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- aggregation classification "C3".
- aggregation creator B77953.
- aggregation creator B77954.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation creator person.
- aggregation date "2010".
- aggregation hasFormat 1018505.bibtex.
- aggregation hasFormat 1018505.csv.
- aggregation hasFormat 1018505.dc.
- aggregation hasFormat 1018505.didl.
- aggregation hasFormat 1018505.doc.
- aggregation hasFormat 1018505.json.
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- aggregation hasFormat 1018505.txt.
- aggregation hasFormat 1018505.xls.
- aggregation hasFormat 1018505.yaml.
- aggregation isPartOf urn:issn:0936-6768.
- aggregation language "eng".
- aggregation subject "Veterinary Sciences".
- aggregation title "Addition of insulin like growth factor-1 during maturation and culture enhances blastocyst rate and reduces apoptosis in bovine blastocysts derived from heat stressed oocytes".
- aggregation abstract "Heat stress (HS) reduces bovine oocyte developmental competence. Previous research showed that the addition of insulin like growth factor-1 (IGF-1) during maturation reduced apoptosis in heat stressed oocytes with no apparent effect on blastocyst rates. We hypothesized that adding IGF-1 during maturation and culture may enhance blastocyst rate and decrease apoptosis in blastocysts derived from heat stressed oocytes. During the first 12 h of maturation, HS (40.5C) was applied while IGF-1 (100 ng/ml) was added during maturation (M), during culture (C) or during both (MC). Two control groups were included (negative-control: no HS nor IGF-1 and HS-control: HS but no IGF-1). Blastocyst rates were 27.9 ± 2.1a, 10.9 ± 1.2b, 9.7 ± 1.2b, 18.4 ± 2.4c, and 15.3 ± 1.9bc % in negative-control, HS- ontrol, M, C and MC groups, respectively (a,b,c p < 0.05). Apoptotic cell ratios (ACR) were 9.7 ± 1a, 11.8 ± 1.2a, 11.6 ± 2a, 7.8 ± 0.8b and 5.9 ± 0.7c % in negative-control, HS-control, M, C and MC groups, respectively (a,b,c p < 0.05). The addition of IGF-1 to the culture medium increased blastocyst rate in comparison to HS-control group. However, IGF-1 had more beneficial effects on ACR when added during both maturation and culture than during maturation or culture only. In conclusion, negative effects of HS on oocyte developmental competence can be reduced by adding IGF-1 during both maturation and culture.".
- aggregation authorList BK199703.
- aggregation endPage "71".
- aggregation issue "suppl. 3".
- aggregation startPage "71".
- aggregation volume "45".
- aggregation isDescribedBy 1018505.
- aggregation similarTo j.1439-0531.2010.01654_3.x.
- aggregation similarTo LU-1018505.