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- 01HPH6AVTWFM4BK8WGKPJXR8AA classification A1.
- 01HPH6AVTWFM4BK8WGKPJXR8AA date "2024".
- 01HPH6AVTWFM4BK8WGKPJXR8AA language "eng".
- 01HPH6AVTWFM4BK8WGKPJXR8AA type journalArticle.
- 01HPH6AVTWFM4BK8WGKPJXR8AA hasPart 01HPH6C42TB159WJ50A69CZSN9.pdf.
- 01HPH6AVTWFM4BK8WGKPJXR8AA subject "Veterinary Sciences".
- 01HPH6AVTWFM4BK8WGKPJXR8AA doi "10.1016/j.theriogenology.2023.12.018".
- 01HPH6AVTWFM4BK8WGKPJXR8AA issn "0093-691X".
- 01HPH6AVTWFM4BK8WGKPJXR8AA issn "1879-3231".
- 01HPH6AVTWFM4BK8WGKPJXR8AA volume "216".
- 01HPH6AVTWFM4BK8WGKPJXR8AA abstract "The reproductive microbiota of male dogs has never been investigated using culture-independent sequencing techniques. The purpose of the present study was to get seminal knowledge on the microbiota of the ejaculate. Specifically, factors as the fraction of the ejaculate, the sperm quality (normospermia, teratozoospermia), and the living environment were evaluated. The sperm-rich and the prostatic fractions of the ejaculate were collected from healthy stud dogs. Following the sperm analysis, samples from twenty animals (normospermic n = 10 and teratozoospermic n = 10) were stored at - 80 C-degrees until further processing including DNA extraction and 16S rRNA sequencing. Alpha- (Shannon index) and beta- (Bray-Curtis, Unweighted UniFrac) diversities were assessed and compared (PERMANOVA) based on the group of samples (biological samples from the ejaculate and controls), the fraction of the ejaculate (sperm-rich and prostatic fractions), the animal group (normospermia and teratozoospermia), and the living environment of the animal (kennel or pet living in-house). The most abundant bacterial phyla in canine semen samples were Proteobacteria, Firmicutes, and Actinobacteria. Overall, the dominant bacterial family was that of Pasteurellaceae The genus Mycoplasma was never detected. No differences in terms of bacterial composition were found based on the fraction of the ejaculate and based on the animal group (P > 0.05). On the other hand, differences in alpha and beta diversities were highlighted based on the living environment (P = 0.001).Overall, the results of the present study provide preliminary insights on dog semen microbiota, opening a new chapter in the field of canine andrology. Our results suggest that the environment may play a role in influencing the reproductive microbiota of male dogs and that the prostatic fraction of the ejaculate can be used for further research as a representative of the semen microbiota.".
- 01HPH6AVTWFM4BK8WGKPJXR8AA author 02F2EEB8-F0EE-11E1-A9DE-61C894A0A6B4.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author 422D1088-B5D5-11E8-B8AA-A1D15607D3EF.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author CE71731C-8D05-11E7-B375-20EDAD28A064.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author D44FCAA2-62EE-11E9-A65B-09FA5607D3EF.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author F4F831BA-F0ED-11E1-A9DE-61C894A0A6B4.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author bd52aff9-3383-11ec-881d-c14ba960acf2.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author urn:uuid:6ffc963a-37ab-46b0-aac4-39e5a2885733.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author urn:uuid:9b48fba6-763f-4d27-836a-f248d33e4b79.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author urn:uuid:bb2b8391-bf17-4197-a548-274787aff468.
- 01HPH6AVTWFM4BK8WGKPJXR8AA author urn:uuid:d86152e1-04da-4d2c-984f-84ef211554c7.
- 01HPH6AVTWFM4BK8WGKPJXR8AA dateCreated "2024-02-13T12:16:13Z".
- 01HPH6AVTWFM4BK8WGKPJXR8AA dateModified "2024-12-12T20:17:17Z".
- 01HPH6AVTWFM4BK8WGKPJXR8AA name "Characterization of the semen microbiota of healthy stud dogs using 16S RNA sequencing".
- 01HPH6AVTWFM4BK8WGKPJXR8AA pagination urn:uuid:88a016a1-5b55-402d-a575-9a01ec8a07e3.
- 01HPH6AVTWFM4BK8WGKPJXR8AA publisher urn:uuid:ba722055-7b0a-47d2-bb5d-a2d46273c078.
- 01HPH6AVTWFM4BK8WGKPJXR8AA sameAs LU-01HPH6AVTWFM4BK8WGKPJXR8AA.
- 01HPH6AVTWFM4BK8WGKPJXR8AA sourceOrganization urn:uuid:641085f6-6317-4800-a687-110e00b226bd.
- 01HPH6AVTWFM4BK8WGKPJXR8AA type A1.