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• 01GSWSQB51S9FT9M3EYZ6NB156 abstract "BackgroundPublic health and clinical recommendations are established from systematic reviews and retrospective meta-analyses combining effect sizes, traditionally, from aggregate data and more recently, using individual participant data (IPD) of published studies. However, trials often have outcomes and other meta-data that are not defined and collected in a standardized way, making meta-analysis problematic. IPD meta-analysis can only partially fix the limitations of traditional, retrospective, aggregate meta-analysis; prospective meta-analysis further reduces the problems.MethodsWe developed an initiative including seven clinical intervention studies of balanced energy-protein (BEP) supplementation during pregnancy and/or lactation that are being conducted (or recently concluded) in Burkina Faso, Ethiopia, India, Nepal, and Pakistan to test the effect of BEP on infant and maternal outcomes. These studies were commissioned after an expert consultation that designed recommendations for a BEP product for use among pregnant and lactating women in low- and middle-income countries. The initiative goal is to harmonize variables across studies to facilitate IPD meta-analyses on closely aligned data, commonly called prospective meta-analysis. Our objective here is to describe the process of harmonizing variable definitions and prioritizing research questions. A two-day workshop of investigators, content experts, and advisors was held in February 2020 and harmonization activities continued thereafter. Efforts included a range of activities from examining protocols and data collection plans to discussing best practices within field constraints. Prior to harmonization, there were many similar outcomes and variables across studies, such as newborn anthropometry, gestational age, and stillbirth, however, definitions and protocols differed. As well, some measurements were being conducted in several but not all studies, such as food insecurity. Through the harmonization process, we came to consensus on important shared variables, particularly outcomes, added new measurements, and improved protocols across studies.DiscussionWe have fostered extensive communication between investigators from different studies, and importantly, created a large set of harmonized variable definitions within a prospective meta-analysis framework. We expect this initiative will improve reporting within each study in addition to providing opportunities for a series of IPD meta-analyses.".
• 01GSWV37J4D100PBQMXSDRXRBT abstract "Background Latent growth curve modeling was used to investigate the longitudinal link between attachment, effortful control (EC), and maladaptive development during middle childhood. Methods In a community sample, children (Time 1: n = 157; M-age = 10.91) and their mothers were examined three times over a two-year period. Attachment was operationalized at a more strategic (self-reported trust in maternal support) and more automatic level (secure base script knowledge). Mothers reported about children's EC and maladjustment. Results Secure attachment was associated with higher EC, but EC development was only linked with baseline self-reported trust. Also, EC indirectly linked baseline self-reported trust with change in externalizing and internalizing problems over time. In addition, self-reported trust was indirectly linked with change in externalizing problems over time through EC development. Conclusion EC, and, less robustly, EC development were linked with attachment and change in emotional and behavioral problems.".
• 01GSWWM6ASWDAWZVBVKFCNSSHN abstract "Recombinant proteins (RP) are widely used as biopharmaceuticals, industrial enzymes, or sustainable food source. Yeasts, with their ability to produce complex proteins through a broad variety of cheap carbon sources, have emerged as promising eukaryotic production hosts. As such, the prevalence of yeasts as favourable pro-duction organisms in commercial RP production is expected to increase. Yet, with the selection of a robust production host on the one hand, successful scale-up is dependent on a thorough understanding of the chal-lenging environment and limitations of large-scale bioreactors on the other hand. In the present work, several prominent yeast species, including Saccharomyces cerevisiae, Pichia pastoris, Yarrowia lipolytica, Kluyveromyces lactis and Kluyveromyces marxianus are reviewed for their current state and performance in commercial RP production. Thereafter, the impact of principal process control parameters, including dissolved oxygen, pH, substrate concentration, and temperature, on large-scale RP production are discussed. Finally, technical chal-lenges of process scale-up are identified. To that end, process intensification strategies to enhance industrial feasibility are summarized, specifically highlighting fermentation strategies to ensure sufficient cooling capacity, overcome oxygen limitation, and increase protein quality and productivity. As such, this review aims to contribute to the pursuit of sustainable yeast-based RP production.".
• 01GSWYP4S7X5TDAFGQE1WMYJT7 abstract "Background and purpose: To establish an international quality standard for contouring and planning for high-risk neuroblastoma within the prospective High-Risk Neuroblastoma Study 2 of SIOP-Europe-Neuroblastoma (SIOPEN HR-NBL2), which includes a randomized question on dose escalation for residual disease. Materials and methods: Data on four patients with high-risk neuroblastoma were selected and distributed to the radiotherapy committee of the HR-NBL2 study for independent contouring and planning. Differences in contouring were analyzed using apparent and kappa-corrected agreement. Plans were ana-lyzed regarding the dose-volume histogram metrics. Results were discussed among experts and agree-ment was obtained.Results: Substantial agreement was found for contouring of the heart (0.64), liver (0.70), left lung (0.74), and right lung (0.74). For contouring of the gastrointestinal tract (0.54), left kidney (0.60), and right kid-ney (0.59) moderate agreement was obtained. For target volume delineation, agreement for preoperative tumour extent was moderate (0.42), for CTV fair (0.35) and only low (0.06) for residual tumour, respec-tively. The dose planning strategies appeared to be relatively homogeneous among all experts.Conclusion: Considerable variability was found for the delineation of target volumes, particularly the boost volume, whereas the contouring of the organs at risk and the planning strategy were reasonably consistent. In order to obtain reliable results from the randomized HR-NBL2 trial, standardization of tar-get volume delineation based on adequate imaging is crucial.(c) 2023 Published by Elsevier B.V. Radiotherapy and Oncology 181 (2023) 109464".
• 01GSXE1ZAQNDPYYRGF87GK3XN5 abstract "Food waste, especially at the retail and consumer level, is a critical societal issue. Consumers' reluctance to purchase and consume near-expired food is a major contributor. Retailers have taken actions to promote near-expired food; however, it is unclear how their actions influence both purchase and consumption of near-expired food. This research examines one retail strategy aiming to reduce food waste-a message about food waste avoidance (without discounts). Specifically, four experiments (N = 1196) using various measurements of food waste prevention behaviors and one single-paper meta-analysis reveal that a message about food waste avoidance increases consumers' willingness to buy near-expired food through increased moral satisfaction. After purchasing near-expired food, consumers engage in more waste prevention behaviors for it than for other food regardless of whether consumers encounter the food-waste-avoidance message. In addition, we find indications that increased moral satisfaction deriving from the food-waste-avoidance message motivates consumers to conduct more household waste prevention behaviors for the purchased near-expired food. Together, these findings suggest that a message about food waste avoidance can be a potentially effective strategy to reduce the waste of near-expired food. This research extends our understanding of the purchase and consumption of near-expired food and supports retail messages about food waste avoidance to sell near-expired food.".
• 01GSXE1ZB4MQ13494E8MP7RZ4Q abstract "One core fundamental need that people may try to address through consumption is that of affiliation, or the need to belong. First, giving gifts may serve that goal, but it may also potentially hurt the formation of affiliative bonds if it creates a sense of indebtedness. Second, experiential consumption serves the need to belong if it is enjoyed socially. Third, symbolic consumption may help craft a prosocial image, but may also hinder it if one engages in conspicuous consumption to demonstrate one's wealth. Finally, consumption of media that allows the formation of parasocial relations with influencers and celebrities, and presence and activity in online communities can act as a surrogate for offline satisfaction of the need to belong.".
• 01GSXE1ZB8FQQB714HQ7BJYYZ2 abstract "Five experiments (total n = 2422, with U.S. American and French participants, four preregistered) show that people are more likely to use median salaries rather than CEO-median employee compensation ratios when making inequality and fairness judgments based on company compensation data. In separate evaluation of companies, we find no significant impact of compensation ratios, which express objective levels of income inequality, but a significant impact of median salaries. In joint evaluation, ratios have an impact, but median salaries have a bigger impact. Our results point to a difference between perceived and actual inequality indicators: people do not perceive inequality based on a widely-used indicator of inequality (compensation ratios), but rather use representative workers' salaries, and believe lower representative wages are connected to higher inequality. We discuss theoretical implications for the psychological understanding of economic inequality, and practical implications for the regulation of the presentation of compensation data.".
• 01GSXE1ZBCK7MFK55BKWM8D1SH abstract "Firms pursuing expansion abroad increasingly face challenges of protectionism and discrimination against foreign products, a phenomenon widely recognized as domestic country bias. This research addresses discordant findings in previous work by introducing a new mechanism of domestic country bias that operates distinctly for national identifiers and ethnocentric consumers, connecting these two groups to regulatory focus theory. Using three experimental studies and a survey involving actual product possessions, we provide new evidence that consistently demonstrates that national identity and consumer ethnocentrism are associated with different goals, namely, an approach goal and an avoidance goal, respectively. Importantly, the results reveal that domestic country bias due to national identity can be attenuated by priming a prevention focus, while domestic country bias due to consumer ethnocentrism can be reduced by priming a promotion focus. The findings offer international marketing managers valuable insights into reducing domestic country bias and effectively segmenting international consumer markets. This research is the first to demonstrate how global companies can actively overcome domestic country bias by deploying suitable international marketing programs rather than avoiding specific segments and/or downplaying foreign origins.".
• 01GSXE1ZBGRWPHFJ0FN5FWKJCK abstract "We report a consistent effect in the evaluation of actions: later actions - specifically, actions that are closer to a final, positive outcome - are considered as contributing more to that outcome, compared to earlier actions. Twelve experiments (total n = 5658, six pre-registered, with U.S. American and British participants, manipulating action timing both within-subjects and between-subjects) provide evidence in support of a late-action effect. This effect extends to different domains, from sports to business to academics. Further, this effect is disproportionately larger when actions are closer to the end of an event. We identify two mediators. The first is a heightened tendency to imagine negative alternative scenarios when the action is closer to an outcome, that is, higher counterfactual potency for later actions. The second is perceived outcome reversibility - lower for later actions, when there is less time for the provisional outcome to change. The effect is mitigated if people are instructed to imagine counterfactual outcomes (consistent with the notion of a counterfactual potency mediation), and if the action does not produce a provisional outcome change. We discuss theoretical implications for the psychological understanding of timing, causality attributions, and counterfactual thinking, as well as future research directions for goal-gradient (the tendency to give more weight to the last steps towards goal completion).".
• 01GSXE1ZBKSY8QZ8P30H0TJ9NK abstract "This research documents a "light = healthy" intuition, such that consumers perceive foods that weigh less are healthier than their heavier counterparts with the same serving size. Subsequently, consumers consume a larger quantity of lighter-weight foods. The intuition is based on a coactivation of two meanings of the word "light": light in physical weight and light in calorie content. An implicit attitude test finds support for this association between physical weight and food healthiness. Subsequently, physically lighter foods are perceived to be healthier because they are assumed to contain fewer calories. In line with the proposed coactivation mechanism, the intuition is bidirectional, where consumers also expect healthier foods to weigh less. Consequently, they discredit health claims issued for heavier foods. Finally, it was found that activating a competing intuition is effective at debiasing the "light = healthy" intuition.".
• 01GSXE1ZBQ2YB41BDB95N9VXT4 abstract "Prior research has documented a denomination effect, such that consumers are less willing to spend banknotes in large denominations (e.g., $100 bill) than in small denominations (e.g., five $20 bills). This research shows that the denomination effect persists when prices are low, but when prices are high, a reverse denomination effect applies, such that consumers prefer to pay for expensive products with large denominations. To accommodate both effects in a general framework, the authors propose a denomination-spending matching effect: consumers prefer to pay with a denomination that matches the spending amount. This matching effect is generalizable to other payment means (i.e., gift cards). A denomination fit underlies this matching effect-when denomination and spending have a similar magnitude, consumers feel right about the purchase. This experience of denomination fit prompts consumers to choose products where the price matches the denomination and increases consumers' purchase satisfaction and anticipated consumption experience.".
• 01GSXE1ZBTE24FXV5VRS3WCJCX abstract "Prior research found that glossy objects not only excel in drawing attention but also are more positively evaluated than matte ones. Contrary to that research, we show that glossy packaging may adversely affect brand trust. This negative effect of glossy packaging appears robust across different contexts and product categories. We find that consumers evaluate a product in a glossy package as less trustworthy because they view its use as a desperate attempt to draw consumers' attention. Their negative reaction to glossy packaging thus forms part of their persuasion knowledge.".
• 01GSYF30CAFBJQB0BXPBRRDVA4 abstract "Background Digital health interventions have shown promising results for the management of type 2 diabetes, but a comparison of the effectiveness and implementation of the different modes is not currently available. Therefore, this study aimed to compare the effectiveness of SMS, smartphone application, and website-based interventions on improving glycaemia in adults with type 2 diabetes and report on their reach, uptake, and feasibility. Methods In this systematic review and meta-analysis, we searched CINAHL, Cochrane Central, Embase, MEDLINE, and PsycInfo on May 25, 2022, for randomised controlled trials (RCTs) that examined the effectiveness of digital health interventions in reducing glycated haemoglobin A1c (HbA1c) in adults with type 2 diabetes, published in English from Jan 1, 2009. Screening was carried out using Covidence, and data were extracted following Cochrane's guidelines. The primary endpoint assessed was the change in the mean (and 95% CI) plasma concentration of HbA1c at 3 months or more. Cochrane risk of bias 2 was used to assess risk of bias. Data on reach, uptake, and feasibility were summarised narratively and data on HbA1c reduction were synthesised in a meta-analysis. Grading of Recommendations, Assessment, Development, and Evaluation criteria was used to evaluate the level of evidence. The study was registered with PROSPERO, CRD42021247845. Findings Of the 3236 records identified, 56 RCTs from 24 regions (n=11 486 participants), were included in the narrative synthesis, and 26 studies (n=4546 participants) in the meta-analysis. 20 studies used SMS as the primary mode of delivery of the digital health intervention, 25 used smartphone applications, and 11 implemented interventions via websites. Smartphone application interventions reported higher reach compared with SMS and website-based interventions, but website-based interventions reported higher uptake compared with SMS and smartphone application interventions. Effective interventions, in general, included people with greater severity of their condition at baseline (ie, higher HbA1c) and administration of a higher dose intensity of the intervention, such as more frequent use of smartphone applications. Overall, digital health intervention group participants had a –0·30 (95% CI –0·42 to –0·19) percentage point greater reduction in HbA1c, compared with control group participants. The difference in HbA1c reduction between groups was statistically significant when interventions were delivered through smartphone applications (–0·42% [–0·63 to –0·20]) and via SMS (–0·37% [–0·57 to –0·17]), but not when delivered via websites (–0·09% [–0·64 to 0·46]). Due to the considerable heterogeneity between included studies, the level of evidence was moderate overall. Interpretation Smartphone application and SMS interventions, but not website-based interventions, were associated with better glycaemic control. However, the studies' heterogeneity should be recognised. Considering that both smartphone application and SMS interventions are effective for diabetes management, clinicians should consider factors such as reach, uptake, patient preference, and context of the intervention when deciding on the mode of delivery of the intervention. Nine in ten people worldwide own a feature phone and can receive SMS and four in five people have access to a smartphone, with numerous smartphone applications being available for diabetes management. Clinicians should familiarise themselves with this modality of programme delivery and encourage people with type 2 diabetes to use evidence-based applications for improving their self-management of diabetes. Future research needs to describe in detail the mediators and moderators of the effectiveness and implementation of SMS and smartphone application interventions, such as the optimal dose, frequency, timing, user interface, and communication mode to both further improve their effectiveness and to increase their reach, uptake, and feasibility.".
• 01GSYFJVJRJYTD9A9AN26YV8KG abstract "Background Digital health interventions are effective for hypertension self-management, but a comparison of the effectiveness and implementation of the different modes of interventions is not currently available. This study aimed to compare the effectiveness of SMS, smartphone application, and website interventions on improving blood pressure in adults with hypertension, and to report on their reach, uptake, and feasibility. Methods In this systematic review and meta-analysis we searched CINAHL Complete, Cochrane Central Register of Controlled Trials, Ovid Embase, Ovid MEDLINE, and APA PsycInfo on May 25, 2022, for randomised controlled trials (RCTs) published in English from Jan 1, 2009, that examined the effectiveness of digital health interventions on reducing blood pressure in adults with hypertension. Screening was carried out using Covidence, and data were extracted following Cochrane's guidelines. The primary endpoint was change in the mean of systolic blood pressure. Risk of bias was assessed with Cochrane Risk of Bias 2. Data on systolic and diastolic blood pressure reduction were synthesised in a meta-analysis, and data on reach, uptake and feasibility were summarised narratively. Grading of Recommendations, Assessment, Development, and Evaluation criteria were used to evaluate the level of evidence. The study was registered with PROSPERO CRD42021247845. Findings Of the 3235 records identified, 29 RCTs from 13 regions (n=7592 participants) were included in the systematic review, and 28 of these RCTs (n=7092 participants) were included in the meta-analysis. 11 studies used SMS as the primary mode of delivery of the digital health intervention, 13 used smartphone applications, and five used websites. Overall, digital health intervention group participants had a –3·62 mm Hg (95% CI –5·22 to –2·02) greater reduction in systolic blood pressure, and a –2·45 mm Hg (–3·83 to –1·07) greater reduction in diastolic blood pressure, compared with control group participants. No statistically significant differences between the three different modes of delivery were observed for both the systolic (p=0·73) and the diastolic blood pressure (p=0·80) outcomes. Smartphone application interventions had a statistically significant reduction in diastolic blood pressure (–2·45 mm Hg [–4·15 to –0·74]); however, there were no statistically significant reductions for SMS interventions (–1·80 mm Hg [–4·60 to 1·00]) or website interventions (–3·43 mm Hg [–7·24 to 0·38]). Due to the considerable heterogeneity between included studies and the high risk of bias in some, the level of evidence was assigned a low overall score. Interventions were more effective among people with greater severity of hypertension at baseline. SMS interventions reported higher reach and smartphone application studies reported higher uptake, but differences were not statistically significant. Interpretation SMS, smartphone application, and website interventions were associated with statistically and clinically significant systolic and diastolic blood pressure reductions, compared with usual care, regardless of the mode of delivery of the intervention. This conclusion is tempered by the considerable heterogeneity of included studies and the high risk of bias in most. Future studies need to describe in detail the mediators and moderators of the effectiveness and implementation of these interventions, to both further improve their effectiveness as well as increase their reach, uptake, and feasibility.".
• 01GSYMG67Z4501FKGQ3W7BJJAW abstract "While metal halide perovskites (MHPs) have shown great potential for various optoelectronic applications, their widespread adoption in commercial photovoltaic cells or photo-sensors is currently restricted, given that MHPs such as CsPbI3 and FAPbI(3) spontaneously transition to an optically inactive non-perovskite phase at ambient conditions. Herein, we put forward an accurate first-principles procedure to obtain fundamental insight into this phase stability conundrum. To this end, we computationally predict the Helmholtz free energy, composed of the electronic ground state energy and thermal corrections, as this is the fundamental quantity describing the phase stability in polymorphic materials. By adopting the random phase approximation method as a wave function-based method that intrinsically accounts for many-body electron correlation effects as a benchmark for the ground state energy, we validate the performance of different exchange-correlation functionals and dispersion methods. The thermal corrections, accessed through the vibrational density of states, are accessed through molecular dynamics simulations, using a phase-transferable machine learning potential to accurately account for the MHPs' anharmonicity and mitigate size effects. The here proposed procedure is critically validated on CsPbI3, which is a challenging material as its phase stability changes slowly with varying temperature. We demonstrate that our procedure is essential to reproduce the experimental transition temperature, as choosing an inadequate functional can easily miss the transition temperature by more than 100 K. These results demonstrate that the here validated methodology is ideally suited to understand how factors such as strain engineering, surface functionalization, or compositional engineering could help to phase-stabilize MHPs for targeted applications.".
• 01GSYMZ74YX7WN7SC94PSEQ346 abstract "Mixed-matrix membranes (MMMs) have been investigated to render energy-intensive separations more efficiently by combining the selectivity and permeability performance, robustness, and nonaging properties of the filler with the easy processing, handling, and scaling up of the polymer. However, truly combining all in one single material has proven very challenging. In this work, we filled a commercial polyimide with ultrahigh loadings of a high-aspect ratio, CO2-philic Na-SSZ-39 zeolite with a three-dimensional channel system that precisely separates gas molecules. By carefully designing both zeolite and MMM synthesis, we created a gas -percolation highway across a flexible and aging-resistant (more than 1 year) membrane. The combination of a CO2-CH4 mixed-gas selectivity of-423 and a CO2 permeability of-8300 Barrer outperformed all existing polymer-based membranes and even most zeolite-only membranes.".
• 01GSYNMHEZB1TMQNBSTPR23YGN abstract "Computational modeling of physical processes in metal-organic frameworks (MOFs) is highly challenging due to the presence of spatial heterogeneities and complex operating conditions which affect their behavior. Density functional theory (DFT) may describe interatomic interactions at the quantum mechanical level, but is computationally too expensive for systems beyond the nanometer and picosecond range. Herein, we propose an incremental learning scheme to construct accurate and data-efficient machine learning potentials for MOFs. The scheme builds on the power of equivariant neural network potentials in combination with parallelized enhanced sampling and on-the-fly training to simultaneously explore and learn the phase space in an iterative manner. With only a few hundred single-point DFT evaluations per material, accurate and transferable potentials are obtained, even for flexible frameworks with multiple structurally different phases. The incremental learning scheme is universally applicable and may pave the way to model framework materials in larger spatiotemporal windows with higher accuracy.".
• 01GSYPSR91VWK899Z2VWZ1MZGT abstract "Purpose: The clinical use of event-related potentials in patients with language disor-ders is increasingly acknowledged. For this purpose, normative data should be avail-able. Within this context, healthy aging and gender effects on the electrophysiologi-cal correlates of semantic sentence comprehension were investigated.Method: One hundred and ten healthy subjects (55 men and 55 women), divided among three age groups (young, middle aged, and elderly), performed a semantic sen-tence congruity task in the visual modality during electroencephalographic recording.Results: The early visual complex was affected by increasing age as shown by smaller P2 amplitudes in the elderly compared to the young. Moreover, the N400 effect in the elderly was smaller than in the young and was delayed com-pared to latency measures in both middle-aged and young subjects. The topog-raphy of age-related amplitude changes of the N400 effect appeared to be gen-der specific. The late positive complex effect was increased at frontal electrode sites from middle age on, but this was not statistically significant. No gender effects were detected regarding the early P1, N1, and P2, or the late positive complex effect.Conclusion: Especially aging effects were found during semantic sentence comprehension, and this from the level of perceptual processing on. Normative data are now available for clinical use.".
• 01GSYQ9Q38HWFZ2Y0CQ57G1BXA abstract "Electroencephalographic (EEG) and magnetoencephalographic (MEG) recordings during language processing can provide relevant insights on neuroplasticity in clinical populations (including patients with aphasia). To use EEG and MEG in a longitudinal way, the outcome measures should be consistent across time in healthy individuals. Therefore, the current study provides a review on the test-retest reliability of EEG and MEG measures elicited during language paradigms in healthy adults. PubMed, Web of Science and Embase were searched for relevant articles based on specific eligibility criteria. In total, 11 articles were included in this literature review. The test-retest reliability of the P1, N1 and P2 is systematically considered to be satisfactory, whereas findings are more variable for event-related potentials/fields occurring later in time. The within subject consistency of EEG and MEG measures during language processing can be influenced by multiple variables such as the stimulus presentation mode, the offline reference choice and the required amount of cognitive resources during the task. To conclude, most of the available results are favourable regarding the longitudinal use of EEG and MEG measures elicited during language paradigms in healthy young individuals. In view to the use of these techniques in patients with aphasia, future research should focus on whether the same findings apply to different age groups.".
• 01GSYQF21ADV15FXPVQ9MR2794 abstract "Clinical trials frequently include multiple end points that mature at different times. The initial report, typically based on the primary end point, may be published when key planned co-primary or secondary analyses are not yet available. Clinical Trial Updates provide an opportunity to disseminate additional results from studies, published in JCO or elsewhere, for which the primary end point has already been reported.In the primary analysis of the global phase II ELIANA trial (ClinicalTrials.gov identifier: ), tisagenlecleucel provided an overall remission rate of 81% in pediatric and young adult patients with relapsed or refractory B-cell acute lymphoblastic leukemia (R/R B-ALL), with 59% of responders remaining relapse-free at 12 months. Here, we report an update on efficacy, safety, and patient-reported quality of life in 79 pediatric and young adult patients with R/R B-ALL following a median follow-up of 38.8 months. The overall remission rate was 82%. The median event-free survival was 24 months, and the median overall survival was not reached. Event-free survival was 44% (95% CI, 31 to 57) and overall survival was 63% (95% CI, 51 to 73) at 3 years overall (most events occur within the first 2 years). The estimated 3-year relapse-free survival with and without censoring for subsequent therapy was 52% (95% CI, 37 to 66) and 48% (95% CI, 34 to 60), respectively. No new or unexpected long-term adverse events were reported. Grade 3/4 adverse events were reported in 29% of patients > 1 year after infusion; grade 3/4 infection rate did not increase > 1 year after infusion. Patients reported improvements in quality of life up to 36 months after infusion. These findings demonstrate favorable long-term safety and suggest tisagenlecleucel as a curative treatment option for heavily pretreated pediatric and young adult patients with R/R B-ALL.".
• 01GSYQRFAHPSHG56B50KGW6Y66 abstract "Life cycle assessment (LCA) was applied to evaluate black soldier fly production using different diets, including typical Belgian agro-residues (Brussels sprout stems, endive roots and solid fraction pig manure). The LCA compared insect-based feed with soybean meal and fishmeal, and composting through insects versus conventional treatments. Underlying LCA data were derived through feeding experiments. To determine the sensitivity of the results, we tested the effect of alternative energy sources and dietary components. Non-residue insect feed and energy use contributed greatly to overall environmental impacts. Insect protein had greater impacts than protein from soybean meal or fishmeal due to the high energy consumption and, in some cases, agro-product demands. These should be areas of focus to make European insect production more sustainable. In the case of Brussels sprout stems and endive roots, conventional treatments outperformed composting by insects. Between industrial versus insect pig manure composting, the results varied greatly by energy source and impact category.".
• 01GSYQTCYK15EV4QW45SX9JP89 abstract "Chimeric antigen receptor T cells targeting CD19 (CART-19) have shown remarkable efficacy for relapsed/refractory (R/R) B-cell precursor acute lymphoblastic leukemia (BCP-ALL). We investigated whether prior use of inotuzumab ozogamicin (InO), an anti-CD22 antibody conjugated to calicheamicin, may impact CAR T-cell manufacturing or efficacy via pre-CART-19 depletion of the B-cell compartment. In this international, retrospective analysis, 39 children and young adults receiving InO before (n = 12) and/or after (n = 27) T-cell apheresis as bridging therapy to CART-19 treatment were analyzed. Median age at infusion was 13 years (range 1.4-23 years). Thirty-four out of 39 patients (87.2%) obtained complete remission. With a median follow-up of 18.2 months after CART-19 infusion, 12-month event-free survival (EFS) was 53.3% (95% confidence interval (CI): 38.7-73.4) and overall survival (OS) was 77.8% (95% CI: 64.5-93.9). Seventeen patients (44%) relapsed with a median of 159 days (range 28-655) after CART-19 infusion. No difference in day 28 minimal residual disease negative complete response rate, 12-month OS/EFS, or incidence of CD19-positive or -negative relapses was observed among patients receiving InO before or after apheresis. Compared to published data for patients treated with CART-19 therapy without prior InO exposure, response and OS/EFS for patients treated with InO prior to CART-19 are similar.".
• 01GSYR9YGEACN1GCXQE1BT2625 abstract "High-throughput sequencing (HTS) of the immunoglobulin heavy chain (IgH) locus is a recent very efficient technique to monitor minimal residual disease of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). It also reveals the sequences of clonal rearrangements, therefore, the multiclonal structure, of BCP-ALL. In this study, we performed IgH HTS on the diagnostic bone marrow of 105 children treated between 2004 and 2008 in Belgium for BCP-ALL in the European Organization for Research and Treatment of Cancer (EORTC)-58951 clinical trial. Patients were included irrespectively of their outcome. We described the patterns of clonal complexity at diagnosis and investigated its association with patients' characteristics. Two indicators of clonal complexity were used, namely, the number of foster clones, described as clones with similar D-N-2-J rearrangements but other V-rearrangement and N-1-joining, and the maximum across all foster clones of the number of evolved clones from one foster clone. The maximum number of evolved clones was significantly higher in patients with t(12;21)/ETV6:RUNX1. A lower number of foster clones was associated with a higher risk group after prephase and t(12;21)/ETV6:RUNX1 genetic type. This study observes that clonal complexity as accessed by IgH HTS is linked to prognostic factors in childhood BCP-ALL, suggesting that it may be a useful diagnostic tool for BCP-ALL status and prognosis.".
• 01GSYRT3CCE9Q2WFD2GQC7SXTT abstract "A significant proportion of events in paediatric acute myeloid leukaemia (AML) are caused by resistant disease (RD). We investigated clinical and biological characteristics in 66 patients with RD from 1013 children with AML registered and treated according to the NOPHO-AML 93, NOPHO-AML 2004, DB AML-01 and NOPHO-DBH AML 2012 protocols. Risk factors for RD were age10 years or older and a white-blood-cell count (WBC) of 100 x 10(9)/L or more at diagnosis. The five-year overall survival (OS) was 38% (95% confidence interval [CI]: 28%-52%). Of the 63 children that received salvage therapy with chemotherapy, 59% (N = 37) achieved complete remission (CR) with OS 57% (95% CI: 42%-75%) compared to 12% (95% CI: 4%-35%) for children that did not achieve CR. Giving more than two salvage chemotherapy courses did not increase CR rates. OS for all 43 patients receiving allogeneic haematopoietic stem cell transplantation (HSCT) was 49% (95% CI: 36%-66%). Those achieving CR and proceeding to HSCT had an OS of 56% (95% CI: 41%-77%, N = 30). This study showed that almost 40% of children with primary resistant AML can be cured with salvage therapy followed by HSCT. Children that did not achieve CR after two salvage courses with chemotherapy did not benefit from additional chemotherapy.".
• 01GSYS28TQXMHYTKWHPDPRH0AP abstract "Treatment of chronic myeloid leukemia has improved significantly with the introduction of tyrosine kinase inhibitors (TKIs), and treatment guidelines based on numerous clinical trials are available for chronic phase disease. However for CML in the blast phase (CML-BP), prognosis remains poor and treatment options are much more limited. The spectrum of treatment strategies for children and adolescents with CML-BP has largely evolved empirically and includes treatment principles derived from adult CML-BP and pediatric acute leukemia. Given this heterogeneity of treatment approaches, we formed an international panel of pediatric CML experts to develop recommendations for consistent therapy in children and adolescents with this high-risk disease based on the current literature and national standards. Recommendations include detailed information on initial diagnosis and treatment monitoring, differentiation from Philadelphia-positive acute leukemia, subtype-specific selection of induction therapy, and combination with tyrosine kinase inhibitors. Given that allogeneic hematopoietic stem cell transplantation currently remains the primary curative intervention for CML-BP, we also provide recommendations for the timing of transplantation, donor and graft selection, selection of a conditioning regimen and prophylaxis for graft-versus-host disease, post-transplant TKI therapy, and management of molecular relapse. Management according to the treatment recommendations presented here is intended to provide the basis for the design of future prospective clinical trials to improve outcomes for this challenging disease.".
• 01GSYW7H40H6Y9Q6WYB02AJSC5 abstract "In the last years, several research works have been proposed for the Knowledge Graph Completion task. However, like most Machine Learning models, most Knowledge Graph Completion models are opaque and lack interpretability. In order to achieve transparency, several interpretable and explainable models have been proposed. The Deterministic Local Interpretable Model-Agnostic Explanations (DLIME) was proposed to solve the lack of stability of the Local Interpretable Model-Agnostic Explanations (LIME), one of the most popular surrogate models. However, using DLIME to explain Machine Learning models in graphs becomes an issue due to its experiments being published only with tabular data. Therefore, this work aims to propose an interpretable method for graphs as an extension of DLIME named DLIME-Graphs. As a triple representation, DLIME-Graphs uses triple embeddings computed by SBERT which in turn, are reduced by the UMAP technique. Instead of using Hierarchical Clustering as DLIME, DLIME-Graphs uses HDB-SCAN to get clusters. To explain a test triple, DLIME-Graphs proposes to train two interpretable models: logistic regression and decision tree plus getting the most similar triples by a k-NN algorithm. The demonstration through a study case showed that DLIME-Graphs is able to give explanations for 100% of the triples in the test dataset through the former models offering transparency and interpretability.".
• 01GSYXEV4DWKFD263MBG8DR755 abstract "This study proposes that in order to increase compliance with government directives during health crises, we should consider the message as well as the messenger. Prior research shows that highly instructional crisis communication increases compliance during acute crisis situations. Crisis communication literature provides no clear answers on how to address prolonged crises like the COVID-19 pandemic, however. We examined the impact of crisis communication on compliance with health directives in March - April 2021, during the third wave of the pandemic in Belgium. Two field experiments were conducted at two different points in time. Participants received an informational message from Belgian authorities, that was either highly instructional or not. Next, they watched a set of messages from traditional and social media, depicting Belgian politicians setting a good or a bad example in terms of adhering to public health directives. Instructional messages only proved to be effective in the first study. Leading-by-example had a stronger impact, especially in study two. During the third wave of COVID-19, Belgian people seemed more inclined to follow public health directives when politicians set the right example, due to an increase in trust in the government. Bad examples, however, decreased compliance.".
• 01GSYXXET6JT7ENX3HZX2RK5M9 abstract "An intervention, SelfMED, was introduced to facilitate patient self-management of medication during hospitalization. This study aimed to evaluate the feasibility and safety of the SelfMED intervention. All patients in a cardiology ward in a Belgian regional hospital were assessed for suitability for inclusion, applying an evidence-based stepped assessment tool. Patients eligible for medication self-management and willing to participate were included in the study (i.e., consecutive sampling). Patients who self-managed their medication were closely monitored by nurses. The feasibility of medication self-management was evaluated by implementation and completion rates and the opinions of cardiologists. Safety was evaluated by medication administration errors and errors in patients' registration of intake. Of 159 patients assessed for eligibility to self-manage medication in-hospital, 61 were included. A total of 367 medicines were self-managed. Pill counts showed 3 administration errors (0.8%), and on 6 occasions (1.7%) the patient's registration of the intake was incorrect. SelfMED was deemed feasible within the hospital ward. In cardiologists' opinions, SelfMED requires substantial time investment. In summary, SelfMED facilitated patient medication self-management in-hospital. As an essential step in the preparation for a full trial, this study showed it is feasible and safe to implement the intervention and identified some possibilities for refinement.".
• 01GSYXXETDQMCFCB8X3H6388HX abstract "Background Allergic rhinitis (AR) management requires a coordinated effort from healthcare providers and patients. Pharmacists are key members of these integrated care pathways resolving medication-related problems, optimizing regimens, improving adherence and recommending therapies while establishing liaisons between patients and physicians. Methods Allergic Rhinitis and its Impact on Asthma (ARIA) first published a reference document on the pharmacist's role in allergic rhinitis management in 2004. Several guidelines were developed over the past 20 years improving the care of allergic rhinitis patients through an evidence-based, integrated care approach. Results This ARIA/EAACI/FIP Position Paper is based on the latest ARIA in the Pharmacy guidelines and provides: (a) a structured approach to pharmacists identifying people with AR and/or allergic conjunctivitis as well as those at risk of poor disease control; (b) an evidence-based clinical decision support tool for optimising the management of allergic rhinitis in the community pharmacy; and (c) a framework of referral to the physician. Conclusion This document is not intended to be a mandatory standard of care but is provided as a basis for pharmacists and their staff to develop relevant local standards of care for their patients, within their local practice environment. Pharmacy care varies between countries, and the guide should be adapted to the local situation.".
• 01GSYY0RHYMD48Z3EE062JDYYN abstract "Pharmacometric modelling plays a key role in both the design and analysis of regulatory trials in paediatric drug development. Studies in adults provide a rich source of data to inform the paediatric investigation plans, including knowledge on drug pharmacokinetics (PK), safety and efficacy. In children, drug disposition differs widely from birth to adolescence but extrapolating adult to paediatric PK, safety and efficacy either with pharmacometric or physiologically based approaches can help design or in some cases reduce the need for clinical studies. Aspects to consider when extrapolating PK include the maturation of drug metabolizing enzyme expression, glomerular filtration, drug excretory systems, and the expression and activity of specific transporters in conjunction with other drug properties such as fraction unbound. Knowledge of these can be used to develop extrapolation tools such as allometric scaling plus maturation functions or physiologically based PK. PK/pharmacodynamic approaches and well-designed clinical trials in children are of key importance in paediatric drug development. In this white paper, state-of-the-art of current methods used for paediatric extrapolation will be discussed. This paper is part of a conect4children implementation of innovative methodologies including pharmacometric and physiologically based PK modelling in clinical trial design/paediatric drug development through dissemination of expertise and expert advice. The suggestions arising from this white paper should define a minimum set of standards in paediatric modelling and contribute to the regulatory science.".
• 01GSYYDQ81YN21HM969E1QNZPE abstract "The black perovskite phase of CsPbI3 is promising for optoelectronic applications; however, it is unstable under ambient conditions, transforming within minutes into an optically inactive yellow phase, a fact that has so far prevented its widespread adoption. Here we use coarse photolithography to embed a PbI2-based interfacial microstructure into otherwise-unstable CsPbI3 perovskite thin films and devices. Films fitted with a tessellating microgrid are rendered resistant to moisture-triggered decay and exhibit enhanced longterm stability of the black phase (beyond 2.5 years in a dry environment), due to increasing the phase transition energy barrier and limiting the spread of potential yellow phase formation to structurally isolated domains of the grid. This stabilizing effect is readily achieved at the device level, where unencapsulated CsPbI3 perovskite photodetectors display ambient-stable operation. These findings provide insights into the nature of phase destabilization in emerging CsPbI3 perovskite devices and demonstrate an effective stabilization procedure which is entirely orthogonal to existing approaches.".
• 01GSYZKG5FPV969T4V3425DTAZ abstract "The building design is a crucial factor that can be actively adjusted and optimized to prevent human and property threats in emergency scenarios. Previous research suggests that specific building layouts may significantly influence human behaviour during evacuation. However, detailed empirical data about human behaviour in various types of buildings with different layouts are still missing and only marginal recommendations from this field are reflected in actual construction practice. In this study, desktop VR technologies were employed to study human decision-making in problematic T-intersections in the context of an emergency evacuation. More specifically, we studied fundamental attributes of buildings such as the width and length of the corridors and the presence of stairs to explore how they influence the choice of the evacuation route. The space-syntax isovist method was used to describe spatial parameters of corridors, which makes the results applicable to all buildings. Behavioural data from 208 respondents were analysed using multilevel regression models. Our results support previous claims concerning the importance of specific spatial layouts of evacuation corridors because respondents systematically chose wider and shorter corridors with visible staircases as the preferred evacuation route. The present findings further promote the ongoing discussion on the design of marked evacuation routes and building design that takes human factors into consideration.".
• 01GSZ0VFX6SJJR2PBA4RSHYQXJ abstract "In 1704, Mary Astell, known by many scholars as the "first English feminist," published Moderation Truly Stated, her contribution to the national debate over "occasional conformity." This was the practice of periodic participation in the sacraments of the Church of England-above all, taking communion-in order to become eligible for public office. This practice was defended as an exercise of the virtue of "moderation," viewed as the opposite of zeal and associated with politeness and reasonableness. In this article I recover Astell's critique on this new notion of moderation, as well as her own alternative conception of the virtue of moderation as scripture moderation, which she envisioned as zeal and indifference towards the right ends. My aim is threefold. First, to explore the dangers of conceiving of moderation as an "antidote to zeal," which Astell argued would be detrimental to truth, salvation, and moral progress. Second, to demonstrate that her own conception of moderation as zeal and indifference towards the right ends was a radical subversion of the discourse on moderation at the time. Third, to shed light on the role of the Occasional Conformity debate in the transformation of moderation from a Christian virtue of temperance and control into a "modern" virtue construed as politeness and opposed to zeal, which was to become dominant in eighteenth-century England.".
• 01GSZ2XFKT82FMAHYYV4FVBZGZ abstract "Our bodies turn over billions of cells daily via apoptosis and are in turn cleared by phagocytes via the process of "efferocytosis."Defects in efferocytosis are now linked to various inflammatory diseases. Here, we de-signed a strategy to boost efferocytosis, denoted "chimeric receptor for efferocytosis"(CHEF). We fused a specific signaling domain within the cytoplasmic adapter protein ELMO1 to the extracellular phosphatidyl-serine recognition domains of the efferocytic receptors BAI1 or TIM4, generating BELMO and TELMO, respectively. CHEF-expressing phagocytes display a striking increase in efferocytosis. In mouse models of inflammation, BELMO expression attenuates colitis, hepatotoxicity, and nephrotoxicity. In mechanistic studies, BELMO increases ER-resident enzymes and chaperones to overcome protein-folding-associated toxicity, which was further validated in a model of ER-stress-induced renal ischemia-reperfusion injury. Finally, TELMO introduction after onset of kidney injury significantly reduced fibrosis. Collectively, these data advance a concept of chimeric efferocytic receptors to boost efferocytosis and dampen inflammation.".
• 01GSZ8RZEX6FM1M91EHR6MYZW7 abstract "Microglia and border-associated macrophages (BAMs) are brain-resident self-renewing cells. Here, we examined the fate of microglia, BAMs, and recruited macrophages upon neuroinflammation and through resolution. Upon infection, Trypanosoma brucei parasites invaded the brain via its border regions, triggering brain barrier disruption and monocyte infiltration. Fate mapping combined with single-cell sequencing revealed microglia accumulation around the ventricles and expansion of epiplexus cells. Depletion experiments using genetic targeting revealed that resident macrophages promoted initial parasite defense and subsequently facilitated monocyte infiltration across brain barriers. These recruited monocyte-derived macrophages outnumbered resident macrophages and exhibited more transcriptional plasticity, adopting antimicrobial gene expression profiles. Recruited macrophages were rapidly removed upon disease resolution, leaving no engrafted monocyte-derived cells in the parenchyma, while resident macrophages progressively reverted toward a homeostatic state. Long-term transcriptional alterations were limited for microglia but more pronounced in BAMs. Thus, brain-resident and recruited macrophages exhibit diverging responses and dynamics during infection and resolution.".
• 01GSZ8VJHT188WF9X7HFWECK3T abstract "To achieve optimal vitrification, tissue structure and fragment size represent a challenge for obtaining sufficient cooling velocity. Theoretically, thin ovarian tissue fragments lead to higher surface contact, hence higher solute penetration. Another critical factor is the concentration of cryoprotectants (CPA): CPA toxicity may occur with high concentrations, and as such, this may induce local apoptosis. Therefore two experiments were conducted: In experiment I, we compared the effect of sucrose supplementation in vitrification solution along with ovarian fragments of different sizes on post-warming tissue viability and follicle architecture. Fragments of two different sizes, with a thickness and radius of 1.5 x 0.75 mm and 3 x 1.5 mm respectively were vitrified in vitrification solution without sucrose and with 0.5 M sucrose supplementation. Post-warming, fragments of ovarian tissue (fresh and vitrified) were evaluated for viability (Calcein AM/Propidium Iodide) and for morphology (hematoxylin-eosin). In experiment II, we aimed to reduce cryoprotectant toxicity by using lower CPA concentrations in combination with an optimized carrier medium (HypThermosol (R); HTS). Ovarian tissue fragments were randomly allocated to five groups (A: fresh controls; B: vitrified in GLOBAL (R) TOTAL (R) LP w/HEPES with 15% ethylene glycol (EG) and 15% DMSO; C: vitrified in HTS with 5% EG and 5% DMSO; D: vitrified in HTS with 10% EG and 10% DMSO; E: vitrified in HTS with 15% EG and 15% DMSO). Fragments (fresh and vitrified) were evaluated for morphology (hematoxylin-eosin) and for apoptosis through the activity of caspase-3. Results showed that follicular morphology was affected by the size of the fragment; smaller sized fragments contained a greater proportion of intact follicles (53.8 +/- 2.0%) compared to the larger fragments (40.3 +/- 2.0%). Our results demonstrated that 1.5 x 0.75 mm sized pieces vitrified in a vitrification solution supplemented with 0.5 M sucrose had more intact follicles (54.8 +/- 1.3%; P = 0.0002) after vitrification. In addition, HTS presented no additional protective effect as a base medium, neither for follicular morphology nor apoptotic rate. (c) 2022 Elsevier Inc. All rights reserved.".
• 01GSZ8XNFJQR50E3BP84YVGMA1 abstract "Organs-on-a-chip (OoC) are cell culture platforms that replicate key functional units of tissues in vitro. Barrier integrity and permeability evaluation are of utmost importance when studying barrier-forming tissues. Impedance spectroscopy is a powerful tool and is widely used to monitor barrier permeability and integrity in real-time. However, data comparison across devices is misleading due to the generation of a non-homogenous field across the tissue barrier, making impedance data normalization very challenging. In this work, we address this issue by integrating PEDOT:PSS electrodes for barrier function monitoring with impedance spectroscopy. The semitransparent PEDOT:PSS electrodes cover the entire cell culture membrane providing a homogenous electric field across the entire membrane making the cell culture area equally accountable to the measured impedance. To the best of our knowledge, PEDOT:PSS has never been used solely to monitor the impedance of cellular barriers while enabling optical inspection in the OoC. The performance of the device is demonstrated by lining the device with intestinal cells where we monitored barrier formation under flow conditions, as well as barrier disruption and recovery under exposure to a permeability enhancer. The barrier tightness and integrity, and the intercellular cleft have been evaluated by analyzing the full impedance spectrum. Furthermore, the device is autoclavable paving the way toward more sustainable OoC options.".
• 01GSZ9SDFZW4W7JSME1S5ZAN2S abstract "Cuba, and more specifically Havana, is known throughout the world for its rich architectural heritage. The 214 ha centre of Havana, consisting of primarily housing, is inscribed as UNESCO world heritage. Nevertheless, is Cuba suffering from a major housing crisis. 70% of the built environment requires major repairs and 7% of the housing context has been declared uninhabitable. In order to tackle the degradation problem, while simultaneously protecting the historical heritage, there is an urgent need for tools and practical guidelines. These should provide insight into the approach to conserving Havana’s heritage and the risks that could entail. The reasons for this deterioration and damage include different moisture-related problems due to the hot and humid climate of Cuba: mould growth, salt efflorescence and the rotting of wooden beam heads embedded in the masonry of the façade. Heat Air Moisture (HAM) models have been found a valuable tool to assess the risk on deterioration and premature failure of building components due to moisture-related problems. This paper demonstrates the process from hygrothermal simulation to practical guideline. The study focuses on the potential of the practical applicability of simulation output from Delphin 6, to produce practical schemes using classification trees. These results will improve decision-making for building practitioners and clarify which parameters have the biggest impact on the risks of deterioration, thus providing insights into the conservation of heritage buildings of Havana, Cuba.".
• 01GSZAE4CN0AQ56B9V5BP0VH3H abstract "Various studies indicate that climate change can have a significant impact on the damage risks in solid masonry facades. Heat Air Moisture (HAM) models have been found a valuable tool to assess the risk on deterioration and premature failure of building components due to freeze-thaw action, mould growth, wood decay etc. However, these simulations require specific software and expertise, which hampers to massive uptake by building practitioners. Next to that, the large number of material properties and boundary conditions and the non-linear nature of the output render it difficult to derive simple guidelines for the building industry. This paper builds on previous research that examined the impact of climate change on the hygrothermal behaviour of masonry walls with a factorial study on the freeze-thaw risk, mould growth and wood decay, for Brussels, Belgium. Different parameters such as masonry thickness, insulation thickness, orientation, greenhouse gas emission scenarios, brick type and rain exposure were varied, resulting in 8192 simulations in Delphin 6.1. This paper focuses on the potential and the development of a clear decision framework for the practical application of the above-mentioned research using classification trees. These results will improve decision-making for building practitioners and will further clarify which parameters have the most significant impact on the deterioration risks. Next to that, the results provide insights into which types of buildings are most at risk due to climate change, and when the application of interior insulation should be avoided.".
• 01GSZAGHCG1NCFA3XT30EBYJZ0 abstract "The choroid plexus (CP), a highly vascularized structure protruding into the ventricles of the brain, is one of the most understudied tissues in neuroscience. As it is becoming increasingly clear that this tiny structure plays a crucial role in health and disease of the central nervous system (CNS), it is of utmost importance to properly dissect the CP out of the brain ventricles in a way that allows downstream processing, ranging from functional to structural analysis. Here, isolation of the lateral and fourth brain ventricle mouse CP without the need for specialized tools or equipment is described. This isolation technique preserves the viability, function, and structure of cells within the CP. On account of its high vascularization, the CP can be visualized floating inside the ventricular cavities of the brain using a binocular microscope. However, transcardial perfusion required for downstream analysis can complicate the identification of the CP tissue. Depending on the further processing steps (e.g., RNA and protein analysis), this can be solved by visualizing the CP via transcardial perfusion with bromophenol blue. After isolation, the CP can be processed using several techniques, including RNA, protein, or single cell analysis, to gain further understanding on the function of this special brain structure. Here, scanning electron microscopy (SEM) on whole mount CP is used to get an overall view of the structure.".
• 01GSZBHN2T747W7APW0M7WK7CB abstract "Broiler chickens are among the main livestock sectors worldwide. With individual treatments being inapplicable, contrary to many other animal species, the need for antimicrobial use (AMU) is relatively high. AMU in animals is known to drive the emergence and spread of antimicrobial resistance (AMR). High farm biosecurity is a cornerstone for animal health and welfare, as well as food safety, as it protects animals from the introduction and spread of pathogens and therefore the need for AMU. The goal of this study was to identify the main biosecurity practices associated with AMU in broiler farms and to develop a statistical model that produces customised recommendations as to which biosecurity measures could be implemented on a farm to reduce its AMU, including a cost-effectiveness analysis of the recommended measures. AMU and biosecurity data were obtained cross-sectionally in 2014 from 181 broiler farms across nine European countries (Belgium, Bulgaria, Denmark, France, Germany, Italy, the Netherlands, Poland and Spain). Using mixed-effects random forest analysis (Mix-RF), recursive feature elimination was implemented to determine the biosecurity measures that best predicted AMU at the farm level. Subsequently, an algorithm was developed to generate AMU reduction scenarios based on the implementation of these measures. In the final Mix-RF model, 21 factors were present: 10 about internal biosecurity, 8 about external biosecurity and 3 about farm size and productivity, with the latter showing the largest (Gini) importance. Other AMU predictors, in order of importance, were the number of depopulation steps, compliance with a vaccination protocol for non-officially controlled diseases, and requiring visitors to check in before entering the farm. K-means clustering on the proximity matrix of the final Mix-RF model revealed that several measures interacted with each other, indicating that high AMU levels can arise for various reasons depending on the situation. The algorithm utilised the AMU predictive power of biosecurity measures while accounting also for their interactions, representing a first step toward aiding the decision-making process of veterinarians and farmers who are in need of implementing on-farm biosecurity measures to reduce their AMU.".
• 01GSZC9N83V2P2BTYE8Q7M7Q73 abstract "Objectives:As children with sensorineural hearing loss have an increased risk for vestibular impairment, the Vestibular Infant Screening-Flanders project implemented a vestibular screening by means of cervical vestibular evoked myogenic potentials (cVEMP) at the age of 6 months for each child with hearing loss in Flanders (Belgium). Given that vestibular deficits can affect the child's development, this vestibular screening should allow early detection and intervention. However, less is currently known about which screening tool would be the most ideal and how vestibular impairment can evolve. Therefore, this study aimed to determine the most appropriate tool to screen for vestibular deficits, to assess the necessity of vestibular follow-up, and to set clinical guidelines for vestibular screening in children with hearing loss. Design:In total, 71 children with congenital or early-onset sensorineural hearing loss were enrolled (mean age at first appointment = 6.7 months). Follow-up was provided at 6 months, 1, 2, and 3 years of age. Below three years of age, the video Head Impulse Test (vHIT) of the horizontal semicircular canals (SCC), the cVEMP, and the rotatory test at 0.16, 0.04, and 0.01 Hz were applied. At 3 years of age, the vHIT of the vertical SCC and ocular vestibular evoked myogenic potentials (oVEMP) were added. To evaluate early motor development, the Alberta Infant Motor Scale (AIMS) results at 6 months and 1-year old were included. Results:At 6 months of age, the highest success rate was obtained with the cVEMP (90.0%) compared to the vHIT (70.0%) and the rotatory test (34.3-72.9%). Overall, vestibular deficits were found in 20.0% of the children, consisting of 13.9% with both SCC and otolith deficits (bilateral: 9.3%, unilateral: 4.6%), and 6.1% with unilateral isolated SCC (4.6%) or otolith (1.5%) deficits. Thus, vestibular deficits would not have been detected in 4.6% of the children by only using the cVEMP, whereas 1.5% would have been missed when only using the vHIT. Although vestibular deficits were more frequently found in severe to profound hearing loss (28.6%), characteristics of vestibular function were highly dependent on the underlying etiology. The AIMS results showed significantly weaker early motor development in children with bilateral vestibular deficits (p = 0.001), but could not differentiate children with bilateral normal vestibular function from those with unilateral vestibular deficits (p > 0.05). Progressive or delayed-onset vestibular dysfunction was only found in a few cases (age range: 12-36 months), in which the hearing loss was mainly caused by congenital cytomegalovirus (cCMV). Conclusions:The cVEMP is the most feasible screening tool to assess vestibular function in 6-months-old children with hearing loss. Although the majority of children with vestibular deficits are detected with the cVEMP, the vHIT seems even more sensitive as isolated SCC deficits are associated with specific etiologies of hearing loss. As a result, the cVEMP is an appropriate vestibular screening tool, which is advised at least in severe to profound hearing loss, but certain etiologies require the addition of the vHIT (i.e., cCMV, meningitis, cochleovestibular anomalies with or without syndromic hearing loss).".
• 01GSZE7A4BKQNQ216Z7QBNNQ70 abstract "Immune checkpoint blockade (ICB) has heralded a new era in cancer therapy. Research into the mechanisms underlying response to ICB has predominantly focused on T cells; however, effective immune responses require tightly regulated crosstalk between innate and adaptive immune cells. Here, we combine unbiased analysis of blood and tumors from metastatic breast cancer patients treated with ICB with mechanistic studies in mouse models of breast cancer. We observe an increase in systemic and intratumoral eosinophils in patients and mice responding to ICB treatment. Mechanistically, ICB increased IL-5 production by CD4+ T cells, stimulating elevated eosinophil production from the bone marrow, leading to systemic eosinophil expansion. Additional induction of IL-33 by ICB-cisplatin combination or recombinant IL-33 promotes intratumoral eosinophil infiltration and eosinophil-dependent CD8+ T cell activation to enhance ICB response. This work demonstrates the critical role of eosinophils in ICB response and provides proof-of-principle for eosinophil engagement to enhance ICB efficacy.".
• 01GSZMR10HNBCQ3V9N4WMB9YSM abstract "Sterke bevolkingsaangroei, klimaatsverandering, en verandering in landgebruik vooral door uitbreiding van de landbouw, zetten de duurzaamheid van de beperkte watervoorraden van ontwikkelingslanden zoals Ethiopië onder druk. Het bekken van Lake Tana wordt beschouwd als een van de landbouwkundige groei-corridors van het land. In recente jaren is de waterwinning exponentieel toegenomen in het bekken. Studies over de watervoorraden, vooral over grondwater, zijn beperkt en lijden onder het gebrek aan gegevens. Watervoorraden worden verwacht sporadisch te zijn verdeeld als gevolg van de complexe geologie en topografie. Dus vormt het een uitdaging voor het waterbeheer om de spatiale verdeling van de watervoorraden in kaart te brengen. In dit onderzoek werden de spatiaal verdeelde grondwatervoeding en andere waterbalanscomponenten gesimuleerd met een fysisch gebaseerd waterbalansmodel. De betrouwbaarheid van de gesimuleerde voeding wordt gevalideerd met puntwaarden gebaseerd op de WaterTafel Fluctuatie en Chloride MassaBalans methoden. Spatiaal sterk variabele grondwatervoeding wordt bekomen. Pompproeven en slagproeven worden uitgevoerd, en de hydraulische doorlatendheid en transmissiviteit van de ondiepe aquifers worden afgeleid. Belangrijke inzichten in het functioneren van het grondwatersysteem werden verkregen uit de interpretatie van de grondwater hydrogrammen. Tenslotte werd een steady-state grondwaterstromingsmodel gebruikt om de verschillende grondwaterbalanscomponenten te simuleren. Bovendien werden de oppervlaktewater-grondwater interacties (rivier en meer met het grondwater) ingeschat met het MODFLOW model.".
• 01GSZQFHY12MMST1Y4R336JWQZ abstract "Terrestrial gastropods are hosts of a wide variety of metazoan parasites and can respond to parasite exposure in various ways. One of these defence mechanisms, the ability to trap parasites in the host shell, was previously thought to apply only against nematodes. During a field survey along an urbanisation gradient, we found that the shell of Cornu aspersum and Cepaea nemoralis can contain encapsulated trematode cercariae, with prevalences of 7% and 1%, respectively over the entire sample, and up to 47% at the local population level. To our knowledge, this is the first case study unambiguously showing that land snails can trap non-nematode parasites in their shell at non-negligible prevalences. Shell-encapsulation could be a more general defence mechanism than previously described, and more studies are needed to understand its importance and variability.".
• 01GSZR7RX124B9WNG69XN3VCVG abstract "Dispersal and its evolution play a key role for population persistence in fragmented landscapes where habitat loss and fragmentation increase the cost of between-habitat movements. In such contexts, it is important to know how variation in dispersal and other traits is structured, and whether responses to landscape fragmentation are aligned with underlying dispersal-trait correlations, or dispersal syndromes. We, therefore, studied trait variation in Erigone longipalpis, a European spider species specialist of (often patchy) salt marshes. We collected spiders in two salt-marsh landscapes differing in habitat availability. We then reared lab-born spiders for two generations in controlled conditions, and measured dispersal and its association with various key traits. Erigone longipalpis population densities were lower in the more fragmented landscape. Despite this, we found no evidence of differences in dispersal, or any other trait we studied, between the two landscapes. While a dispersal syndrome was present at the among-individual level (dispersers were more fecund and faster growing, among others), there was no indication it was genetically driven: among-family differences in dispersal were not correlated with differences in other traits. Instead, we showed that the observed phenotypic covariations were mostly due to within-family correlations. We hypothesize that the dispersal syndrome is the result of asymmetric food access among siblings, leading to variation in development rates and carrying over to adult traits. Our results show we need to better understand the sources of dispersal variation and syndromes, especially when dispersal may evolve rapidly in response to environmental change.".
• 01GSZRM7RKN078AHH2RXJN9SVW abstract "Host behaviour can be influenced by parasitic risk and infection through a variety of direct and indirect mechanisms. We can expect individuals expressing different phenotypes to also differ in the ways their behaviour is altered by parasites. We used the land snail Cepaea nemoralis, a species with various shell colour morphs differing in behaviour and physiology, as a model to study the link between parasite response and individual behaviour variation. We analysed metazoan parasite abundance and its relation to behaviour (movement and food intake) in snails from three shell morphs (from light unbanded to darker five-banded) and from two neighbouring populations from contrasting environments. Snails were parasitized by mites, trematodes, and nematodes, from rarest to most frequent. We confirm that terrestrial gastropods can defend against infection by trapping parasitic nematodes in their shell. We show that nematodes encapsulated in shells can uncover past infections even when a snail population is currently nematode-free, possibly due to parasite seasonality. We present new observations suggesting that not only nematodes but also mites might be encapsulated in shells. Infection levels varied between morphs, with darker snails harbouring fewer nematodes. Behaviour (movement and food intake) was linked to the nematode, but not trematode infection. Individuals with higher nematode load ate less, irrespective of morph and population. The most-infected morph (unbanded snails) showed reduced activity in the nematode-infected population compared to the one that was nematode-free at sampling time. As a result, parasites may cancel previously described behavioural differences between morphs. We discuss the possible mechanisms explaining morph-dependent responses to parasites, and how parasite risk may be an unseen force shaping C. nemoralis morph variation in space and time. We conclude with the possible ecological consequences of the link, mediated by shell colour, and between thermal and immune responses.".
• 01GSZV96Q65B3GQJRZ4P3A8GAJ abstract "This contribution examines the process of banishment as part of the political tools available to an aldermen bench to control the city. Banishment was a punishment that closely corresponded to the urban ideology where undesirable and even harmful elements were literally removed from the community. It was when a dissident administration came to power in 1477 that banishments became more frequent. These insurgents used punishment to stigmatise the previous regime as corrupt and morally reprehensible and to discipline their own populace. Banishments were used to eliminate subversive elements from the city, but also to punish bad behaviour. The most striking aspect here is the moralization. The Ghent aldermen who were in power between 1477 and 1485 banished many more people for immoral behaviour such as adultery than their predecessors and their descendants. The article argues that the lack of legitimacy suffered by the insurgents encouraged this phenomenon. These insurgents tried to communicate a message of law and order. In doing so, they presented themselves as good governors.".
• 01GT17FEMYEC6ED83C80PSZC5J abstract "Simple Summary A significant percentage of Dutch partridge dogs suffers from epileptic seizures. Previous studies indicated that this is likely the result of genetic idiopathic epilepsy, but to date, little to no knowledge exists on the genetic cause. This study aimed to identify (a) causal variant(s) and/or risk loci associated with the disease. A risk locus for idiopathic epilepsy was described for the first time on chromosome 12. Furthermore, a variant in the CCDC85A gene was found to increase the risk of disease in homozygous variant dogs. Further research should be conducted to determine whether the chromosome 12 risk locus and CCDC85A variant can be used in breeding decisions. (1) Idiopathic epilepsy (IE) is thought to have a genetic cause in several dog breeds. However, only two causal variants have been identified to date, and few risk loci are known. No genetic studies have been conducted on IE in the Dutch partridge dog (DPD), and little has been reported on the epileptic phenotype in this breed. (2) Owner-filled questionnaires and diagnostic investigations were used to characterize IE in the DPD. A genome-wide association study (GWAS) involving 16 cases and 43 controls was performed, followed by sequencing of the coding sequence and splice site regions of a candidate gene within the associated region. Subsequent whole-exome sequencing (WES) of one family (including one IE-affected dog, both parents, and an IE-free sibling) was performed. (3) IE in the DPD has a broad range in terms of age at onset, frequency, and duration of epileptic seizures. Most dogs showed focal epileptic seizures evolving into generalized seizures. A new risk locus on chromosome 12 (BICF2G630119560; p(raw) = 4.4 x 10(-7); p(adj) = 0.043) was identified through GWAS. Sequencing of the GRIK2 candidate gene revealed no variants of interest. No WES variants were located within the associated GWAS region. However, a variant in CCDC85A (chromosome 10; XM_038680630.1: c.689C > T) was discovered, and dogs homozygous for the variant (T/T) had an increased risk of developing IE (OR: 6.0; 95% CI: 1.6-22.6). This variant was identified as likely pathogenic according to ACMG guidelines. (4) Further research is necessary before the risk locus or CCDC85A variant can be used for breeding decisions.".
• 01GT198ZVJWBVK1YXCVH2VFTKR abstract "In addition to Helicobacter pylori, many non-Helicobacter pylori Helicobacters (NHPH) are able to cause gastric disease in humans. Cats are a natural reservoir for many of these species. Accordingly, living in close and intimate contact with animals has been identified as a risk factor, and an important zoonotic significance has therefore been attributed to NHPH. To determine the prevalence and associated gastric histopathological changes of Helicobacter species, the gastric mucosa of 71 cats were evaluated. Only four presented normal histopathological mucosa with the absence of spiral-shaped organisms. Normal gastric mucosa and the presence of spiral-shaped bacteria were observed in 13 cats. The remaining animals presented histopathological changes representative of gastritis. Helicobacter species were detected in 53 cats (74.6%) by at least one detection method. None of the animals were positive for H. pylori or for H. ailurogastricus. Helicobacter heilmannii organisms were identified in 20 animals, predominantly in the body gastric region. Helicobacter salomonis was the second most prevalent species (57.1%), although it was mainly found in association with other NHPH. Helicobacter felis and H. bizzozeronii were less frequently detected. The great majority of the Helicobacter spp. PCR-positive animals presented normal features regarding fibrosis/mucosal atrophy, neutrophils, eosinophils, or other inflammatory cells and lymphofollicular hyperplasia. Given the controversy and the strong evidence of absence of significant histopathological alterations associated with the presence of Helicobacter spp. in cats, it is possible to hypothesize that these bacteria may be able to adapt to the feline gastric microenvironment or even to comprise part of the gastric microbiome of this animal species. Thus, prudency must be taken when prescribing an antibiotic therapy based solely on the presence of these bacteria in the feline stomach.".
• 01GT19F52RTJ6FF663VQ1DRW4H abstract "Carbohydrate antigen 19-9 (CA 19-9) is a biological marker used to diagnose and monitor the progression of various cancers. Elevated CA 19-9 has also been sporadically observed in Helicobacter pylori infected patients. Similar to H. pylori, animal-hosted non-H. pylori Helicobacter (NHPH) species can induce gastroduodenal lesions in humans. We report the first case of CA 19-9 elevation related to H. suis gastritis and its normalisation after eradication. A CA 19-9 screening prescribed as part of a regular check up by the general practitioner was found elevated in a 68-year-old man presenting chronic dyspeptic symptoms. Medical investigations were negative for presence of neoplasia or biliary obstruction. Upper gastrointestinal endoscopy confirmed the presence of chronic gastritis and H. suis was identified in gastric biopsies. The standard treatment for H. pylori successfully eradicated H. suis with normalisation of CA 19-9 levels. In addition to H. pylori, infection with NHPH species should be considered as an additional cause of elevated CA19-9. (Acta gastroenterol. belg., 2022, 85, 403-405).".
• 01GT19KKX679VEBHRMGBVB83HF abstract "Ferroptosis has gained interest due to it immunogenicity and the higher sensitivity of cancer cells to it. However, it was recently shown that ferroptosis in tumor-associated neutrophils leads to immunosuppression and negatively impacts therapy. Here, we discuss the potential implications of the two sides (friend versus foe) of ferroptosis in cancer immunotherapy.".
• 01GT19M5FWHBEZ3HHE4T2TW1F6 abstract "Mitigating global amphibian declines requires a thorough understanding of stressors that differentially impact amphibian populations. We compared body condition and occurrence of the chytrid fungus Batrachochytrium dendrobatidis (Bd) in syntopic frogs, belonging either to the small range, vulnerable species Pelophylax shqipericus or the widespread P. ridibundus. Bd was detected more frequently in P. shqipericus than in P. ridibundus (15.6% and 2.9%, p < 0.05, respectively). Infections were characterized by low infection intensity, absence of clinical signs and lack of any obvious impact on body condition. However, body condition in P. shqipericus was significantly lower than in P. ridibundus. Relatively high pathogen prevalence and poor body condition in the threatened P. shqipericus is worrying and urges the need for close monitoring, risk analysis and population assessment.".
• 01GT19P53X2JX2ZG7FECY05S01 abstract "The remoteness and isolation of South American tabletop mountain (tepui) summits may protect against infections that underpin global amphibian declines. Increases in recreational pressure in such unspoiled destinations, and in isolated ecosystems globally, pose a poorly understood risk of wildlife disease introduction, especially in supposedly immunologically naive communities. We here report the first observed chytrid infections in the Pantepui biogeographical region. Infections significantly correlate with proximity to the nearest basic tourist infrastructure in four endemic amphibians occurring on tepui summits and their slopes. Phylogenetic relationships and environmental context suggest a high risk of severe and irreversible population declines in unique, early branching amphibians. These findings advocate for an urgent control of recreational pressure in isolated, highly vulnerable ecosystems.".
• 01GT19STT9X85W92767KGQ190R abstract "Although, historically, Methicillin-Resistant Staphylococcus aureus (MRSA) was restricted to humans, since 2005 these strains emerged in livestock and wildlife. Therefore, a One Health approach was applied to analyze the diversity and characteristics of S. aureus strains isolated from the invasive species of mongoose (Urva auropunctata) in St. Kitts. Fecal samples collected from these animals (n = 81) were cultured on selective agar. The isolated S. aureus strains were identified using MALDI-TOF and further characterized by whole genome sequence analysis. The fecal microbiome study identified the presence of S. aureus in 5 animals. Both MSSA (n = 3) and MRSA (n = 2) strains were identified. The two MRSA isolated were nearly identical ST5 SCCmec IVa (2B) strains. The two MSSA isolated were a new ST7434, pertaining to clonal complex 30, and the other belonged to ST5, but unrelated to the MRSA ST5. The SCCmec IVa (2B) is, however, the main SCCmec in human MRSA of different STs identified in St Kitts, indicating potential horizontal transmission events. In conclusion, a new type of MSSA, ST7434, was found and MRSA ST5 t002 SCCmec IVa (2B) found its way into wildlife on a small Caribbean Island. Further One Health studies are necessary to determine the role of MRSA in wildlife.".
• 01GT19XE1AYZDWGTHJTJNCR4MK abstract "In recent years, there has been an increase in the number of outbreaks of avian influenza in indus-trial poultry flocks and backyard poultry in Europe. Also, the period of seasonal outbreaks seems to expand. In recent years, the virus has been circulating in the wild-bird population in Belgium, during summer time. Veterinarians are therefore urged to be watchful for this notifiable disease. In this review, an overview is given of the current epidemiological situation, the possible disease features, actions to be taken in case of suspicion of an outbreak and governmental control measures.".
• 01GT19ZNVJ5F6PXDCG3T8DAT8X abstract "Eimeria (E.) stiedae is a protozoan parasite causing hepatic coccidiosis in rabbits. It mostly infects younger animals and causes nonspecific signs like stunted growth, weakness, dehydration, diarrhea and anorexia. Macroscopically, the liver has a typical appearance. It is enlarged, showing firm yellow-white lesions on the surface. These lesions are enlarged bile ducts filled with bile and necrotic debris. Although all diagnostic tools for hepatic coccidiosis in live animals are currently impracticable or inconclusive, ultrasound might be useful for the diagnosis of hepatic coccidiosis. However, the appearance of liver changes associated with E. stiedae on ultrasonography has poorly been described in the literature. In this study, ex-vivo ultrasound of 24 livers was performed, i.e. the livers of two healthy rabbits and 22 livers of rabbits with suspected liver coccidiosis. Hyperechoic lesions of variable size and shape were found in all affected livers. In some of these livers, other signs of hepatic disease were detected: heterogenous liver parenchyma, appearance of hepatomegaly with round edges, gallbladder sludge and thickening of the gallbladder wall.".
• 01GT1A2C3GCWVZZFQMYVE8058Y abstract "Anorexia and ileus are common postoperative complications in rabbits and can be fatal if left untreated. Risk factors, like stress and pain, need to be minimized or avoided as much as possible. To get an insight into potential risk factors related to the anesthetic protocol for orchidectomy and ovario(hyster)ectomy (OV(H)X), a questionnaire was sent to veterinarians in Belgium and the Netherlands. Based on 209 answered questionnaires, a significant difference in the development of anorexia and ileus could be seen depending on the type of surgery (less in orchidectomy than in OV(H)X). Two other significant differences were noted: ovario(hyster)ectomized rabbits less frequently developed ileus when butorphanol was used, and more frequently developed anorexia when an antidote was used. This must however be interpreted carefully, since other study-related factors may have played a role. When considering whether or not buprenorphine - an opioid drug for which contradictory results have been reported in the literature - induces gastrointestinal stasis in rabbits, it is noteworthy that in this study, ileus was not reported to be a significant, more frequently noted side effect.".
• 01GT1A4HJSF0QD5GPHYZQZJ7K2 abstract "Simple Summary Antimicrobial resistance is of critical concern for both human and veterinary medicine worldwide. Many bacterial infections are currently very difficult to treat due to the presence of several mechanisms involved in bacterial resistance to marketed antimicrobials. Preserving the effectiveness of currently available antimicrobials and reducing the burden of infections caused by resistant bacteria is a priority for competent authorities operating in both sectors. It has been perceived that the livestock sector is a primary contributor to the spread of bacterial resistance in both humans and the environment. This review examined the recent scientific literature on this topic in order to explore the relationship between antimicrobials use in farm animals and the selection of bacterial resistance in this sector and its subsequent dissemination to humans. Recent data indicated that the global biomass-adjusted amount of antimicrobials consumed by farm animals was slightly higher than the amounts used in humans, while the reel contribution of farm animals in the spread of AMR to humans is probably very low compared to initial estimations. However, this review highlights the importance of the close collaboration between veterinary and human medicine, as part of the 'One Health' approach, to preserve the longevity of antimicrobials. Antimicrobial resistance (AMR) represents a global threat to both human and animal health and has received increasing attention over the years from different stakeholders. Certain AMR bacteria circulate between humans, animals, and the environment, while AMR genes can be found in all ecosystems. The aim of the present review was to provide an overview of antimicrobial use in food-producing animals and to document the current status of the role of farm animals in the spread of AMR to humans. The available body of scientific evidence supported the notion that restricted use of antimicrobials in farm animals was effective in reducing AMR in livestock and, in some cases, in humans. However, most recent studies have reported that livestock have little contribution to the acquisition of AMR bacteria and/or AMR genes by humans. Overall, strategies applied on farms that target the reduction of all antimicrobials are recommended, as these are apparently associated with notable reduction in AMR (avoiding co-resistance between antimicrobials). The interconnection between human and animal health as well as the environment requires the acceleration of the implementation of the 'One Health' approach to effectively fight AMR while preserving the effectiveness of antimicrobials.".
• 01GT1A7952T650DEY9GY9WD5ND abstract "Simple Summary Staphylococci are present in the microbiota of both humans and animal species, being recognized as the most important opportunistic pathogens. Antimicrobial resistance (AMR) has become a global public health issue presenting a significant risk because it severely limits treatment options. Methicillin resistance in staphylococci (MRS) poses a specific problem as it may cause serious human and animal infections, eventually resulting in death. The increasing observation of MRS in different animal species has raised the concern of their impact on animal health and the potential of zoonotic transmission. The availability of comprehensive data on the ecology and distribution of MRS in animals and food products worldwide is necessary to understand their relevance in the "One Health" domain. However, there is a gap in information in terms of MRS and the Arab countries. Therefore, our study aimed to provide an overview of the situation of MRS in these countries by reviewing the available data on livestock and animal products and making recommendations for the future. The prevalence of methicillin resistance in staphylococci has been increasing globally and is currently one of the major public health concerns. In particular, treating infections caused by staphylococci with acquired antimicrobial resistance is problematic, as their treatment is more difficult. The resistance is found both in human and animal staphylococcal strains. Methicillin-resistant staphylococci (MRS) have also been increasingly reported in wildlife. In Arab countries, MRS has been detected in food producing animals and food products; however, the risk this poses is somewhat unclear, and still a significant lack of information on the trend and distribution of these pathogens in these countries, which have a specific ecosystem (desert) and traditions (Muslim culture). In this manuscript, we aim to provide an overview of the prevalence and the major MRS clonal lineages circulating in these specific countries and compare to them other situations with different ecosystems and cultures.".
• 01GT1AA0165Z50Q4AZJPJQREET abstract "Annually, approximately 23,000 cases of food poisoning by Staphylococcus aureus enterotoxins are reported worldwide. The aim of this study was to determine the occurrence and characterize S. aureus on beef and beef products in South Africa. Organ meats (n = 169), raw processed meat (n = 110), raw intact (n = 53), and ready-to-eat meats (n = 68) were obtained from 25 retail outlets. S. aureus was isolated and enumerated according to the ISO 6888-1 method. Identification of the strains was performed by MALDI-TOF MS. The antimicrobial resistance was determined using the disc diffusion test. The presence of methicillin-resistance genes and the staphylococcal enterotoxin genes was determined by PCR. Prevalence was low (13/400; CI 1.7-5) and all but one positive sample were from organ meats. Eight isolates were resistant to at least one antibiotic. Two isolates carried the mecC gene. All the isolates tested positive for seg, seh, sei, and sep, whilst 53.8% were positive for sea. None of the isolates was positive for ser, sej, seb, sec, or sed. The prevalence of S. aureus was low, with organ meats being the most contaminated. The presence of mecC-positive MRSA and of enterotoxins warrants further investigation and risk assessment.".
• 01GT1ABVSKWDHE7Y3XE0NRHJKC abstract "Salmonellosis and antimicrobial resistance caused by non-typhoidal Salmonella are public health concerns. This study aimed at determining prevalence, serovars, virulence factors and antimicrobial resistance of Salmonella from beef products. Four-hundred beef samples from 25 retail outlets in KwaZulu-Natal, South Africa were analyzed for Salmonella using standard methods, confirmation with matrix-assisted laser desorption ionization-time of flight and serotyping according to the White-Kauffmann-Le Minor scheme. The Kirby Bauer disk diffusion method was used to determine antimicrobial resistance against Cefotaxime, Kanamycin, Ampicillin, Amoxicillin, Trimethoprim Sulfamethoxazole, Ciprofloxacin, Chloramphenicol, Gentamicin Cefoxitin and Tetracycline. A polymerase chain reaction was performed to detect invA, agfA, lpfA, hilA, sivH, sefA, sopE, and spvC virulence genes. Salmonella was observed in 1.25% (5/400) of the samples. Four serovars (Enteritidis, Hadar, Heidelberg, Stanley) were identified. Almost all Salmonella were susceptible to all antimicrobials except S. Enteritidis isolate that was resistant to Tetracycline, Ampicillin and Amoxicillin. All Salmonella isolates carried at least two virulence factors. The findings indicate low Salmonella prevalence in meat from selected KZN retail beef; however, routine surveillance to monitor risk associated with virulence factors is required to mitigate potential outbreaks. The resistant S. Enteritidis highlights a need to routinely monitor antimicrobial resistance in order to enhance human health.".
• 01GT1AE9A1G63MVY8HXW07AR7J abstract "The aim of this study was metagenomics analyses of acquired antibiotic-resistance genes (ARGs) in the intestinal microbiome of two important food-animal species in Hungary from a One Health perspective. Intestinal content samples were collected from 12 domestic pigs (Sus scrofa) and from a common carp (Cyprinus carpio). Shotgun metagenomic sequencing of DNA purified from the intestinal samples was performed on the Illumina platform. The ResFinder database was applied for detecting acquired ARGs in the assembled metagenomic contigs. Altogether, 59 acquired ARG types were identified, 51 genes from domestic pig and 12 genes from the carp intestinal microbiome. ARG types belonged to the antibiotic classes aminoglycosides (27.1%), tetracyclines (25.4%), beta-lactams (16.9%), and others. Of the identified ARGs, tet(E), a bla(OXA)-(48-like) beta-lactamase gene, as well as cphA4, ampS, aadA2, qnrS2, and sul1, were identified only in carp but not in swine samples. Several of the detected acquired ARGs have not yet been described from food animals in Hungary. The tet(Q), tet(W), tet(O), and mef(A) genes detected in the intestinal microbiome of domestic pigs had also been identified from free-living wild boars in Hungary, suggesting a possible relationship between the occurrence of acquired ARGs in domestic and wild animal populations.".
• 01GT1ARCCCH9HMZ4FBP6NZWC75 abstract "The microbial world represents a phenomenal diversity of microorganisms from different kingdoms of life, which occupy an impressive set of ecological niches. Most, if not all, microorganisms once colonize a surface develop architecturally complex surface-adhered communities, which we refer to as biofilms. They are embedded in polymeric structural scaffolds and serve as a dynamic milieu for intercellular communication through physical and chemical signalling. Deciphering microbial ecology of biofilms in various natural or engineered settings has revealed coexistence of microorganisms from all domains of life, including Bacteria, Archaea, and Eukarya. The coexistence of these dynamic microbes is not arbitrary, as a highly coordinated architectural setup and physiological complexity show ecological interdependence and myriads of underlying interactions. In this review, we describe how species from different kingdoms interact in biofilms and discuss the functional consequences of such interactions. We highlight metabolic advances of collaboration among species from different kingdoms, and advocate that these interactions are of great importance and need to be addressed in future research. Since trans-kingdom biofilms impact diverse contexts, ranging from complicated infections to efficient growth of plants, future knowledge within this field will be beneficial for medical microbiology, biotechnology, and our general understanding of microbial life in nature.".
• 01GT1AV9TWEYN6D00D3HZDA410 abstract "Antibiotic residues and antibiotic resistance genes can enter the environment via fertilization with calf and pig manure. In a longitudinal study, nine antibiotic resistance genes (tet(B), tet(L), tet(M), tet(O), tet(Q), tet(W), erm(B), erm(F) and sul2) and 56 antibiotic residues were investigated in 288 soil samples and 8 corresponding slurry samples from 6 pig farms and 2 veal farms using qPCR and LC-MS/MS, respectively. A significant increase in gene copy number of tet(M), erm(B), erm(F) and sul2 was observed in all the soil layers between sampling times prior to (T1) and 2-3 weeks after fertilization (T3). Tet(B), tet(Q) and tet(L) were least abundant in the soil among the genes tested. From 7 classes of antibiotics, 20 residues were detected in soil and slurry using an optimized and validated extraction method. Flumequine was detected in all soil samples in concentrations below 100 mu g/kg despite being detected in only half of the corresponding slurry samples. Doxycycline, oxytetracycline, lincomycin and sulfadiazine were also frequently detected in concentrations ranging from 0.1 mu g/kg to 500 mu g/kg and from 2 mu g/kg and 9480 mu g/kg in soil and slurry, respectively. Furthermore a positive association between the presence of antibiotic residues (total antibiotic load) and antibiotic resistance genes in soil was found. One possible explanation for this is a simultaneous introduction of antibiotic residues and resistance genes upon application of animal slurry.".
• 01GT1BB6TMVP28EEN3B37NA4HJ abstract "Since climate change impacts are already occurring, urgent adaptive actions are necessary to avoid the worst damages. Regional authorities play an important role in adaptation, but they have few binding guidelines to carry out strategies and plans. Sectoral impacts and adaptive measures strongly differ between regions; therefore, specific results for each territory are needed. Impacts are often not exhaustively reported by literature, dataset and models, thus making it impossible to objectively identify specific adaptive measures. Usual expert elicitation helps to fill this gap but shows some issues. For the Piedmont Strategy, an innovative approach has been proposed, involving experts of private and public bodies (regional authorities, academia, research institutes, parks, associations, NGOs, etc.). They collaborated in two work group, first to identify current and future impacts on biodiversity and ecosystems, and secondly to elaborate and prioritize measures. Involving 143 experts of 46 affiliations, it was possible to quickly edit a cross-validated list of impacts (110) and measures (92) with limited costs. Lastly, a public return of results took place. This approach proved to be effective, efficient and influenced the policymakers, overcoming the tendency to enact long-term actions to face climate change. It could be used internationally by subnational authorities also in other sectors.".
• 01GT1BTVYKWBXBKE4JPSR11KZS abstract "Lifelong movement and physical activity (PA) patterns develop during early childhood. Therefore, educators (teachers and practitioners) in early childhood education and care (ECEC) should provide opportunities to support children’s play, PA, and movement development. The World Health Organization (2019) offers new recommendations for PA, for children under five years. The guidelines do not specify the ways ECEC staff can support PA through play. Therefore, this paper investigates, how physical play (PP) is enacted globally. An international policy and practice analysis of twelve countries, (Australia [Victoria], Belgium [Flanders], Canada [Alberta], China, Finland, Ireland, Italy, Portugal, Spain, Sweden, UK [England] and USA) was completed by analyzing the ECEC curricula and their implementation in different cultural contexts. A content analysis was undertaken by AIESEP Early Years SIG experts revealing that PP was not clearly defined. When defined, it was described as PA, and important for children’s holistic development. The majority of curricula did not state the length/time for PP. Three main strategies for implementing PP were found: a) pedagogical framework; b) active learning methods; and c) motor development. This international analysis highlights the global need for better ECEC staff support in acknowledging and implementing PP to aid children’s overall development, PA and wellbeing.".
• 01GT1EJJKM9VYM28XQ6T4QEW4R abstract "In an international cohort of 279 patients with hypoxemic influenza pneumonia, we identified 13 patients (4.6%) with autoantibodies neutralizing IFN-alpha and/or -omega, which were previously reported to underlie 15% cases of life-threatening COVID-19 pneumonia and one third of severe adverse reactions to live-attenuated yellow fever vaccine. Autoantibodies neutralizing type I interferons (IFNs) can underlie critical COVID-19 pneumonia and yellow fever vaccine disease. We report here on 13 patients harboring autoantibodies neutralizing IFN-alpha 2 alone (five patients) or with IFN-omega (eight patients) from a cohort of 279 patients (4.7%) aged 6-73 yr with critical influenza pneumonia. Nine and four patients had antibodies neutralizing high and low concentrations, respectively, of IFN-alpha 2, and six and two patients had antibodies neutralizing high and low concentrations, respectively, of IFN-omega. The patients' autoantibodies increased influenza A virus replication in both A549 cells and reconstituted human airway epithelia. The prevalence of these antibodies was significantly higher than that in the general population for patients <70 yr of age (5.7 vs. 1.1%, P = 2.2 x 10(-5)), but not >70 yr of age (3.1 vs. 4.4%, P = 0.68). The risk of critical influenza was highest in patients with antibodies neutralizing high concentrations of both IFN-alpha 2 and IFN-omega (OR = 11.7, P = 1.3 x 10(-5)), especially those <70 yr old (OR = 139.9, P = 3.1 x 10(-10)). We also identified 10 patients in additional influenza patient cohorts. Autoantibodies neutralizing type I IFNs account for similar to 5% of cases of life-threatening influenza pneumonia in patients <70 yr old.".
• 01GT1FM1D1D8NAG3AYM46HK4AS abstract "Reason for Performing StudySo far, only transcranial motor evoked potentials (MEP) of the extensor carpi radialis and tibialis cranialis have been documented for diagnostic evaluation in horses. These allow for differentiating whether lesions are located in either the thoraco-lumbar region or in the cervical myelum and/or brain. Transcranial trapezius MEPs further enable to distinguish between spinal and supraspinal located lesions. No normative data are available. It is unclear whether transcranial electrical stimulation (TES) and transcranial magnetic stimulation (TMS) are interchangeable modalities. ObjectivesTo provide normative data for trapezius MEP parameters in horses for TES and TMS and to discern direct and indirect conduction routes by neurophysiological models that use anatomical geometric characteristics to relate latency times with peripheral (PCV) and central conduction velocities (CCV). MethodsTranscranial electrical stimulation-induced trapezius MEPs were obtained from twelve horses. TES and TMS-MEPs (subgroup 5 horses) were compared intra-individually. Trapezius MEPs were measured bilaterally twice at 5 intensity steps. Motoneurons were localized using nerve conduction models of the cervical and spinal accessory nerves (SAN). Predicted CCVs were verified by multifidus MEP data from two horses referred for neurophysiological assessment. ResultsMean MEP latencies revealed for TES: 13.5 (11.1-16.0)ms and TMS: 19.7 (12-29.5)ms, comprising similar to 100% direct routes and for TMS mixed direct/indirect routes of L:23/50; R:14/50. Left/right latency decreases over 10 > 50 V for TES were: -1.4/-1.8 ms and over 10 > 50% for TMS: -1.7/-3.5 ms. Direct route TMS-TES latency differences were 1.88-4.30 ms. 95% MEP amplitudes ranges for TES were: L:0.26-22 mV; R:0.5-15 mV and TMS: L:0.9 - 9.1 mV; R:1.1-7.9 mV. ConclusionThis is the first study to report normative data characterizing TES and TMS induced- trapezius MEPs in horses. The complex trapezius innervation leaves TES as the only reliable stimulation modality. Differences in latency times along the SAN route permit for estimation of the location of active motoneurons, which is of importance for clinical diagnostic purpose. SAN route lengths and latency times are governed by anatomical locations of motoneurons across C2-C5 segments. TES intensity-dependent reductions of trapezius MEP latencies are similar to limb muscles while MEP amplitudes between sides and between TES and TMS are not different. CCVs may reach 180 m/s.".
• 01GT1FY05AC3RT15EEDTT88AEY abstract "Fuzzy rough sets are the fruit of an intense and longlasting collaboration effort between fuzzy set theory and rough set theory. Seminal research on the hybridization originated in the late 1980's, and has inspired generations of researchers from around the globe to address both theoretical and practical challenges. In this paper, we gauge the state-of-the-art in this domain and identify opportunities for further development. In particular, we highlight the potential of fuzzy quantifiers in creating new robust fuzzy rough models, we advocate closer integration with granular computing as a stepping stone for designing rule induction algorithms, and we contemplate the role of fuzzy rough sets vis-a-vis explainable artificial intelligence.".
• 01GT1G2XM7A8HVQSPQ10587ZXM abstract "Stealing thunder has emerged as an essential line of crisis communication research for both crisis managers and crisis researchers. Claeys provides a comprehensive overview of prior research on the stealing thunder effect and its theoretical explanations. Claeys reports that the findings consistently show that self-disclosing a crisis results in less reputational damage than responding to third-party revelations. Claeys notes that while stealing thunder is effective and seems straightforward, simply disclosing anything regarding an ongoing crisis will not do the trick. Claeys discusses the right and wrong ways to steal thunder. This chapter is a valuable resource for future crisis researchers exploring stealing thunder.".
• 01GT1GWSF0T1NJCWYK2ZVENB37 abstract "Fannes and Claeys explore the important role language abstraction plays in the effectiveness of crisis information. The chapter is an important step that helps refine our understanding of how we “operationalize” crisis response strategies – construct the actual crisis response. The chapter uses a product-harm crisis and explores the nature of the product recall through an experiment. Fannes and Claeys compare concrete and abstract language use in the recall message. A product recall containing concrete language results in less reputational damage than a product recall containing abstract language. They argue the difference between concrete and abstract language use is due to an increase of message comprehensibility and source trustworthiness. Concrete language in a product recall results in more reputation repair than abstract language, irrespective of the type of crisis information. The study provides valuable advice for crisis managers seeking to maximize the reputational protection value of the crisis response.".
• 01GT1H8DZ4R40ABD1CCZ15CAWP abstract "Schoofs et al. address the critical topic of empathy in crisis communication. The chapter centers on a qualitative study that adds to their growing research program on empathy. Schoofs et al. begin with the premise that in order to repair a reputation, established crisis communication strategies must be put into practice in the right way. This position reflects a growing concern in crisis communication literature for how crisis responses are operationalized. See Chapter 24 by Fannes and Claeys and Chapter 29 by Tachkova for related discussions of how crisis response strategies are crafted. Schoofs et al. posit that the degree to which crisis responses stimulate reputation repair is associated with perceptions of sincerity. The public needs to feel that the speaker genuinely empathizes with those affected by the crisis in order to be able to forgive. The study found empathy to be critical in facilitating positive reputational outcomes from crisis responses. At the same time, the organization should show competence in handling the crisis. Empathy will not compensate for an incompetent crisis response. Through focus group discussions in which members of the public discuss audiovisual crisis responses, this study explored which aspects of crisis communication that received much prior attention actually matter in shaping public perceptions and in what ways. The findings align with evidence-based guidelines in the sense that apologies, emotions, and nonverbal behaviors are important in shaping public perceptions. However, simply applying recommended strategies may not be enough to repair reputations. Crisis communication strategies must be put to practice in such a way that shows empathy for victims, while the speaker appears both competent and sincere.".
• 01GT1J5WK77C40G7Z3HMTB5WGY abstract "Methanol as a critical piece of the puzzle for realizing net-zero shipping is a vision shared by the FASTWATER consortium, aiming to set a “FAST Track to Clean and Carbon-Neutral WATERborne Transport”. This is a project funded by the European Union’s Horizon 2020 framework program, which aims at demonstrating the entire value chain for using methanol as a marine fuel. This is being done by the development of retrofitted marine engines that operate on methanol and the demonstration of methanol-powered vessels that fulfil all the certification requirements. This paper focuses on the first successful demo vessel of FASTWATER: the pilot boat that is already in service in Oxelösund, Sweden, which is equipped with a compression-ignition (CI) methanol engine. The vessel’s drivability and performance are proving very similar to conventional diesel engines, and its engine is certified for the International Maritime Organization (IMO) Tier III NOx standard without an after-treatment system.".
• 01GT1JGERE0Z6M7XPTDS5AECRX abstract "Simple Summary Toxoplasmosis is a zoonosis primarily transmitted via the consumption of infected/contaminated meat/vegetables/fruits, resulting in several years of disability-adjusted life. In Brazil, a high prevalence of toxoplasmosis has been recorded, and it affects hundreds of people. Several studies have been performed in Brazil to determine the occurrence of toxoplasmosis in animals at different levels of the food chain. However, to fully understand the situation in the country, there is a need to compile the studies performed in abattoirs establishing Toxoplasma gondii prevalence, its risk factors, as well as the infectivity of seropositive animals. Thus, we performed a systematic review gathering data on the prevalence and risk factors for toxoplasmosis in animals slaughtered in Brazil and performed a meta-analysis of its prevalence for different species and regions. Based on the results, we also discussed the infectivity of seropositive animals, risk factors, and the preventive strategies to avoid this zoonosis. Toxoplasmosis is mainly transmitted to human beings through the consumption of contaminated food, and several outbreaks caused by infected meat consumption have been reported in Brazil. We performed a systematic literature review on the prevalence and risk factors for toxoplasmosis in slaughtered animals and performed a meta-analysis of its prevalence for different species and regions. Furthermore, we also discussed the infectivity of seropositive animals, risk factors, and preventive strategies. In the meta-analysis, the overall prevalence estimates for poultry, ostrich, goats, swine, equids, sheep, and bovines were 42.4, 40.4, 23.0, 19.9, 19.1, 17.3, and 16.2%, respectively. Regarding the Brazilian regions, the highest prevalence values were detected for bovines and equids in the South (32.3 and 34.4%, respectively) and swine, goats, sheep, and poultry in the Northeast (29.3, 23.0, 22.9, and 69.8%%, respectively). High proportions of Toxoplasma gondii viability in bioassay conducted on seropositive animals were seen for sheep (34/40 = 85%) and swine (12/15 = 80%). Toxoplasma gondii infections are widespread on Brazilian farms, and the summarized data allow the establishment of high-priority areas and/or species for the adoption of preventive strategies to control this parasite at different levels of the food chain.".
• 01GT1JGES36WMWZ0FQ3KE896VA abstract "BACKGROUND Taenia solium typically affects resource-poor communities where pigs are allowed to roam freely, and sanitation and hygiene levels are suboptimal. Sustainable, long-term strategies are urgently needed to control the disease. Geographically targeted interventions, i.e. screening or treatment of taeniosis among people living near infected pigs (defined as ring screening and ring treatment, respectively), have been shown to be effective control options in Peru. However, these results might not be directly generalizable to sub-Saharan African settings. Pig movements play a vital role in the transmission and, consequently, the success of ring interventions against T. solium. The aim of the present study was to explore roaming patterns of pigs in T. solium endemic communities in Zambia as a first step toward evaluating whether ring interventions should be considered as a treatment option in Zambia. METHODS In total, 48 free-roaming pigs in two rural neighborhoods in the Eastern Province of Zambia were tracked using a Global Positioning System (GPS) receiver. Tracking took place in April (end of the rainy season) 2019 and October (end of the dry season) 2019. The number of revisitations and the time spent within rings of different radii (50, 100 and 250 m) around the coordinates of each pig owner’s household were calculated for each pig. RESULTS The total tracking time for 43 pigs in the final analysis set ranged between 43 and 94 h. Pigs spent a median of 31% and 13% of the tracked time outside the 50- and 100-m radius, respectively, although large variations were observed between pigs. Overall, 25 pigs (58%) went outside the 250-m ring at least once, and individual excursions lasting up to 16 h were observed. In the dry season, 17 out of 23 pigs went outside the 250-m radius compared to only eight out of 20 pigs in the rainy season (P = 0.014). CONCLUSIONS In our study sites in Zambia, the majority of pigs spent most of their time within 50 or 100 m of their owner’s home, and these results are comparable with those on Peruvian pigs. Both radii could therefore be considered reasonable options in future ring interventions.".
• 01GT1JGES7MPZ96EQRY1DS100S abstract "The family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens, encompasses zoonotic nematodes infecting many marine fish. Both are responsible for gastrointestinal disease in humans after ingestion of a live larva by consumption of undercooked fish, and, in the case of A. simplex, an allergic reaction may occur after consuming or even handling infected fish. Due to its phylogenetic relatedness with A. simplex, few studies investigated the allergenic potential of P. decipiens, yet none of them focused on its excretory/secretory (E/S) proteins that easily get missed when working solely on extracts from crushed nematodes. Moreover, these E/S allergens remain behind even when the larva has been removed during fish quality processing. Therefore, the aim was to investigate if Anisakis-like allergens could also be detected in both crushed and E/S P. decipiens protein extract using targeted mass spectrometry analysis and immunological methods. The results confirmed that at least five A. simplex allergens have homologous proteins in P. decipiens; a result that emphasizes the importance of also including E/S protein extracts in proteomic studies. Not only A. simplex, but also P. decipiens should therefore be considered a potential source of allergens that could lead to hypersensitivity reactions in humans.".
• 01GT1KA4RAHC5GD9V1ERC2BJHK abstract "This chapter approaches political discourse as a type of institutional communication. Accordingly, it foregrounds some specific dimensions of political language use: its task-relatedness, the extent to which individuals represent formal organizations, the specialized nature of the communication, and the notions of power and inequality. In this context, it argues that politics does not stand on its own but finds itself in a constant and exciting interaction with other institutional domains and players like the news media, research organizations, NGOs, educational bodies, art institutes and businesses. Drawing on selected case studies on the discursive practices at the intersection between politics, science and news, the chapter demonstrates that it is in studying this very interaction between wide-ranging institutional actors that some of the key discursive features of political discourse can be explored. In particular, it shows how the institutional communication perspective on political discourse can bring added value to the study of communicative genres, discourse processes of identity construction, as well as emerging issues of cross-institutional discursive mediation and participation.".
• 01GT1KJ07QWZH6JA4MQE37X59B abstract "By reappraising the biological theory of vitalism, Canguilhem attempted to give pride of place to the idea that acquiring knowledge about living beings is an activity of living beings. Canguilhem’s legacy is exactly this: rationality is rooted in life, not the other way around. And yet, in “Le concept et la vie” (from 1966) and “De la science et de la contre-science” (from 1971), Canguilhem seems to tell another story about the complex intertwinement of life and rationality. Not only are we condemned to enter the realm of rationality (i.e., to take part in logical activities such as forming concepts and judgments about the world and about our own condition) because we have needs and desires as living beings, but we also have needs and desires as living beings that depend on the fact we are always already caught up in the dynamics of rationality, i.e., always already logically active. At this point, Canguilhem’s thinking comes closer than ever to a Kantian, transcendental point of view on rationality. Paradoxically, the inscription of human rationality in organic life brings with it the idea of a subject, divided between two inverse but correlated realms in which it cannot but participate: the singular realm of sensibility and the general realm of logic.".
• 01GT1PK6ZQH877E7A91D59X8W5 abstract "Background Female genital schistosomiasis (FGS) occurs when Schistosoma haematobium eggs are deposited in reproductive tissue. Female genital schistosomiasis in the cervical mucosa is associated with increased vascularity. If FGS is associated with the presence of hemoglobin in cervicovaginal lavage (CVL), the use of urinary reagent strips to detect hemoglobin in CVL could supplement FGS diagnosis. Methods Nonmenstruating, nonpregnant, sexually active women aged 18-31 participating in the HPTN 071 (PopART) Population-Cohort were invited in 2 Zambian communities. Genital self-swabs and a urine specimen were collected at a home visit, and CVL and hand-held colposcopy were performed at a midwife led clinic visit. Urinary reagent strips were used to identify hemoglobin in CVL. Eggs and circulating anodic antigen (CAA) were detected from urine. Visual-FGS was defined as the presence of sandy patches, rubbery papules, or abnormal blood vessels. Polymerase chain reaction (PCR)-FGS was defined as Schistosoma deoxyribonucleic acid detected by real-time PCR on CVL or cervical or vaginal swab. Results Of 209 women with home genital swabs and companion CVL specimens, 66% (138 of 209) had detectable CVL hemoglobin, 13.4% (28 of 209) had PCR-defined FGS, and 17.2% (36 of 209) had visual-FGS. Active Schistosoma infection, diagnosed by CAA or urine microscopy, was present in 21.0% (44 of 209) participants. Active Schistosoma infection (P = .4), PCR-FGS (P = 0.7), and visual-FGS (P = 0.3) were not associated with CVL hemoglobin presence. Results did not differ in subgroups with high infection burden (cycle threshold < 35 or 2-3 positive genital PCR). Conclusions Polymerase chain reaction-FGS, visual-FGS, and active Schistosoma infection were not associated with the presence of CVL hemoglobin. Further research is needed to establish accessible community-based FGS diagnostics.".
• 01GT1PK70271YYXS5Q6G85AEJF abstract "The neglected tropical disease trichuriasis is caused by the whipworm Trichuris trichiura, a soil-transmitted helminth that has infected humans for millennia. Today, T. trichiura infects as many as 500 million people, predominantly in communities with poor sanitary infrastructure enabling sustained faecal-oral transmission. Using whole-genome sequencing of geographically distributed worms collected from human and other primate hosts, together with ancient samples preserved in archaeologically-defined latrines and deposits dated up to one thousand years old, we present the first population genomics study of T. trichiura. We describe the continent-scale genetic structure between whipworms infecting humans and baboons relative to those infecting other primates. Admixture and population demographic analyses support a stepwise distribution of genetic variation that is highest in Uganda, consistent with an African origin and subsequent translocation with human migration. Finally, genome-wide analyses between human samples and between human and non-human primate samples reveal local regions of genetic differentiation between geographically distinct populations. These data provide insight into zoonotic reservoirs of human-infective T. trichiura and will support future efforts toward the implementation of genomic epidemiology of this globally important helminth.".
• 01GT1PK709RQFZ2VQ2Y9VTQ99H abstract "Author summaryPeriodic follow-up surveys for monitoring and evaluation (M&E) are an important aspect of large-scale neglected tropical disease (NTD) programs. They are critical to measure progress and determine whether continuing or scaling down interventions is justified. In absence of better alternatives, imperfect diagnostic tests are often deployed in these surveys. Yet, little is known how this affects the population-based program decision-making. We expanded an existing survey design framework that allows for imperfect diagnostic tests and applied it to M&E of soil-transmitted helminths control programs as a case study. In addition, we assessed the trade-off between cost per test, sample throughput and diagnostic performance of tests. We demonstrated that the current M&E guidelines may lead to incorrect program decisions, even when perfect diagnostic methods are deployed. To reduce the risk of incorrect decisions, including more disease-clusters and deploying highly specific diagnostic methods were revealed to be the most cost-saving strategies. On the other hand, the higher cost and lower throughput of improved diagnostic tests can be compensated by lower required sample sizes. In conclusion, our framework can contribute to developing more evidence-based guidelines and choices in diagnostic product development for M&E of NTD control programs.".
• 01GT1PK70E3G896F1NETS7Z5DX abstract "BackgroundIt remains largely unknown where and how infections with soil-transmitted helminths (STHs; Ascaris, Trichuris, Necator and Ancylostoma) occur. We therefore aimed to identify possible sources of infection by assessing the environmental contamination in an STH-endemic area. MethodsWe first performed a series of laboratory experiments designed to optimize a soil straining-flotation method to detect and quantify Ascaris and Trichuris eggs in soil, and to validate the diagnostic performance of the optimized method when followed by microscopy and qPCR. In a second phase, we applied this method to assess the level of STH contamination in 399 environmental samples collected from 10 school compounds, 50 households and 9 open markets in Jimma Town (Ethiopia). Subsequently, we explored associations between the environmental contamination and both the corresponding STH epidemiology at the level of the schools and the household characteristics. Finally, we assessed the knowledge, attitude, and practice (KAP) towards STHs in school children. Principal findingsOur soil straining-flotation method has an analytical sensitivity of 50 eggs per 100 grams of soil and egg recovery rate of 36.0% (Ascaris) and 8.0% (Trichuris). The analysis of field samples with both microscopy and qPCR revealed the presence of 8 different helminth species of medical importance, including but not limited to the human STHs. There was a significant association between the environmental contamination and prevalence of any STH infections at the school level only. The KAP indicated a lack of knowledge and awareness of STHs. Conclusions/SignificanceOur optimized straining-flotation method has a moderate diagnostic performance and revealed that life stages of helminths are ubiquitous in the environment, which might be due to the poor sanitary facilities at both the schools and the households, and a poor level of KAP towards STHs. Further research is required to gain more insights into the contribution of these life stages to transmission.".
• 01GT1R6MA1E8CRM49XCBENMPD7 abstract "Previous studies have shown that during visual search, participants are able to implicitly learn across-trial regularities regarding target locations and use these to improve search performance. The present study asks whether such across-trial visual statistical learning also extends to the location of salient distractors. In Experiments 1 and 2, distractor regularities were paired so that a specific distractor location was 100% predictive of another specific distractor location on the next trial. Unlike previous findings that employed target regularities, the current results show no difference in search times between predictable and unpredictable trials. In Experiments 3–5 the distractor location was presented in a structured order (a sequence) for one group of participants, while it was presented randomly for the other group. Again, there was no learning effect of the across-trial regularities regarding the salient distractor locations. Across five experiments, we demonstrated that participants were unable to exploit across-trial spatial regularities regarding the salient distractors. These findings point to important boundary conditions for the modulation of visual attention by statistical regularities and they highlight the need to differentiate between different types of statistical regularities.".
• 01GT1RZQXX4HXV1FQZR4DMDTBK abstract "Objective A late conversation about palliative care needs can lead to suboptimal care in the final months/weeks of life. Insight into factors related to patients' communication about palliative care is needed. This study aims to identify the factors associated with starting/intending to start a conversation about palliative care with the physician. Methods We performed a cross-sectional interviewer-administered survey among people with incurable cancer. Purposive sampling was used, taking into account theoretically relevant heterogeneity. The questionnaire was developed based on the theory of planned behavior. Uni- and multivariable logistic regression analyses were performed. Results Out of 80 participants, ten (13%) started the palliative care conversation and 18 (23%) intended to do so. People holding a positive attitude towards starting/intending to start the conversation (odds ratio [OR] 4.74; 95% CI 2.35-9.54), perceiving more benefits of it (OR 2.60; 95% CI 1.37-4.96) and perceiving a positive attitude towards the behavior in family/friends (OR 2.07; 95% CI 1.26-3.41) and the physician (OR 2.19; 95% CI 1.39-3.45) were more likely to start/intend to start a palliative care conversation; people perceiving more disadvantages (OR 0.53; 95% CI 0.32-0.87) and barriers (OR 0.31; 95% CI 0.15-0.63) were less likely to do so. These factors explained 64% of the variance. Conclusions Our findings show that psychological and perceived socio-environmental factors, particularly patients' attitudes, are associated with starting a conversation about palliative care. Theory-based interventions targeting these strong associations might have a high potential to empower people with cancer to take the initiative in communication about palliative care and to improve timely initiation of palliative care.".
• 01GT1SM0MECAA9YB92VTESTSJB abstract "Purpose: Multiple myeloma (MM) patients with triple-and penta-refractory disease have a poor survival and limited treatment options. Selinexor, in combination with bortezomib and dexamethasone, demonstrated clinical activity in the STOMP study as well as in the BOSTON study in previously treated patients with disease refractory to a proteasome inhibitor (PI). Patients and Methods: Here, we report a real-world case series of 7 heavily pretreated MM patients who had been extensively pretreated with bortezomib and had disease refractory to PIs, including carfilzomib; who were administered a starting dose of 100 mg of selinexor, 20-40 mg dexamethasone and 1.3 mg/m(2) of bortezomib, each once weekly. The majority of these patients (6 patients, 86.0%) had penta-refractory disease, with 5 patients (71.4%) having disease refractory to bortezomib and carfilzomib, and all 7 patients having pomalidomide refractory disease. The median number of prior lines of therapy was 8 (range 4-12). Results: The seven patients in this case series received selinexor for a median of 5 cycles (range 1-10). Four patients (57.1%) had a dose reduction of selinexor. Five patients (71.4%) had a response, of which 2 (29.0%) had a very good partial response (VGPR) and 3 (43.0%) had a partial response (PR). One patient (14.3%) had stable disease (SD) and 1 (14.3%) had progressive disease (PD). There were no new safety signals. Conclusion: The selinexor, bortezomib, and dexamethasone triplet combination demonstrates activity in PI-resistant MM and patients with heavily pretreated MM with refractory disease and after multiple lines of therapy.".
• 01GT1T530920VPED7R1SJKS63T abstract "Long noncoding RNAs (lncRNAs) have increasingly been recognized as being integral to cellular processes, including the antiviral immune response. Porcine reproductive and respiratory syndrome virus (PRRSV) is costly to the global swine industry. To identify PRRSV-related lncRNAs, we performed RNA deep sequencing and compared the profiles of lncRNAs in PRRSV-infected and uninfected Marc-145 cells. We identified a novel lncRNA called MAHAT (maintaining cell morphology-associated and highly conserved antiviral transcript; LTCON_00080558) that inhibits PRRSV replication. MAHAT binds and negatively regulates ZNF34 expression by recruiting and binding DDX6, an RNA helicase forming a complex with ZNF34. Inhibition of ZNF34 expression results in increased type I interferon expression and decreased PRRSV replication. This finding reveals a novel mechanism by which PRRSV evades the host antiviral innate immune response by downregulating the MAHAT-DDX6-ZNF34 pathway. MAHAT could be a host factor target for antiviral therapies against PRRSV infection. IMPORTANCE Long noncoding RNAs (lncRNAs) play important roles in viral infection by regulating the transcription and expression of host genes, and interferon signaling pathways. Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic losses in the swine industry worldwide, but the mechanisms of its pathogenesis and immunology are not fully understood. Here, a new lncRNA, designated MAHAT, was identified as a regulator of host innate immune responses. MAHAT negatively regulates the expression of its target gene, ZNF34, by recruiting and binding DDX6, an RNA helicase, forming a complex with ZNF34. Inhibition of ZNF34 expression increases type I interferon expression and decreases PRRSV replication. This finding suggests that MAHAT has potential as a new target for developing antiviral drugs against PRRSV infection.".
• 01GT1T530GP8M1SNJB79TAAT5C abstract "Viruses may transform infected cells into benign or malignant tumors, promoting cell growth and survival via various intracellular pathways. Some oncogenic DNA viruses, such as human papillomaviruses, can lead to squamous intraepithelial lesions and cervical cancer. Furthermore, the early HPV virus's oncoproteins have been attributed to cancer initiation and development and tumor-enhancing action. In addition to viral oncoproteins, antigen-presenting cells (APC) and the number of clusters of differentiation (CD) markers expressed on their surface play an essential role in disease progression or tumorigenesis inhibition. This article discussed the function of CD markers in the interaction between APCs and cancer cells, immune cells' function in the infection process, and finally infected cells' malignancy. We investigated targeting these markers as a novel insight to create a new therapeutic or diagnosis strategy to prevent cervical cancer progression.".
• 01GT1T530MNBK21DQTQBCH7A2M abstract "Nasal mucosal explant (NEs) cultured at an air-liquid interface mimics in vivo conditions more accurately than monolayer cultures of respiratory cell lines or primary cells cultured in flat-bottom microtiter wells. NEs might be relevant for studies of host-pathogen interactions and antiviral immune responses after infection with respiratory viruses, including influenza and corona viruses. Pigs are natural hosts for swine influenza A virus (IAV) but are also susceptible to IAV from humans, emphasizing the relevance of porcine NEs in the study of IAV infection. Therefore, we performed fundamental characterization and study of innate antiviral responses in porcine NEs using microfluidic high-throughput quantitative real-time PCR (qPCR) to generate expression profiles of host genes involved in inflammation, apoptosis, and antiviral immune responses in mock inoculated and IAV infected porcine NEs. Handling and culturing of the explants ex vivo had a significant impact on gene expression compared to freshly harvested tissue. Upregulation (2-43 fold) of genes involved in inflammation, including IL1A and IL6, and apoptosis, including FAS and CASP3, and downregulation of genes involved in viral recognition (MDA5 (IFIH1)), interferon response (IFNA), and response to virus (OAS1, IFIT1, MX1) was observed. However, by comparing time-matched mock and virus infected NEs, transcription of viral pattern recognition receptors (RIG-I (DDX58), MDA5 (IFIH1), TLR3) and type I and III interferons (IFNB1, IL28B (IFNL3)) were upregulated 2-16 fold in IAV-infected NEs. Furthermore, several interferon-stimulated genes including MX1, o, OAS, OASL, CXCL10, and ISG15 was observed to increase 2-26 fold in response to IAV inoculation. NE expression levels of key genes involved in antiviral responses including IL28B (IFNL3), CXCL10, and OASL was highly comparable to expression levels found in respiratory tissues including nasal mucosa and lung after infection of pigs with the same influenza virus isolate.".
• 01GT1T530STJ69CWDMDJKGZ2EF abstract "Pseudorabies virus (PRV) is the causative agent for Aujeszky's disease, a disease that mainly affects pigs and incidentally other domestic and wild animals. While PRV is almost always fatal, causing neurological disease independently of the age in non-porcine species, the development of neurological manifestation in its host species, the pig, highly depends on the age. In this study, an attempt was made to investigate the effect of nerve development on the outcome of virus infection and the effect of virus infection on the structure of nerves in piglets of various ages. For that reason, 42 pigs at the age of one (n = 14), three (n = 14) and five (n = 14) weeks were inoculated with 10(7) TCID(5)0 of PRV Kaplan strain and euthanized at one- or four-days post inoculation (DPI). The tissues of the trigeminal nervous pathway were collected and examined for virus replication (titration) in cell cultures for nerve morphology by light and transmission electron microscopy, and for viral antigen visualization by immunohistochemistry. The results showed that as the age of the pig increases, virus titers and clinical manifestations reduced, while, at the same time, myelin and axon development ceased. Following infection, the nerve structure was disrupted at all ages examined, being more prominent in one-week-old pigs compared to five-week-old pigs. In conclusion, the age-dependent PRV neuroinvasion in pigs seems to correlate with the morphological changes of neurons.".
• 01GT1T530XZ6K37AFY0WQY1B8D abstract "Objective: The coronavirus disease 2019 (COVID-19) pandemic has resulted in shortages of personal protective equipment (PPE), underscoring the urgent need for simple, efficient, and inexpensive methods to decontaminate masks and respirators exposed to severe acute respiratory coronavirus virus 2 (SARS-CoV-2). We hypothesized that methylene blue (MB) photochemical treatment, which has various clinical applications, could decontaminate PPE contaminated with coronavirus. Design: The 2 arms of the study included (1) PPE inoculation with coronaviruses followed by MB with light (MBL) decontamination treatment and (2) PPE treatment with MBL for 5 cycles of decontamination to determine maintenance of PPE performance. Methods: MBL treatment was used to inactivate coronaviruses on 3 N95 filtering facepiece respirator (FFR) and 2 medical mask models. We inoculated FFR and medical mask materials with 3 coronaviruses, including SARS-CoV-2, and we treated them with 10 mu M MB and exposed them to 50,000 lux of white light or 12,500 lux of red light for 30 minutes. In parallel, integrity was assessed after 5 cycles of decontamination using multiple US and international test methods, and the process was compared with the FDA-authorized vaporized hydrogen peroxide plus ozone (VHP+O-3) decontamination method. Results: Overall, MBL robustly and consistently inactivated all 3 coronaviruses with 99.8% to >99.9% virus inactivation across all FFRs and medical masks tested. FFR and medical mask integrity was maintained after 5 cycles of MBL treatment, whereas 1 FFR model failed after 5 cycles of VHP+O-3. Conclusions: MBL treatment decontaminated respirators and masks by inactivating 3 tested coronaviruses without compromising integrity through 5 cycles of decontamination. MBL decontamination is effective, is low cost, and does not require specialized equipment, making it applicable in low- to high-resource settings.".
• 01GT1TK6JJDWQCGY00B73S9RRC abstract "This paper focuses on the discursive transformations of the semiotic-linguistic landscapes of Pristina, the capital of Kosovo as brought about by successive sociopolitical transformations and against the reversed power relations of the Albanians and Serbs. The study departs from the underlying assumption that the ethnic Albanian image the cityscape emits today does not coincide with the vision of civic inclusion and multi-ethnicity painted in the Constitution (2008) after the Declaration of Independence (2008). By means of a diachronic examination of successive alterations made to iconic landmark establishments at different political phases in time, it is posited that an appreciation of the contemporary discursive landscape requires an understanding of its dialogic relationship with the past. It is contended that semiotic changes reacted to the past by demarcating barriers that limit access to the previously dominant ethnic other. With reference to the ethnically segregated context of the 1990s in Pristina, attention is brought to the transgressive and invisible dimensions of the landscape for the construction of the city's identity today.".
• 01GT1VEPVJFEKV80P4BK9VED6F abstract "This reflection paper complements the WAAVP (World Association for the Advancement of Veterinary Parasitology) general anthelmintic efficacy guideline, which outlines the general principles of anthelmintic efficacy evaluation across all animal host species. It provides background to the recommendations made in the WAAVP general anthelmintic efficacy guideline, with insights into the discussions leading to specific recommendations in the general guideline or the absence thereof. Furthermore, this paper discusses recent technological advancements with potential value to the evaluation of anthelmintic efficacy that may be considered for future versions of the general or species-specific guidelines if supported by sufficient levels of evidence. Finally, it also identifies potential research questions, such as the statistical approach for comparing worm counts between groups of animals.".
• 01GT1VEPVPJ0EDAPW71DGEEWJH abstract "The general WAAVP (World Association for the Advancement of Veterinary Parasitology) guideline on anthelmintic efficacy were prepared to assist researchers with the planning, conduct and interpretation of studies to assess the efficacy of anthelmintic drugs in food-producing and companion animals. General principles are outlined herein to assist in the preparation and execution of dosage determination, dosage confirmation and field studies, which are applicable to all animal host species. These general guidelines are complemented by revised species-specific guidelines, which provide more specific, updated and detailed guidance for each animal host species.".
• 01GT1VEPVT5TD6RHWGW6YB70N4 abstract "Increasing anthelmintic resistance (AR) in livestock has stimulated growing efforts to monitor anthelmintic effectiveness (AE) on livestock farms. On-farm assessment of AE relies on measuring the reduction in faecal egg count (FEC) following treatment; and if conducted rigorously, qualifies as a formal FEC reduction test (FECRT) for AR. Substantial research effort has been devoted to designing robust protocols for the FECRT and its statistical interpretation; however, a wide range of factors other than AR can affect FEC reduction on farms. These are not always possible to control, and can affect the outcome and repeatability of AE measurements and confound the on-farm classification of AR using FECRT. This review considers confounders of FEC reduction, focusing on gastrointestinal nematodes of ruminants, including host and parasite physiology and demography; pharmacokinetic variation between drugs, parasites and hosts; and technical performance. Drug formulation and delivery, host condition and diet, and seasonal variation in parasite species composition, can all affect AE and hence observed FEC reduction. Causes of variation in FEC reduction should be attenuated, but this is not always possible. Regular monitoring of AE can indicate a need to improve anthelmintic administration practices, and detect AR early in its progression. Careful interpretation of FEC reduction, however, taking into account possible confounders, is essential before attributing reduced FEC reduction to AR. Understanding of confounders of FEC reduction will complement advances in FECRT design and interpretation to provide measures of anthelmintic efficacy that are both rigorous and accessible.".
• 01GT1VGPCRMZRYFY8DPGTT26NM abstract "This paper analyses, on the basis of the available archival documents, the early career of Edward A. Shils (1910-1995), especially in the period around 1950, as well as the changing expectations in the field of sociology in this period more generally. It starts with an overview of Shils' translations of German texts, especially of Karl Mannheim and Max Weber, and the way in which he benefited from the growing reputational standing of these authors. The focus next shifts to Shils' transatlantic presence, at both the University of Chicago and the London School of Economics, and the choices he made in this regard. Afterwards we look at his work with Talcott Parsons at Harvard University and the successful last-minute claim to the co-editorship of Toward a General Theory of Action. In the concluding section, it is emphasized that Shils was captivated by the perception of his own life and that of others in terms of a career trajectory - with its specific successes and failures.".
• 01GT1WF6KJNZ6N9BFGN6PKPNXC abstract "In recent decades, cultural heritage research—and in particular art investigation—has been undergoing a digital revolution. This is due both to improvements in the digitization and the acquisition of artifact’s images generated using traditional 2-D imaging methods as well as the growing adoption of a range of more recently introduced spectroscopic imaging techniques. A number of these imaging modalities use wavelengths of electromagnetic radiation that can penetrate surface layers thus yielding information from hidden features noninvasively. Different techniques are often used in combination to provide evidence of construction, condition, and past treatment. These can also be used to characterize the materials used, how they were combined, and map their distribution, giving insight into an artist’s working method and the means to understand changes that have occurred over time. This wealth of data calls for the development of algorithmic approaches in order to handle and fully explore and interpret it. The questions one seeks to answer are in some cases sufficiently different from those addressed in other fields that no existing off-the-shelf approaches can be applied. In this article, we discuss a few of the algorithmic challenges that arise in art investigation and conservation using modern imaging techniques.".
• 01GT1X25D7T3A4GZRH94AQ8K4Y abstract "Telehealth has become an increasingly viable service delivery option for function-based treatment of problem behavior (Lindgren et al., 2016). However, few applications have occurred with participants outside the United States and little research exists evaluating the role that culture plays in service delivery. In the current study, we compared functional analyses and functional communication training delivered via telehealth by ethnically matched and ethnically distinct trainers to six participants in India. We measured the effectiveness using a multiple baseline design while collecting supplemental measures of sessions to criterion, cancellations, treatment fidelity, and social validity. We directly assessed preference for the ethnically matched and ethnically distinct trainers using a concurrent chains arrangement. Sessions with both trainers were effective in reducing problem behavior and increasing functional verbal requests for participating children, and treatment fidelity was high across training modalities. There were no major differences in sessions-to-criterion or cancellations across trainers. However, all six caregivers demonstrated greater preference for sessions with the ethnically matched trainer.".
• 01GT1XNV9VTXRW06Z47D4MT9Q6 abstract "Introduction Excess visceral fat increases the risk of type 2 diabetes and cardiovascular disease and is influenced by sex hormones. Our aim was to investigate changes in visceral fat and the ratio of visceral fat to total body fat (VAT/TBF) and their associations with changes in lipids and insulin resistance after 1 year of hormone therapy in trans persons. Methods In 179 trans women and 162 trans men, changes in total body and visceral fat estimated with dual-energy X-ray absorptiometry before and after 1 year of hormone therapy were related to lipids and insulin resistance [homeostatic model assessment of insulin resistance (HOMA-IR)] with linear regression analysis. Results In trans women, total body fat increased by 4.0 kg (95% CI 3.4, 4.7), while the amount of visceral fat did not change (-2 grams; 95% CI -15, 11), albeit with a large range from -318 to 281, resulting in a decrease in the VAT/TBF ratio of 17% (95% CI 15, 19). In trans men, total body fat decreased with 2.8 kg (95% CI 2.2, 3.5), while the amount of visceral fat did not change (3 g; 95% CI -10, 16; range -372, 311), increasing the VAT/TBF ratio by 14% (95% CI 10, 17). In both groups, VAT/TBF was not associated with changes in blood lipids or HOMA-IR. Conclusions Hormone therapy in trans women and trans men resulted in changes in VAT/TBF, mainly due to changes in total body fat and were unrelated to changes in cardiometabolic risk factors, which suggests that any unfavorable cardiometabolic effects of hormone therapy are not mediated by changes in visceral fat or VAT/TBF.".
• 01GT1YN1GCDV4ZZVA4F03ZFMJ5 abstract "Continued ecosystem damage could eventually make Tigray part of a wider desert connecting the Sahel to the Afar Triangle, a scenario which may render the area uninhabitable.".

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