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- 01JA88NFBDTNRA3MX0B1H3H841 classification A1.
- 01JA88NFBDTNRA3MX0B1H3H841 date "2024".
- 01JA88NFBDTNRA3MX0B1H3H841 language "eng".
- 01JA88NFBDTNRA3MX0B1H3H841 type journalArticle.
- 01JA88NFBDTNRA3MX0B1H3H841 hasPart 01JAG091E04D3JPXT0FR1CMGDS.pdf.
- 01JA88NFBDTNRA3MX0B1H3H841 subject "Medicine and Health Sciences".
- 01JA88NFBDTNRA3MX0B1H3H841 subject "Veterinary Sciences".
- 01JA88NFBDTNRA3MX0B1H3H841 doi "10.1186/s40104-024-00997-7".
- 01JA88NFBDTNRA3MX0B1H3H841 issn "1674-9782".
- 01JA88NFBDTNRA3MX0B1H3H841 issn "2049-1891".
- 01JA88NFBDTNRA3MX0B1H3H841 issue "1".
- 01JA88NFBDTNRA3MX0B1H3H841 volume "15".
- 01JA88NFBDTNRA3MX0B1H3H841 abstract "Transfer RNA-derived small RNAs (tsRNAs) have been shown to be involved in early embryo development and repression of endogenous retroelements in embryos and stem cells. However, it is unknown whether tsRNAs also regulate embryo hatching. In this study, we mined the sequencing data of a previous experiment in which we demonstrated that the microRNA (miRNA) cargo of preimplantation embryonic extracellular vesicles (EVs) influences embryo development. We thus profiled the tsRNA cargo of EVs secreted by blastocysts and non-blastocysts. The majority of tsRNAs was identified as tRNA halves originating from the 5 ends of tRNAs. Among the 148 differentially expressed tsRNAs, the 19 nt tRNA fragment (tRF) tDR-14:32-Glu-CTC-1 was found to be significantly up-regulated in EVs derived from non-blastocysts. RT-qPCR assays confirmed its significant up-regulation in non-blastocyst embryos and their conditioned medium compared to the blastocyst group (P < 0.05). Inhibition of tDR-14:32-Glu-CTC-1 by supplementing antagomirs to the conditioned medium improved embryo hatching (P < 0.05). Transcriptomic analysis of embryos treated with tDR-14:32-Glu-CTC-1 antagomirs further showed differential expression of genes that are associated with embryo hatching and implantation. In summary, tDR-14:32-Glu-CTC-1 is up-regulated in non-blastocyst embryos and their secretions, and inhibition of tDR-14:32-Glu-CTC-1 promotes embryo hatching, while influencing embryo implantation-related genes and pathways. These results indicate that embryonic EVs containing specific tRFs may regulate preimplantation embryo development.".
- 01JA88NFBDTNRA3MX0B1H3H841 author 34efb4e2-8e87-11eb-8a1f-bb1c7cf690ae.
- 01JA88NFBDTNRA3MX0B1H3H841 author 5B305BB6-0FE8-11E6-A633-B0D3B4D1D7B1.
- 01JA88NFBDTNRA3MX0B1H3H841 author F469A738-F0ED-11E1-A9DE-61C894A0A6B4.
- 01JA88NFBDTNRA3MX0B1H3H841 author F4F831BA-F0ED-11E1-A9DE-61C894A0A6B4.
- 01JA88NFBDTNRA3MX0B1H3H841 author F86AD622-F0ED-11E1-A9DE-61C894A0A6B4.
- 01JA88NFBDTNRA3MX0B1H3H841 dateCreated "2024-10-15T14:17:44Z".
- 01JA88NFBDTNRA3MX0B1H3H841 dateModified "2024-12-12T21:05:45Z".
- 01JA88NFBDTNRA3MX0B1H3H841 name "tRNAGlu-derived fragments from embryonic extracellular vesicles modulate bovine embryo hatching".
- 01JA88NFBDTNRA3MX0B1H3H841 pagination urn:uuid:da8b5766-60af-4921-b4e2-8e9a61b4a634.
- 01JA88NFBDTNRA3MX0B1H3H841 publisher urn:uuid:79302710-2e57-4af0-89c1-64743de377db.
- 01JA88NFBDTNRA3MX0B1H3H841 sameAs LU-01JA88NFBDTNRA3MX0B1H3H841.
- 01JA88NFBDTNRA3MX0B1H3H841 sourceOrganization urn:uuid:79783741-874f-40da-9a42-6ad5000ab643.
- 01JA88NFBDTNRA3MX0B1H3H841 sourceOrganization urn:uuid:acbe1678-afec-4945-a077-653843342dfa.
- 01JA88NFBDTNRA3MX0B1H3H841 sourceOrganization urn:uuid:eed24ed6-5d19-488a-9bb2-8ceada83227c.
- 01JA88NFBDTNRA3MX0B1H3H841 type A1.