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- 01H3M3TM0VYNQDV5582RQD5HBV classification A1.
- 01H3M3TM0VYNQDV5582RQD5HBV date "2023".
- 01H3M3TM0VYNQDV5582RQD5HBV language "eng".
- 01H3M3TM0VYNQDV5582RQD5HBV type journalArticle.
- 01H3M3TM0VYNQDV5582RQD5HBV hasPart 01H3M3W8TDJKAMGH91X77NZ80X.pdf.
- 01H3M3TM0VYNQDV5582RQD5HBV subject "Veterinary Sciences".
- 01H3M3TM0VYNQDV5582RQD5HBV doi "10.3390/vetsci10050331".
- 01H3M3TM0VYNQDV5582RQD5HBV issn "2306-7381".
- 01H3M3TM0VYNQDV5582RQD5HBV issue "5".
- 01H3M3TM0VYNQDV5582RQD5HBV volume "10".
- 01H3M3TM0VYNQDV5582RQD5HBV abstract "Simple SummaryThis preliminary study aimed to assess the presence of bacteria in pregnancy tissues belonging to healthy canine and feline feto-maternal units at term. Specifically, it included bitches and queens undergoing elective cesarean sections and their first extracted fetus. The placental side of the endometrium, amniotic fluid, and meconium were sampled as tissue representative of the intra-uterine environment during pregnancy. Sampling and laboratory protocols were elaborated to contrast the possibility for contamination and included strict selection criteria (only elective cesarean sections, no recent treatments with antimicrobials), sterility during sample collection, and sampling and laboratory controls. Samples were processed using both culture and molecular techniques (16S rRNA bacterial gene sequencing). When positive, culture revealed the presence of bacteria that are common contaminants and sequencing yielded a very low bacterial load. A difference was highlighted between canine and feline samples, suggesting a possible contamination from the skin of the dam, although the small sample size prevents any definitive conclusion. This study suggests that healthy canine and feline fetuses might develop in the presence of low amounts of bacterial components, although future research should include stricter protocols to check for contamination and provide information on bacterial viability.At present, there are no data on the presence of bacteria in healthy canine and feline pregnancies at term. Here, we investigated the uterine microbiome in bitches (n = 5) and queens (n = 3) undergoing elective cesarean section in two facilities. Samples included swabs from the endometrium, amniotic fluid, and meconium, and environmental swabs of the surgical tray as controls. Culture and 16S rRNA gene sequencing were used to investigate the presence of bacteria. Culture was positive for 34.3% of samples (uterus n = 3, amniotic fluid n = 2, meconium n = 4, controls n = 0), mostly with low growth of common contaminant bacteria. With sequencing techniques, the bacterial abundance was significantly lower than in environmental controls (p < 0.05). Sequencing results showed a species-specific pattern, and significant differences between canine and feline bacterial populations were found at order, family, and genus level. No differences were found in alpha and beta diversities between feto-maternal tissues and controls (p > 0.05). Dominant phyla were Bacteroidetes, Firmicutes, and Proteobacteria in different proportions based on tissue and species. Culture and sequencing results suggest that the bacterial biomass is very low in healthy canine and feline pregnancies at term, that bacteria likely originate from contamination from the dam's skin, and that the presence of viable bacteria could not be confirmed most of the time.".
- 01H3M3TM0VYNQDV5582RQD5HBV author F4F831BA-F0ED-11E1-A9DE-61C894A0A6B4.
- 01H3M3TM0VYNQDV5582RQD5HBV author bd52aff9-3383-11ec-881d-c14ba960acf2.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:32f2d669-f12a-44b5-a1f5-01ba8a832a5b.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:471197cf-65a2-43bd-8867-21389844a773.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:68477700-f699-4c66-92a7-62809d678e63.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:91a5e035-523a-4fe4-b021-4935a9cc2392.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:9fe7eae7-c4b9-4e5b-8a36-9142b13270fe.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:e44e9c5d-25fd-43d8-86c6-93a6a6150439.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:efc5cf83-9a96-4b05-9636-9b3438a575a2.
- 01H3M3TM0VYNQDV5582RQD5HBV author urn:uuid:f1db07e8-4f0e-4c35-bef7-5c69cda2a445.
- 01H3M3TM0VYNQDV5582RQD5HBV dateCreated "2023-06-23T12:31:52Z".
- 01H3M3TM0VYNQDV5582RQD5HBV dateModified "2024-12-12T17:50:30Z".
- 01H3M3TM0VYNQDV5582RQD5HBV name "Challenging the hypothesis of in utero microbiota acquisition in healthy canine and feline pregnancies at term : preliminary data".
- 01H3M3TM0VYNQDV5582RQD5HBV pagination urn:uuid:f52bed2b-93f5-43a0-be37-87179e3fa232.
- 01H3M3TM0VYNQDV5582RQD5HBV publisher urn:uuid:58d973f4-2488-4181-8634-82044447dc2e.
- 01H3M3TM0VYNQDV5582RQD5HBV sameAs LU-01H3M3TM0VYNQDV5582RQD5HBV.
- 01H3M3TM0VYNQDV5582RQD5HBV sourceOrganization urn:uuid:341f316e-5b3a-4160-bfcb-e1aade0da9f4.
- 01H3M3TM0VYNQDV5582RQD5HBV type A1.