Matches in UGent Biblio for { <https://biblio.ugent.be/publication/1009957#aggregation> ?p ?o. }
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- aggregation classification "C3".
- aggregation creator B83850.
- aggregation creator B83851.
- aggregation creator B83852.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2010".
- aggregation hasFormat 1009957.bibtex.
- aggregation hasFormat 1009957.csv.
- aggregation hasFormat 1009957.dc.
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- aggregation hasFormat 1009957.doc.
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- aggregation isPartOf urn:isbn:9789058642264.
- aggregation language "eng".
- aggregation publisher "Ghent University".
- aggregation subject "Veterinary Sciences".
- aggregation title "Insulin like growth factor-I in heat stressed bovine oocytes: effect on embryo development".
- aggregation abstract "The aim of the present study was to investigate the effects of insulin like growth factor-I (IGF-I) during in vitro maturation of bovine oocytes under heat stress (HS) on subsequent cleavage rate (CR) at 45 hours post insemination (hpi), blastocyst rate (BR) at 168 hpi and apoptotic cell ratio (ACR) after active caspase staining at both time points. Selected oocytes were assigned to four maturation groups in serum-free media supplemented with EGF (20 ng/ml) as follows, two control groups: control (maturation temperature 38.5°C for 22h) or HS-control (maturation temperature 40.5°C for 12h followed by 38.5°C for 10h) and two treatment groups with the addition of IGF-I (100 ng/ml); IGF-I (maturation temperature 38.5°C) or HS-IGF-I (maturation temperature 40.5°C for 12h followed by 38.5°C for 10h). Matured oocytes were fertilized and cultured in SOF medium supplemented with 5 % FCS. Half of the cultured zygotes were checked at 45 hpi for their CR and ACR, while the other half was checked at 168 hpi for determining BR and ACR. General linear models (CR and BR) or logistic models (ACR) were used to analyze the data. The results of CR were 82.8±1a, 53.1±1.5bc, 73.9±1.1ab and 45.5±1.8c % and the BR were 31.7±5.8a, 10.2±1.6b, 26.4±4.8a and 11.9±2.2b %, in IGF-I, HS-IGF-I, control and HS-control groups, respectively (a,b,c P<0.05). The results of ACR at 45 hpi were 16.5±2.2a, 17±4.2a, 19.7±3.8 a and 23.2±4.6b % and ACR results at 168 hpi were 3.1±0.8a, 7.8±2.1bc, 5±0.6b and 7±2.1c %, for the IGF-I, HS-IGF-I, control and HS-control groups, respectively (a,b,c P<0.05). It can be concluded that HS reduced both CR and BR and increased ACR either at 45 or 168 hpi. Addition of IGF-I during maturation under HS conditions rescued cleaved zygotes from apoptosis and tended to improve CR (P=0.06), however, no beneficial effects could be detected at 168 hpi either on BR or ACR, which may indicate that IGF-I added during maturation under HS could rescue preimplantation embryos in early stages of development but this effect was not preserved after embryonic genome activation.".
- aggregation authorList BK215627.
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- aggregation similarTo LU-1009957.