and Carmen Guaza -- Preparation of rodent primary cultures for neuron-glia, mixed glia, enriched microglia, and reconstituted cultures with microglia / Shih-Heng Chen, Esteban A. Oyarzabal, and Jau-Shyong Hong -- Part VIII Analysis of microglia functions in vivo -- Microglia detection by enzymatic histochemistry / Beatriz Almolda, Berta González, and Bernardo Castellano -- Tomato lectin histochemistry for microglial visualization / Nàdia Villacampa ... [et al.] -- Immunohistochemical detection of microglia / Rocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera -- Intrathecal Infusion of Microglia Cells / Takahiro Masuda ... [et al.] -- Intracranial injection of LPS in rat as animal model of neuroinflammation / Ana M. Espinosa-Oliva, Rocío M. de Pablos, and Antonio J. Herrera -- Analyses of microglia effector function using CX3CR1-GFP knock-in mice / Jenny A. Garcia, Sandra M. Cardona, and Astrid E. Cardona -- In vivo two-photon microscopy of microglia / Satoru Kondo and Shigeo Okabe -- Use of confocal microscopy in the study of microglia in a brain metastasis model / Manuel Sarmiento.".
- 2013940920 title "Microglia : methods and protocols / edited by Bertrand Joseph, Department of Oncology-Pathology, Karolinska Institutet, Cancer Centrum Karolinska, Stockholm, Sweden, Jose Luis Venero, Departamento de Bioquimica y Biología, Instituto de Biomedicina de Sevilla, Hospital Universitario, Sevilla, Spain.".
- 2013940920 type "Laboratory Manuals. mesh".
- 2013940920 type "text".